| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HMGN5 |
| Uniprot No | P82970 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-282aa |
| 氨基酸序列 | MPKRKAAGQG DMRQEPKRRS ARLSAMLVPV TPEVKPKRTS SSRKMKTKSD MMEENIDTSA QAVAETKQEA VVEEDYNENA KNGEAKITEA PASEKEIVEV KEENIEDATE KGGEKKEAVA AEVKNEEEDQ KEDEEDQNEE KGEAGKEDKD EKGEEDGKED KNGNEKGEDA KEKEDGKKGE DGKGNGEDGK EKGEDEKEEE DRKETGDGKE NEDGKEKGDK KEGKDVKVKE DEKEREDGKE DEGGNEEEAG KEKEDLKEEE EGKEEDEIKE DDGKKEEPQS IV |
| 预测分子量 | 31,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HMGN5重组蛋白的3篇代表性文献示例(内容基于学术研究常见方向，仅供参考)：
---
1. **文献名称**：*HMGN5 enhances chromatin accessibility and promotes nuclear reprogramming*
**作者**：Zhang Y. et al.
**摘要**：本研究成功在大肠杆菌中表达并纯化了重组HMGN5蛋白，验证其通过破坏核小体结构增强染色质可及性的能力。实验表明，HMGN5重组蛋白可协同转录因子Oct4/Sox2提升体细胞重编程为多能干细胞的效率，为染色质调控机制提供了新见解。
---
2. **文献名称**：*Recombinant HMGN5 modulates DNA damage response in cancer cells*
**作者**：Singh R. et al.
**摘要**：通过哺乳动物细胞系统表达HMGN5重组蛋白，发现其过表达导致癌细胞DNA损伤修复能力下降，并增加放疗敏感性。研究揭示了HMGN5通过干扰组蛋白H1的分布影响DNA损伤信号通路，提示其作为癌症治疗潜在靶点。
---
3. **文献名称**：*Biophysical characterization of HMGN5-nucleosome interactions*
**作者**：Lee S. et al.
**摘要**：利用重组HMGN5蛋白进行原子力显微镜和荧光共振能量转移实验，证明其通过C端结构域与核小体核心颗粒动态结合，显著降低染色质纤维的压缩程度。该研究阐明了HMGN5调控基因组三维结构的分子机制。
---
**注**：以上文献为模拟示例，实际研究中建议通过PubMed或Web of Science检索最新成果(可尝试关键词：HMGN5 recombinant, HMGN5 chromatin, HMGN5 nucleosome)。真实文献需核对作者、期刊及具体实验细节。
HMGN5. a member of the High Mobility Group Nucleosome-Binding (HMGN) protein family, is a chromatin architectural protein involved in regulating chromatin structure and gene expression. It binds to nucleosomes without sequence specificity, modulating DNA accessibility for transcription factors and epigenetic modifiers. HMGN5. also known as NSBP1 (Nucleosome-Binding Protein 1), is characterized by a conserved nucleosome-binding domain and a C-terminal regulatory region. Its dynamic interaction with chromatin influences transcriptional activation, DNA repair, and epigenetic memory.
Recombinant HMGN5 protein is produced using expression systems like *E. coli* or mammalian cells, enabling controlled studies of its biochemical and functional properties. Researchers utilize it to investigate its role in chromatin decompaction, a process critical for cellular differentiation, development, and stress responses. Studies highlight its involvement in diseases, particularly cancer, where dysregulated HMGN5 expression correlates with tumor progression. For example, overexpression in certain cancers enhances oncogene transcription by promoting chromatin relaxation.
The recombinant form is essential for *in vitro* assays, such as nucleosome-binding studies, chromatin remodeling assays, and protein interaction analyses. Its application extends to exploring cross-talk with histone modifications (e.g., acetylation, methylation) and testing therapeutic inhibitors targeting chromatin dynamics. Recent work also links HMGN5 to stem cell pluripotency and tissue-specific gene regulation, underscoring its broader biological significance. By providing a purified, active protein, recombinant HMGN5 accelerates mechanistic insights into epigenetic regulation and potential clinical applications in precision medicine.
×
