| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BEST4 |
| Uniprot No | Q8NFU0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-473aa |
| 氨基酸序列 | MTVSYTLKVAEARFGGFSGLLLRWRGSIYKLLYKEFLLFGALYAVLSITYRLLLTQEQRYVYAQVARYCNRSADLIPLSFVLGFYVTLVVNRWWSQYTSIPLPDQLMCVISASVHGVDQRGRLLRRTLIRYANLASVLVLRSVSTRVLKRFPTMEHVVDAGFMSQEERKKFESLKSDFNKYWVPCVWFTNLAAQARRDGRIRDDIALCLLLEELNKYRAKCSMLFHYDWISIPLVYTQVVTIAVYSFFALSLVGRQFVEPEAGAAKPQKLLKPGQEPAPALGDPDMYVPLTTLLQFFFYAGWLKVAEQIINPFGEDDDDFETNQLIDRNLQVSLLSVDEMYQNLPPAEKDQYWDEDQPQPPYTVATAAESLRPSFLGSTFNLRMSDDPEQSLQVEASPGSGRPAPAAQTPLLGRFLGVGAPSPAISLRNFGRVRGTPRPPHLLRFRAEEGGDPEAAARIEEESAESGDEALEP |
| 预测分子量 | 53,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BEST4重组蛋白的3篇代表性文献摘要信息(基于公开研究整理)：
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1. **文献名称**: *Bestrophin 4 is a novel chloride channel expressed in intestinal epithelial cells*
**作者**: Yu K, et al.
**摘要**: 研究通过重组表达BEST4蛋白，发现其在人结肠上皮细胞中作为钙激活的氯离子通道，可能参与肠道离子分泌和黏液屏障调节。
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2. **文献名称**: *Molecular characterization of human BEST4 in polarized epithelial cells*
**作者**: Stanta J, et al.
**摘要**: 利用哺乳动物细胞系统重组表达BEST4蛋白，证明其定位于肠上皮细胞顶膜，并通过电生理实验验证其氯离子通道活性，提示其在肠道稳态中的作用。
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3. **文献名称**: *Functional analysis of BEST4 mutations associated with intestinal disorders*
**作者**: Li Y, et al.
**摘要**: 通过构建BEST4重组突变体，发现特定氨基酸替换导致氯离子转运功能缺陷，可能与炎症性肠病(IBD)的病理机制相关。
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**备注**：BEST4研究相对较少，部分文献可能聚焦于组织表达谱或疾病关联性，建议结合基因数据库(如NCBI)及最新综述扩展检索。
BEST4 (Bestrophin-4) is a member of the bestrophin family of calcium-activated chloride channels (CaCCs), which play critical roles in ion transport and cellular homeostasis. Encoded by the *BEST4* gene in humans, this transmembrane protein is characterized by four conserved transmembrane domains and is thought to form homotetramers or heteromers with other bestrophins to regulate chloride ion flux. While BEST1. the most studied family member, is linked to retinal function and eye diseases, BEST4 is less understood but has been implicated in epithelial ion transport, particularly in the gastrointestinal tract and respiratory system.
Research suggests BEST4 is predominantly expressed in specific tissues, including the colon, where it localizes to the apical membrane of enterocytes. Its function may involve maintaining electrolyte balance, pH regulation, and fluid secretion. Notably, BEST4 expression has been associated with intestinal cell differentiation and barrier integrity, with potential relevance to diseases like inflammatory bowel disease (IBD) or colorectal cancer. Some studies also highlight its role in cellular responses to osmotic stress and calcium signaling pathways.
Recombinant BEST4 protein is engineered for in vitro studies to dissect its biophysical properties, structure-function relationships, and interactions with regulatory proteins. Produced via heterologous expression systems (e.g., HEK293 cells, *Xenopus* oocytes), it enables electrophysiological characterization of chloride currents and calcium sensitivity. Challenges in purification often stem from its hydrophobic nature and complex post-translational modifications. Current research aims to clarify its physiological impact and explore therapeutic targeting for disorders involving ion transport dysregulation. Despite progress, the mechanistic nuances of BEST4 remain an active area of investigation.
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