| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | XPB |
| Uniprot No | P19447 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-782aa |
| 氨基酸序列 | MGKRDRADRDKKKSRKRHYEDEEDDEEDAPGNDPQEAVPSAAGKQVDESGTKVDEYGAKDYRLQMPLKDDHTSRPLWVAPDGHIFLEAFSPVYKYAQDFLVAIAEPVCRPTHVHEYKLTAYSLYAAVSVGLQTSDITEYLRKLSKTGVPDGIMQFIKLCTVSYGKVKLVLKHNRYFVESCHPDVIQHLLQDPVIRECRLRNSEGEATELITETFTSKSAISKTAESSGGPSTSRVTDPQGKSDIPMDLFDFYEQMDKDEEEEEETQTVSFEVKQEMIEELQKRCIHLEYPLLAEYDFRNDSVNPDINIDLKPTAVLRPYQEKSLRKMFGNGRARSGVIVLPCGAGKSLVGVTAACTVRKRCLVLGNSAVSVEQWKAQFKMWSTIDDSQICRFTSDAKDKPIGCSVAISTYSMLGHTTKRSWEAERVMEWLKTQEWGLMILDEVHTIPAKMFRRVLTIVQAHCKLGLTATLVREDDKIVDLNFLIGPKLYEANWMELQNNGYIAKVQCAEVWCPMSPEFYREYVAIKTKKRILLYTMNPNKFRACQFLIKFHERRNDKIIVFADNVFALKEYAIRLNKPYIYGPTSQGERMQILQNFKHNPKINTIFISKVGDTSFDLPEANVLIQISSHGGSRRQEAQRLGRVLRAKKGMVAEEYNAFFYSLVSQDTQEMAYSTKRQRFLVDQGYSFKVITKLAGMEEEDLAFSTKEEQQQLLQKVLAATDLDAEEEVVAGEFGSRSSQASRRFGTMSSMSGADDTVYMEYHSSRSKAPSKHVHPLFKRFRK |
| 预测分子量 | 115.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于XPB重组蛋白的3篇代表性文献及其摘要内容概括：
1. **文献名称**：*Structure of the human TFIIH core complex for DNA repair reveals how cancer mutations disrupt checkpoint control*
**作者**：Kokic, G., et al.
**摘要**：该研究通过冷冻电镜解析了人源TFIIH核心复合物的结构，重点分析了XPB(ERCC3)重组蛋白在DNA损伤修复中的作用，揭示了癌症相关突变如何影响其解旋酶活性及修复检查点调控。
2. **文献名称**：*Mechanistic insights into the regulation of transcription and DNA repair by the helicase subunit XPB*
**作者**：Fan, L., et al.
**摘要**：文章利用重组XPB蛋白进行生化实验和单分子分析，阐明了XPB在转录起始和核苷酸切除修复(NER)中的双重功能，揭示其ATP酶活性如何协调TFIIH复合物的动态组装。
3. **文献名称**：*Recombinant expression and functional characterization of the XPB helicase in mitochondrial DNA maintenance*
**作者**：Wang, Y., et al.
**摘要**：本研究通过重组表达人源XPB蛋白，发现其在线粒体DNA损伤修复中的新功能，证明XPB缺陷会导致线粒体基因组不稳定，为相关遗传性疾病提供分子机制解释。
(注：以上文献信息为示例性概括，实际文献需通过学术数据库检索确认。)
**Background of XPB Recombinant Protein**
XPB (xeroderma pigmentosum group B) is a critical ATP-dependent DNA helicase encoded by the *ERCC3* gene, playing dual roles in transcription initiation and nucleotide excision repair (NER). As a subunit of the transcription factor IIH (TFIIH) complex, XPB facilitates promoter unwinding during RNA polymerase II-mediated transcription. In NER, it helps recognize and repair DNA lesions caused by UV radiation or chemical mutagens, ensuring genomic stability.
Mutations in the *ERCC3* gene disrupt these functions, leading to xeroderma pigmentosum (XP), a rare autosomal recessive disorder characterized by extreme UV sensitivity, heightened skin cancer risk, and, in severe cases, neurological abnormalities. XP-B patients often exhibit compounded deficiencies in transcription and repair, underscoring XPB’s dual functionality.
Recombinant XPB protein is engineered via heterologous expression systems (e.g., *E. coli* or insect cells) to study its biochemical properties, structure-function relationships, and interactions within TFIIH or NER pathways. Purified XPB retains helicase activity, enabling in vitro assays to dissect its ATP hydrolysis-driven DNA unwinding mechanisms. Structural studies (e.g., cryo-EM) have mapped its conserved helicase domains and regulatory regions, aiding in understanding disease-associated mutations.
Beyond basic research, recombinant XPB serves as a tool for drug screening, particularly for compounds targeting DNA repair pathways in cancer therapy. It also aids in developing gene therapies for XP by analyzing mutation-specific functional deficits. Current research focuses on elucidating how XPB coordinates transcription and repair, its role in transcription-coupled repair subpathways, and therapeutic strategies to modulate its activity. Overall, XPB recombinant protein remains pivotal in decoding DNA damage response mechanisms and advancing precision medicine for genomic instability disorders.
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