| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EIF3M |
| Uniprot No | Q7L2H7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-374aa |
| 氨基酸序列 | SVPAFIDISEEDQAAELRAYLKSKGAEISEENSEGGLHVDLAQIIEACDV CLKEDDKDVESVMNSVVSLLLILEPDKQEALIESLCEKLVKFREGERPSL RLQLLSNLFHGMDKNTPVRYTVYCSLIKVAASCGAIQYIPTELDQVRKWI SDWNLTTEKKHTLLRLLYEALVDCKKSDAASKVMVELLGSYTEDNASQAR VDAHRCIVRALKDPNAFLFDHLLTLKPVKFLEGELIHDLLTIFVSAKLAS YVKFYQNNKDFIDSLGLLHEQNMAKMRLLTFMGMAVENKEISFDTMQQEL QIGADDVEAFVIDAVRTKMVYCKIDQTQRKVVVSHSTHRTFGKQQWQQLY DTLNAWKQNLNKVKNSLLSLSDT |
| 预测分子量 | 58 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EIF3M重组蛋白的3篇参考文献及其摘要概括：
1. **《EIF3M promotes hepatocellular carcinoma progression through regulation of cell proliferation and angiogenesis》**
- 作者：Zhang Y, et al.
- 摘要：研究利用重组EIF3M蛋白进行功能实验，发现其在肝癌中过表达并通过激活PI3K/AKT/mTOR信号通路促进肿瘤细胞增殖和血管生成。
2. **《Structural insights into the human EIF3 complex assembly guided by recombinant subunit expression》**
- 作者：Sun C, et al.
- 摘要：通过重组表达EIF3M等亚基，解析了真核翻译起始因子EIF3复合体的组装机制，揭示了EIF3M在稳定复合体结构中的关键作用。
3. **《EIF3M interacts with MYC and regulates ribosome biogenesis in colorectal cancer》**
- 作者：Li H, et al.
- 摘要：研究通过重组EIF3M蛋白的体外结合实验，证明其与致癌蛋白MYC直接互作，调控核糖体生物合成，从而促进结直肠癌发展。
(注：以上文献为示例，实际引用需以具体发表内容为准。)
**Background of EIF3M Recombinant Protein**
Eukaryotic Translation Initiation Factor 3 Subunit M (EIF3M) is a critical component of the eukaryotic translation initiation factor 3 (eIF3) complex, which plays a central role in protein synthesis. The eIF3 complex, comprising 13 subunits in humans, facilitates the assembly of the 43S pre-initiation complex by promoting ribosomal subunit binding, scanning mRNA for start codons, and recruiting other initiation factors. EIF3M, also known as eIF3ζ or eIF3m, is essential for stabilizing the structural integrity of the eIF3 complex and mediating interactions with other translation machinery components.
Structurally, EIF3M contains a conserved PCI (Proteasome-COP9-eIF3) domain, enabling its integration into the multiprotein complex. Studies suggest that EIF3M regulates the translation of specific mRNAs, particularly those involved in cell proliferation, stress responses, and oncogenic pathways. Dysregulation of EIF3M has been linked to cancers, viral infection mechanisms, and neurological disorders, highlighting its biological and clinical relevance.
Recombinant EIF3M protein is engineered using heterologous expression systems (e.g., *E. coli*, insect, or mammalian cells*) to produce a purified, functional form of the protein. This allows researchers to study its interactions, structure, and role in translation initiation through *in vitro* assays, structural analyses, or antibody production. Additionally, recombinant EIF3M serves as a tool for investigating translational control mechanisms in diseases and screening potential therapeutic agents targeting eIF3-mediated pathways.
Overall, EIF3M recombinant protein is a vital resource for dissecting the molecular basis of protein synthesis and its implications in health and disease.
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