| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ACSL6 |
| Uniprot No | Q9UKU0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-697aa |
| 氨基酸序列 | MQTQEILRILRLPELGDLGQFFRSLSATTLVSMGALAAILAYWFTHRPKALQPPCNLLMQSEEVEDSGGARRSVIGSGPQLLTHYYDDARTMYQVFRRGLSISGNGPCLGFRKPKQPYQWLSYQEVADRAEFLGSGLLQHNCKACTDQFIGVFAQNRPEWIIVELACYTYSMVVVPLYDTLGPGAIRYIINTADISTVIVDKPQKAVLLLEHVERKETPGLKLIILMDPFEEALKERGQKCGVVIKSMQAVEDCGQENHQAPVPPQPDDLSIVCFTSGTTGNPKGAMLTHGNVVADFSGFLKVTEKVIFPRQDDVLISFLPLAHMFERVIQSVVYCHGGRVGFFQGDIRLLSDDMKALCPTIFPVVPRLLNRMYDKIFSQANTPLKRWLLEFAAKRKQAEVRSGIIRNDSIWDELFFNKIQASLGGCVRMIVTGAAPASPTVLGFLRAALGCQVYEGYGQTECTAGCTFTTPGDWTSGHVGAPLPCNHIKLVDVEELNYWACKGEGEICVRGPNVFKGYLKDPDRTKEALDSDGWLHTGDIGKWLPAGTLKIIDRKKHIFKLAQGEYVAPEKIENIYIRSQPVAQIYVHGDSLKAFLVGIVVPDPEVMPSWAQKRGIEGTYADLCTNKDLKKAILEDMVRLGKESGLHSFEQVKAIHIHSDMFSVQNGLLTPTLKAKRPELREYFKKQIEELYSISM |
| 预测分子量 | 77,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ACSL6重组蛋白的3篇参考文献及其简要摘要：
1. **文献名称**：*ACSL6 regulates lipid metabolism in glioblastoma and modulates tumor cell proliferation*
**作者**：Zhang, Y. et al.
**摘要**：该研究通过表达ACSL6重组蛋白，发现其能够促进神经胶质瘤细胞中长链脂肪酸的活化，进而影响肿瘤细胞的脂质代谢和增殖能力，揭示了ACSL6在癌症中的潜在治疗靶点作用。
2. **文献名称**：*Recombinant ACSL6 characterization and its role in myeloid differentiation*
**作者**：Smith, J.P. et al.
**摘要**：作者利用重组ACSL6蛋白进行体外酶活性分析，发现其特异性激活C20:4脂肪酸，并证明其在髓系细胞分化过程中通过调控脂质信号通路发挥作用。
3. **文献名称**：*Structural insights into ACSL6 substrate specificity through recombinant protein crystallization*
**作者**：Lee, H. & Kim, S.
**摘要**：研究通过重组ACSL6蛋白的结晶和结构解析，揭示了其底物结合口袋的关键氨基酸残基，阐明了其对不同链长脂肪酸的选择性催化机制。
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注：上述文献为示例，实际引用时需根据具体论文核实信息。若需查找真实文献，建议通过PubMed或SciHub等平台搜索关键词“ACSL6 recombinant”“ACSL6 purification”等。
ACSL6 (Acyl-CoA Synthesase Long-Chain Family Member 6) is a key enzyme in lipid metabolism, primarily catalyzing the activation of long-chain fatty acids into acyl-CoA esters. This reaction is critical for fatty acid oxidation, membrane synthesis, and lipid signaling. The ACSL family consists of multiple isoforms with distinct tissue distributions and substrate preferences. ACSL6 is notably expressed in the brain, bone marrow, heart, and adrenal glands, and has been implicated in both normal physiological processes and disease pathways, including cancer, neurological disorders, and metabolic syndromes.
Recombinant ACSL6 protein is engineered through molecular cloning techniques, often using expression systems like *E. coli*, yeast, or mammalian cells. Its production enables detailed biochemical studies, such as enzymatic activity assays, substrate specificity profiling, and interaction analyses with regulatory proteins or inhibitors. Research has highlighted ACSL6’s role in lipid remodeling of cell membranes, particularly in neurons and hematopoietic cells. Dysregulation of ACSL6 is linked to acute myeloid leukemia (AML), where chromosomal rearrangements involving the *ACSL6* gene may drive oncogenesis. Additionally, altered ACSL6 expression has been observed in neurodevelopmental disorders, suggesting its importance in brain lipid homeostasis.
The recombinant protein also serves as a tool for drug discovery, aiding in the identification of compounds that modulate fatty acid metabolism. Structural studies using purified ACSL6 help elucidate mechanisms of substrate binding and catalysis, offering insights for targeted therapies. Despite progress, questions remain about isoform-specific functions and tissue-specific regulatory networks, driving ongoing research into ACSL6’s multifaceted roles in health and disease.
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