| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Glu |
| Uniprot No | Q9H093 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-628aa |
| 氨基酸序列 | MESLVFARRSGPTPSAAELARPLAEGLIKSPKPLMKKQAVKRHHHKHNLRHRYEFLETLGKGTYGKVKKARESSGRLVAIKSIRKDKIKDEQDLMHIRREIEIMSSLNHPHIIAIHEVFENSSKIVIVMEYASRGDLYDYISERQQLSEREARHFFRQIVSAVHYCHQNRVVHRDLKLENILLDANGNIKIADFGLSNLYHQGKFLQTFCGSPLYASPEIVNGKPYTGPEVDSWSLGVLLYILVHGTMPFDGHDHKILVKQISNGAYREPPKPSDACGLIRWLLMVNPTRRATLEDVASHWWVNWGYATRVGEQEAPHEGGHPGSDSARASMADWLRRSSRPLLENGAKVCSFFKQHAPGGGSTTPGLERQHSLKKSRKENDMAQSLHSDTADDTAHRPGKSNLKLPKGILKKKVSASAEGVQEDPPELSPIPASPGQAAPLLPKKGILKKPRQRESGYYSSPEPSESGELLDAGDVFVSGDPKEQKPPQASGLLLHRKGILKLNGKFSQTALELAAPTTFGSLDELAPPRPLARASRPSGAVSEDSILSSESFDQLDLPERLPEPPLRGCVSVDNLTGLEEPPSEGPGSCLRRWRQDPLGDSCFSLTDCQEVTATYRQALRVCSKLT |
| 预测分子量 | 69,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于谷氨酸(Glu)相关重组蛋白研究的3篇代表性文献示例(文献信息为模拟虚构，仅供参考格式)：
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1. **文献名称**：*Expression and Purification of Recombinant Glutamate Dehydrogenase from Escherichia coli*
**作者**：Smith, J.R., et al.
**摘要**：本研究报道了一种高效表达和纯化重组谷氨酸脱氢酶(GDH)的方法，通过优化大肠杆菌表达系统，获得高活性GDH，并应用于体外谷氨酸代谢通路的功能分析。
2. **文献名称**：*Structural Insights into the Ion Permeation Mechanism of GluN2B-Containing NMDA Receptors*
**作者**：Chen, L., & Gouaux, E.
**摘要**：通过重组表达GluN2B亚基的NMDA受体，结合冷冻电镜技术解析其三维结构，揭示了谷氨酸结合后离子通道开放的分子机制，为神经疾病药物开发提供依据。
3. **文献名称**：*Engineering a Thermostable Glutamate Decarboxylase for Industrial GABA Production*
**作者**：Wang, Y., et al.
**摘要**：利用定向进化技术改造重组谷氨酸脱羧酶(GAD)，提高其热稳定性和催化效率，成功应用于γ-氨基丁酸(GABA)的大规模生物合成。
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**注**：以上文献为示例性内容，实际研究中请通过PubMed或Web of Science等平台检索真实发表的论文。
Glu recombinant proteins, often referring to glutamate-related engineered proteins, are artificially synthesized molecules designed to study or utilize glutamate-mediated biological processes. Glutamate, a pivotal neurotransmitter in the central nervous system, plays critical roles in synaptic transmission, learning, and memory. Recombinant Glu proteins, such as glutamate receptors (e.g., NMDA, AMPA, or kainate receptors), transporters, or metabolic enzymes, are generated using recombinant DNA technology. These proteins are typically expressed in heterologous systems like Escherichia coli, yeast, or mammalian cells to ensure proper folding and post-translational modifications.
The development of Glu recombinant proteins has advanced neuroscience research by enabling structural studies (e.g., cryo-EM analyses of receptor conformations), drug discovery (screening for neuroactive compounds), and mechanistic insights into neurological disorders such as epilepsy, Alzheimer’s disease, and schizophrenia. For instance, engineered Glu receptors help dissect ligand-binding domains or allosteric modulation sites, guiding the design of targeted therapies.
Challenges include maintaining functional integrity during purification, achieving native-like oligomerization, and replicating tissue-specific modifications. Recent innovations, like cell-free expression systems and glycoengineering in mammalian platforms, address these issues, enhancing protein stability and activity.
Beyond neuroscience, Glu recombinant proteins find applications in industrial biocatalysis (e.g., glutamate decarboxylase for GABA production) and biosensors for environmental monitoring. Their versatility underscores their importance in bridging basic research and translational innovations.
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