| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ADD3 |
| Uniprot No | Q9UEY8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-706aa |
| 氨基酸序列 | SSDASQGVI TTPPPPSMPH KERYFDRINE NDPEYIRERN MSPDLRQDFN MMEQRKRVTQ ILQSPAFRED LECLIQEQMK KGHNPTGLLA LQQIADYIMA NSFSGFSSPP LSLGMVTPIN DLPGADTSSY VKGEKLTRCK LASLYRLVDL FGWAHLANTY ISVRISKEQD HIIIIPRGLS FSEATASNLV KVNIIGEVVD QGSTNLKIDH TGFSPHAAIY STRPDVKCVI HIHTLATAAV SSMKCGILPI SQESLLLGDV AYYDYQGSLE EQEERIQLQK VLGPSCKVLV LRNHGVVALG ETLEEAFHYI FNVQLACEIQ VQALAGAGGV DNLHVLDFQK YKAFTYTVAA SGGGGVNMGS HQKWKVGEIE FEGLMRTLDN LGYRTGYAYR HPLIREKPRH KSDVEIPATV TAFSFEDDTV PLSPLKYMAQ RQQREKTRWL NSPNTYMKVN VPEESRNGET SPRTKITWMK AEDSSKVSGG TPIKIEDPNQ FVPLNTNPNE VLEKRNKIRE QNRYDLKTAG PQSQLLAGIV VDKPPSTMQF EDDDHGPPAP PNPFSHLTEG ELEEYKRTIE RKQQGLEDAE QELLSDDASS VSQIQSQTQS PQNVPEKLEE NHELFSKSFI SMEVPVMVVN GKDDMHDVED ELAKRVSRLS TSTTIENIEI TIKSPEKIEE VLSPEGSPSK SPSKKKKKFR TPSFLKKNKK KEKVEA |
| 预测分子量 | 79,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ADD3重组蛋白的假设性参考文献示例(请注意，这些文献为虚构内容，实际研究中请通过学术数据库核实)：
1. **《重组ADD3蛋白在细胞骨架重塑中的功能研究》**
- 作者：Zhang Y. et al.
- 摘要：本研究通过大肠杆菌表达系统成功制备了高纯度ADD3重组蛋白，并发现其能通过结合肌动蛋白调控细胞骨架动态，影响上皮细胞的迁移能力。
2. **《ADD3重组蛋白与高血压相关信号通路的相互作用》**
- 作者：Wang L. et al.
- 摘要：利用体外Pull-down实验验证了ADD3重组蛋白与肾小管细胞中钙离子通道蛋白的相互作用，提示其在血压调节中的潜在分子机制。
3. **《结构解析：ADD3重组蛋白的晶体学分析及其磷酸化修饰位点》**
- 作者：Smith J. et al.
- 摘要：通过X射线衍射获得了ADD3重组蛋白的晶体结构，揭示了其C端结构域在磷酸化修饰中的构象变化，为药物靶点设计提供依据。
4. **《ADD3重组蛋白在肝癌细胞凋亡中的双重作用》**
- 作者：Chen H. et al.
- 摘要：研究发现ADD3重组蛋白在低浓度时促进肝癌细胞凋亡，而高浓度可能通过激活AKT通路抑制凋亡，提示其复杂的生物学角色。
**建议**：实际研究中可通过PubMed、Web of Science等平台以“ADD3 recombinant protein”“Adducin gamma function”为关键词检索最新文献。
**Background of ADD3 Recombinant Protein**
ADD3 (Adducin Gamma) recombinant protein is derived from the *ADD3* gene, which encodes the gamma subunit of the adducin family. Adducins are cytoskeletal proteins critical for maintaining membrane integrity and cell-cell adhesion by stabilizing the spectrin-actin network beneath the plasma membrane. The ADD3 protein, along with alpha (ADD1) and beta (ADD2) subunits, forms heterodimeric or heterotetrameric complexes that regulate actin filament capping, synaptic plasticity, and signal transduction.
Structurally, ADD3 contains an N-terminal globular head domain, a neck region, and a C-terminal tail with multiple phosphorylation sites. Its activity is modulated by calcium/calmodulin and protein kinase C (PKC)-mediated phosphorylation, influencing interactions with spectrin, actin, and other cytoskeletal components. Dysregulation of ADD3 has been implicated in pathological conditions, including cancers (e.g., hepatocellular carcinoma, leukemia), neurological disorders, and hypertension. For instance, reduced ADD3 expression correlates with tumor metastasis, while mutations may disrupt erythrocyte membrane stability, contributing to hemolytic disorders.
Recombinant ADD3 protein is typically produced using bacterial (e.g., *E. coli*) or mammalian expression systems, enabling studies on its biochemical properties, post-translational modifications, and interactions. Purified ADD3 recombinant proteins often include tags (e.g., His, GST) for affinity chromatography and detection. These tools facilitate research into adducin’s role in cellular mechanics, disease mechanisms, and therapeutic targeting. For example, ADD3 recombinant proteins are used in *in vitro* binding assays, structural studies, and antibody development, aiding the exploration of cytoskeletal dynamics and potential biomarkers for related diseases.
In summary, ADD3 recombinant protein serves as a vital resource for dissecting adducin-mediated cytoskeletal regulation and its implications in health and disease.
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