| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DNS |
| Uniprot No | O00115 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 19-360aa |
| 氨基酸序列 | CY GDSGQPVDWF VVYKLPALRG SGEAAQRGLQ YKYLDESSGG WRDGRALINS PEGAVGRSLQ PLYRSNTSQL AFLLYNDQPP QPSKAQDSSM RGHTKGVLLL DHDGGFWLVH SVPNFPPPAS SAAYSWPHSA CTYGQTLLCV SFPFAQFSKM GKQLTYTYPW VYNYQLEGIF AQEFPDLENV VKGHHVSQEP WNSSITLTSQ AGAVFQSFAK FSKFGDDLYS GWLAAALGTN LQVQFWHKTV GILPSNCSDI WQVLNVNQIA FPGPAGPSFN STEDHSKWCV SPKGPWTCVG DMNRNQGEEQ RGGGTLCAQL PALWKAFQPL VKNYQPCNGM ARKPSRAYKI |
| 预测分子量 | 39,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DNA重组蛋白的3篇参考文献示例(注：文献为模拟示例，建议通过学术数据库获取真实文献)：
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1. **文献名称**: "Mechanism of Homologous Recombination in Escherichia coli: The RecA Protein"
**作者**: Cox, M. M.
**摘要**: 该综述系统阐述了RecA蛋白在大肠杆菌同源重组中的作用机制，包括其促进DNA链交换、修复双链断裂的功能，并讨论了其在基因组稳定性中的重要性。
2. **文献名称**: "Cre Recombinase: A Versatile Tool for Genome Engineering"
**作者**: Sternberg, N., & Hamilton, D.
**摘要**: 本文详细描述了Cre重组酶的特性及其在loxP位点介导的DNA特异性重组中的应用，涵盖其在转基因动物模型构建和基因功能研究中的关键技术突破。
3. **文献名称**: "Recombinant Protein Expression in Escherichia coli: Advances and Challenges"
**作者**: Rosano, G. L., & Ceccarelli, E. A.
**摘要**: 分析了大肠杆菌表达系统中重组蛋白生产的优化策略，包括启动子选择、密码子优化和融合标签设计，同时探讨了提高可溶性和活性的方法。
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**建议**：若需具体领域文献，可通过PubMed、Google Scholar等平台搜索关键词(如“recombinant DNA proteins”“site-specific recombination”)，或结合特定蛋白名称(如RecA、Cre、Cas9)获取最新研究。
**Background of DNS Recombinant Protein**
DNS recombinant protein is a engineered biomolecule derived from the fusion or modification of specific protein domains, often involving *DNA-binding*, *nuclease*, or *structural* elements. The term "DNS" may refer to a functional composite, such as a protein integrating DNA-interaction motifs (e.g., zinc fingers or helix-turn-helix regions) with enzymatic or signaling domains, though its exact definition varies by context. Recombinant DNS proteins are typically designed to study or manipulate biological processes like DNA repair, gene regulation, or pathogen defense.
The development of DNS recombinant proteins leverages advances in genetic engineering, including codon optimization, fusion-tag systems (e.g., His-tags for purification), and heterologous expression in bacterial, yeast, or mammalian hosts. Such proteins are pivotal in structural biology (e.g., crystallography of DNA-protein complexes) and biotechnology applications, such as targeted genome editing or antiviral therapies. For instance, engineered nucleases (e.g., CRISPR-associated proteins) or DNA-binding scaffolds often incorporate DNS-like features to enhance specificity or functionality.
Research on DNS recombinant proteins also addresses challenges like protein stability, solubility, and functional fidelity *in vitro* or *in vivo*. Their utility spans diagnostics (e.g., biosensors), therapeutics (e.g., gene therapy vectors), and synthetic biology. By mimicking natural protein-DNA interactions or introducing novel functions, these molecules exemplify the convergence of molecular biology and bioengineering, driving innovations in precision medicine and biomanufacturing.
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*Note: The term "DNS" is context-dependent; this summary assumes a generalized interpretation common in protein engineering literature.*
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