| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RFC5 |
| Uniprot No | P40937 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-340aa |
| 氨基酸序列 | METSALKQQE QPAATKIRNL PWVEKYRPQT LNDLISHQDI LSTIQKFINE DRLPHLLLYG PPGTGKTSTI LACAKQLYKD KEFGSMVLEL NASDDRGIDI IRGPILSFAS TRTIFKKGFK LVILDEADAM TQDAQNALRR VIEKFTENTR FCLICNYLSK IIPALQSRCT RFRFGPLTPE LMVPRLEHVV EEEKVDISED GMKALVTLSS GDMRRALNIL QSTNMAFGKV TEETVYTCTG HPLKSDIANI LDWMLNQDFT TAYRNITELK TLKGLALHDI LTEIHLFVHR VDFPSSVRIH LLTKMADIEY RLSVGTNEKI QLSSLIAAFQ VTRDLIVAEA |
| 预测分子量 | 38,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RFC5重组蛋白的虚构参考文献示例，格式为文献名称、作者及摘要概述：
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1. **"Cloning and Functional Characterization of Recombinant RFC5 in DNA Replication"**
*Smith A, et al. (2018)*
本研究报道了RFC5重组蛋白在大肠杆菌中的高效表达与纯化，证实其与PCNA结合并促进DNA聚合酶δ的加载，揭示了其在DNA链延伸中的关键作用。
2. **"Structural Insights into RFC5 Subunit in the Replication Factor C Complex"**
*Zhang L, et al. (2020)*
通过X射线晶体学解析了RFC5重组蛋白的三维结构，阐明了其与RFC1亚基的相互作用界面，为理解RFC复合体在DNA损伤修复中的组装机制提供结构基础。
3. **"RFC5 Deficiency Disrupts Genome Stability via Impaired DNA Mismatch Repair"**
*Lee J, et al. (2021)*
利用CRISPR敲除技术，研究发现RFC5重组蛋白缺失导致DNA错配修复效率下降，加剧细胞基因组不稳定性，提示其在癌症预防中的潜在功能。
4. **"RFC5 Recombinant Protein as a Biomarker in Colorectal Cancer Progression"**
*Johnson R, et al. (2022)*
临床样本分析显示，RFC5重组蛋白在结直肠癌组织中表达显著上调，其水平与肿瘤侵袭性正相关，表明其可能作为预后评估的生物标志物。
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注：以上文献为示例，实际引用需检索PubMed、Web of Science等数据库获取真实研究。
RFC5 (Replication Factor C subunit 5) is a critical component of the Replication Factor C (RFC) complex, a conserved AAA+ ATPase essential for DNA replication and repair in eukaryotic cells. The RFC complex, composed of RFC1-RFC5 subunits, functions as a clamp loader that facilitates the assembly of proliferating cell nuclear antigen (PCNA) onto DNA. PCNA, a sliding clamp, coordinates DNA polymerases and repair enzymes during replication and damage repair. RFC5. the smallest subunit, plays a structural and regulatory role in stabilizing the RFC complex and modulating its ATPase activity. Its interaction with other RFC subunits ensures proper recognition of primer-template junctions and ATP-dependent conformational changes required for PCNA loading.
Recombinant RFC5 protein is produced via genetic engineering, typically expressed in *E. coli* or mammalian cell systems, followed by purification using affinity tags (e.g., His-tag). Its recombinant form retains biochemical activity, enabling in vitro studies to dissect RFC-PCNA interaction mechanisms, clamp-loading kinetics, and replication/repair pathway regulation. Researchers utilize RFC5 recombinant protein to investigate genomic instability disorders, cancer biology (where RFC dysregulation is linked to tumorigenesis), and responses to DNA-damaging agents. It also serves as a tool for screening potential anticancer drugs targeting replication machinery. Structural studies using recombinant RFC5 have advanced understanding of RFC complex assembly and its role in DNA metabolism. Additionally, RFC5 mutations or altered expression are implicated in developmental defects and chemotherapy resistance, making its recombinant version valuable for functional validation in disease models.
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