| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PGM2 |
| Uniprot No | Q96G03 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-612aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMAAPEGSGLGEDARLDQETAQWLRWDK NSLTLEAVKRLIAEGNKEELRKCFGARMEFGTAGLRAAMGPGISRMNDLT IIQTTQGFCRYLEKQFSDLKQKGIVISFDARAHPSSGGSSRRFARLAATT FISQGIPVYLFSDITPTPFVPFTVSHLKLCAGIMITASHNPKQDNGYKVY WDNGAQIISPHDKGISQAIEENLEPWPQAWDDSLIDSSPLLHNPSASINN DYFEDLKKYCFHRSVNRETKVKFVHTSVHGVGHSFVQSAFKAFDLVPPEA VPEQKDPDPEFPTVKYPNPEEGKGVLTLSFALADKTKARIVLANDPDADR LAVAEKQDSGEWRVFSGNELGALLGWWLFTSWKEKNQDRSALKDTYMLSS TVSSKILRAIALKEGFHFEETLTGFKWMGNRAKQLIDQGKTVLFAFEEAI GYMCCPFVLDKDGVSAAVISAELASFLATKNLSLSQQLKAIYVEYGYHIT KASYFICHDQETIKKLFENLRNYDGKNNYPKACGKFEISAIRDLTTGYDD SQPDKKAVLPTSKSSQMITFTFANGGVATMRTSGTEPKIKYYAELCAPPG NSDPEQLKKELNELVSAIEEHFFQPQKYNLQPKAD |
| 预测分子量 | 71 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PGM2重组蛋白的3篇参考文献示例(注：以下内容为模拟虚构，实际文献需根据数据库查询结果调整)：
1. **标题**：*Heterologous Expression and Characterization of Recombinant PGM2 in Saccharomyces cerevisiae*
**作者**：Smith J, et al.
**摘要**：研究报道了在酿酒酵母中异源表达PGM2重组蛋白，通过优化密码子使用提高了表达效率，并证明其磷酸葡萄糖变位酶活性对酵母糖代谢通路的调控作用。
2. **标题**：*Purification and Functional Analysis of Recombinant PGM2 from Escherichia coli*
**作者**：Li X, et al.
**摘要**：利用大肠杆菌表达系统成功纯化PGM2重组蛋白，通过酶动力学实验证实其催化葡萄糖-1-磷酸与葡萄糖-6-磷酸转化的高效性，并分析了温度对酶稳定性的影响。
3. **标题**：*Structural Insights into PGM2 Recombinant Protein by X-ray Crystallography*
**作者**：Wang Y, et al.
**摘要**：通过X射线晶体学解析了PGM2重组蛋白的三维结构，揭示了其底物结合位点及催化机制，为设计针对代谢疾病的抑制剂提供了结构基础。
如需具体文献，建议在PubMed、Web of Science等平台检索关键词“PGM2 recombinant protein”或“PGM2 heterologous expression”。
**Background of PGM2 Recombinant Protein**
Phosphoglucomutase 2 (PGM2) is a critical enzyme in carbohydrate metabolism, catalyzing the interconversion of glucose-1-phosphate (G1P) and glucose-6-phosphate (G6P). This reversible reaction serves as a metabolic bridge between glycogen/sugar nucleotide synthesis and glycolysis, making PGM2 essential for maintaining energy homeostasis and sugar nucleotide pools in eukaryotic cells. In humans, PGM2 dysfunction has been linked to metabolic disorders, while in yeast and bacteria, it influences microbial survival under stress.
The recombinant form of PGM2 is produced using genetic engineering techniques, where the *PGM2* gene is cloned into expression vectors and expressed in host systems like *E. coli* or yeast. This allows large-scale production of the purified enzyme for research and industrial applications. Recombinant PGM2 retains the catalytic efficiency of the native enzyme and is often engineered with tags (e.g., His-tag) to facilitate purification.
In biotechnology, PGM2 recombinant protein is leveraged to optimize metabolic pathways in microbial cell factories. For example, in biofuel production (e.g., ethanol), enhancing PGM2 activity can improve carbon flux toward glycolysis, boosting yield. It also plays a role in synthesizing nucleotide sugars, precursors for glycoconjugates used in pharmaceuticals. Additionally, studies on recombinant PGM2 provide insights into enzyme kinetics, substrate specificity, and allosteric regulation, aiding drug development for metabolic diseases or cancer, where altered sugar metabolism is a hallmark.
Overall, PGM2 recombinant protein serves as a versatile tool in both basic science and applied industries, bridging gaps in understanding cellular metabolism and enabling bioengineering advancements.
×
