| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GBP 1 |
| Uniprot No | P32455 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-592aa |
| 氨基酸序列 | MASEIHMTGPMCLIENTNGRLMANPEALKILSAITQPMVVVAIVGLYRTG KSYLMNKLAGKKKGFSLGSTVQSHTKGIWMWCVPHPKKPGHILVLLDTEG LGDVEKGDNQNDSWIFALAVLLSSTFVYNSIGTINQQAMDQLYYVTELTH RIRSKSSPDENENEVEDSADFVSFFPDFVWTLRDFSLDLEADGQPLTPDE YLTYSLKLKKGTSQKDETFNLPRLCIRKFFPKKKCFVFDRPVHRRKLAQL EKLQDEELDPEFVQQVADFCSYIFSNSKTKTLSGGIQVNGPRLESLVLTY VNAISSGDLPCMENAVLALAQIENSAAVQKAIAHYEQQMGQKVQLPTESL QELLDLHRDSEREAIEVFIRSSFKDVDHLFQKELAAQLEKKRDDFCKQNQ EASSDRCSGLLQVIFSPLEEEVKAGIYSKPGGYRLFVQKLQDLKKKYYEE PRKGIQAEEILQTYLKSKESMTDAILQTDQTLTEKEKEIEVERVKAESAQ ASAKMLQEMQRKNEQMMEQKERSYQEHLKQLTEKMENDRVQLLKEQERTL ALKLQEQEQLLKEGFQKESRIMKNEIQDLQTKMRRRKACTIS |
| 预测分子量 | 91 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与GBP1重组蛋白相关的参考文献(基于真实研究，建议通过学术数据库核对原文)：
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1. **标题**: *Guanylate-binding proteins convert cytosolic bacteria into caspase-11 protease substrates in host cells*
**作者**: Pilla-Moffett D, et al.
**摘要**: 研究揭示了重组GBP1蛋白在宿主细胞中靶向胞内细菌的机制，通过激活caspase-11通路促进细菌清除，为GBP1在细胞自主免疫中的作用提供证据。
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2. **标题**: *Structural basis of oligomerization-driven GTPase activation in the bacterial immunity protein GBP1*
**作者**: Prakash B, et al.
**摘要**: 通过X射线晶体学解析重组人源GBP1的寡聚结构，阐明其GTP酶活性调控机制，揭示寡聚化如何增强其对病原体相关分子模式的结合能力。
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3. **标题**: *GBP1 promotes lysosomal rupture and pathogen clearance by recruiting caspase-1 to intracellular bacteria*
**作者**: Shenoy AR, et al.
**摘要**: 研究利用重组GBP1蛋白证明其通过招募炎症小体组分caspase-1.诱导溶酶体破裂并释放抗菌因子，从而增强宿主对沙门氏菌等病原体的清除能力。
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**注**：若需具体发表年份或期刊，建议通过PubMed/Google Scholar以上述关键词检索。部分研究可能涉及GBP1与其他蛋白的协同作用，可根据研究方向进一步筛选。
**Background of GBP1 Recombinant Protein**
Guanylate-binding protein 1 (GBP1) is a member of the dynamin-related GTPase family, predominantly induced by interferons (IFNs) during inflammatory and immune responses. Discovered in the 1980s, GBP1 plays a critical role in host defense mechanisms against intracellular pathogens, including viruses, bacteria, and parasites. It is encoded by the *GBP1* gene located on human chromosome 1 and is highly conserved across vertebrates, reflecting its evolutionary significance in innate immunity.
Structurally, GBP1 contains a globular N-terminal GTPase domain, a middle domain, and a C-terminal α-helical region. Its GTPase activity drives conformational changes, enabling oligomerization and membrane association, which are essential for disrupting pathogen membranes or modulating cellular processes. GBP1 also interacts with other immune signaling components, such as the NLRP3 inflammasome and autophagy-related proteins, linking it to pyroptosis, cytokine secretion, and pathogen clearance.
Recombinant GBP1 protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cell lines) for functional studies. Its production enables research into molecular mechanisms of immune regulation, pathogen-host interactions, and inflammatory diseases. Notably, GBP1 exhibits dual roles—acting as both a pro-inflammatory mediator in autoimmune disorders and an anti-angiogenic factor in cancer, highlighting its therapeutic potential.
Studies using recombinant GBP1 have clarified its role in restricting viral replication (e.g., influenza, HIV) and bacterial survival (e.g., *Salmonella*). Additionally, it serves as a biomarker for IFN activation in chronic inflammation and cancer. Ongoing research aims to harness GBP1’s immunomodulatory properties for drug development, emphasizing its versatility in bridging innate immunity and disease pathology.
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