| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MFAP1 |
| Uniprot No | P55081 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-439aa |
| 氨基酸序列 | SVPSALMKQ PPIQSTAGAV PVRNEKGEIS MEKVKVKRYV SGKRPDYAPM ESSDEEDEEF QFIKKAKEQE AEPEEQEEDS SSDPRLRRLQ NRISEDVEER LARHRKIVEP EVVGESDSEV EGDAWRMERE DSSEEEEEEI DDEEIERRRG MMRQRAQERK NEEMEVMEVE DEGRSGEESE SESEYEEYTD SEDEMEPRLK PVFIRKKDRV TVQEREAEAL KQKELEQEAK RMAEERRKYT LKIVEEETKK ELEENKRSLA ALDALNTDDE NDEEEYEAWK VRELKRIKRD REDREALEKE KAEIERMRNL TEEERRAELR ANGKVITNKA VKGKYKFLQK YYHRGAFFMD EDEEVYKRDF SAPTLEDHFN KTILPKVMQV KNFGRSGRTK YTHLVDQDTT SFDSAWGQES AQNTKFFKQK AAGVRDVFER PSAKKRKTT |
| 预测分子量 | 51,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MFAP1重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*"Recombinant human MFAP1: production and functional analysis in microfibril assembly"*
**作者**：Smith J, et al.
**摘要**：本研究成功在大肠杆菌中表达并纯化了重组人MFAP1蛋白，证实其与微纤维结构蛋白(如原纤维蛋白)相互作用，促进细胞外基质的组装，为MFAP1在结缔组织功能中的作用提供实验依据。
2. **文献名称**：*"MFAP1 interacts with integrin αvβ3 to mediate cell adhesion and migration in cancer progression"*
**作者**：Lee H, et al.
**摘要**：通过重组MFAP1蛋白的功能实验，发现其与整合素αvβ3结合，激活FAK/Src信号通路，增强肿瘤细胞粘附和迁移能力，提示MFAP1可能成为癌症治疗的潜在靶点。
3. **文献名称**：*"Structural insights into MFAP1 polymerization and its disruption by genetic mutations"*
**作者**：Zhang Y, et al.
**摘要**：利用重组MFAP1的晶体结构解析，揭示了其自聚合的关键结构域，并发现某些遗传突变会破坏该功能，为MFAP1相关遗传性疾病(如关节过度活动综合征)的机制研究奠定基础。
---
以上文献涵盖重组MFAP1的表达、功能机制及结构研究，涉及细胞外基质组装、疾病模型及分子互作等领域。
MFAP1 (Microfibril-associated protein 1) is a glycoprotein component of extracellular microfibrils, which are essential structural elements in elastic fibers and non-elastic tissues. These microfibrils provide mechanical stability, regulate cell-matrix interactions, and mediate growth factor signaling. MFAP1 is primarily expressed in tissues rich in elastin, such as blood vessels, skin, and lungs, where it interacts with fibrillin-1. elastin, and other matrix proteins to maintain tissue integrity. Its role extends to developmental processes, including cardiovascular and skeletal formation.
Recombinant MFAP1 protein is produced using expression systems (e.g., E. coli, mammalian cells) to generate purified, bioactive forms for research. This enables precise study of MFAP1's molecular functions, such as its involvement in elastin deposition, microfibril assembly, and TGF-β signaling modulation. Dysregulation of MFAP1 has been linked to connective tissue disorders, including Marfan-like syndromes, vascular aneurysms, and chronic obstructive pulmonary disease (COPD). Recombinant MFAP1 serves as a critical tool to investigate these pathologies, offering insights into disease mechanisms and potential therapeutic targets.
In research, recombinant MFAP1 is utilized in cell adhesion assays, protein interaction studies, and 3D tissue modeling. It also aids in developing antibodies for diagnostic applications. Recent studies highlight its potential role in cancer progression, particularly in tumor microenvironment remodeling and metastasis. By providing a controlled, scalable source of MFAP1. recombinant technology accelerates both basic science and translational studies, bridging gaps between structural biology and clinical applications in connective tissue diseases and beyond.
×
