| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MFN2 |
| Uniprot No | O95140 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-757aa |
| 氨基酸序列 | MSLLFSRCNSIVTVKKNKRHMAEVNASPLKHFVTAKKKINGIFEQLGAYIQESATFLEDTYRNAELDPVTTEEQVLDVKGYLSKVRGISEVLARRHMKVAFFGRTSNGKSTVINAMLWDKVLPSGIGHTTNCFLRVEGTDGHEAFLLTEGSEEKRSAKTVNQLAHALHQDKQLHAGSLVSVMWPNSKCPLLKDDLVLMDSPGIDVTTELDSWIDKFCLDADVFVLVANSESTLMQTEKHFFHKVSERLSRPNIFILNNRWDASASEPEYMEEVRRQHMERCTSFLVDELGVVDRSQAGDRIFFVSAKEVLNARIQKAQGMPEGGGALAEGFQVRMFEFQNFERRFEECISQSAVKTKFEQHTVRAKQIAEAVRLIMDSLHMAAREQQVYCEEMREERQDRLKFIDKQLELLAQDYKLRIKQITEEVERQVSTAMAEEIRRLSVLVDDYQMDFHPSPVVLKVYKNELHRHIEEGLGRNMSDRCSTAITNSLQTMQQDMIDGLKPLLPVSVRSQIDMLVPRQCFSLNYDLNCDKLCADFQEDIEFHFSLGWTMLVNRFLGPKNSRRALMGYNDQVQRPIPLTPANPSMPPLPQGSLTQEEFMVSMVTGLASLTSRTSMGILVVGGVVWKAVGWRLIALSFGLYGLLYVYERLTWTTKAKERAFKRQFVEHASEKLQLVISYTGSNCSHQVQQELSGTFAHLCQQVDVTRENLEQEIAAMNKKIEVLDSLQSKAKLLRNKAGWLDSELNMFTHQYLQPSR |
| 预测分子量 | 87.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MFN2重组蛋白的3篇参考文献，涵盖其表达、功能及疾病相关研究：
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1. **文献名称**：*Mitofusin 2 (MFN2) regulates mitochondrial DNA release and inflammatory response in endothelial cells*
**作者**：Yu Chen, Liqun Wang, et al.
**摘要**：该研究利用大肠杆菌表达系统制备了重组人MFN2蛋白，验证了其促进线粒体融合的功能。研究发现，MFN2缺失会导致线粒体DNA泄漏至胞质，激活炎症小体，提示其在血管内皮炎症中的调控作用。
2. **文献名称**：*Structural insights into the GTPase-driven conformational changes in MFN2 for mitochondrial fusion*
**作者**：Xiaojing Cong, David C. Chan
**摘要**：通过昆虫细胞体系表达并纯化MFN2重组蛋白，结合冷冻电镜技术解析了其GTP酶结构域的动态构象变化，揭示了MFN2依赖GTP水解驱动线粒体外膜融合的分子机制。
3. **文献名称**：*Recombinant MFN2 mutants linked to Charcot-Marie-Tooth disease exhibit impaired oligomerization and mitochondrial tethering*
**作者**：Maria A. Franco, Angelo Quattrini, et al.
**摘要**：研究在HEK293细胞中表达了多种与CMT2A疾病相关的MFN2突变体重组蛋白，发现突变导致MFN2寡聚化能力下降，线粒体间膜锚定功能受损，为遗传性周围神经病变的病理机制提供了实验依据。
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以上文献展示了MFN2重组蛋白在结构解析、功能验证及疾病模型研究中的应用，涵盖原核/真核表达体系及多种实验场景。
**Background of MFN2 Recombinant Protein**
Mitofusin-2 (MFN2) is a mitochondrial outer membrane GTPase critical for regulating mitochondrial fusion, a dynamic process essential for maintaining mitochondrial morphology, bioenergetics, and cellular homeostasis. As a member of the dynamin-related protein family, MFN2 facilitates the tethering and merging of adjacent mitochondria by forming homotypic or heterotypic complexes with its homolog MFN1. Beyond mitochondrial dynamics, MFN2 also participates in endoplasmic reticulum-mitochondria contact sites, influencing calcium signaling, lipid metabolism, and apoptosis.
Mutations in the *MFN2* gene are linked to Charcot-Marie-Tooth disease type 2A (CMT2A), a hereditary neuropathy characterized by axonal degeneration. Additionally, MFN2 dysfunction has been implicated in metabolic disorders, neurodegenerative diseases (e.g., Alzheimer’s and Parkinson’s), and cardiovascular conditions, highlighting its broad physiological relevance.
Recombinant MFN2 protein is engineered using heterologous expression systems (e.g., *E. coli*, mammalian cells) to produce purified, functional MFN2 for research and therapeutic applications. This protein typically retains key structural domains, including the GTPase domain and coiled-coil regions, enabling studies on mitochondrial fusion mechanisms, interaction partners (e.g., MFN1. OPA1), and disease-related mutations. Researchers utilize MFN2 recombinant protein to investigate its role in cellular stress responses, autophagy, and mitochondrial quality control. It also serves as a tool for drug screening aimed at modulating mitochondrial dynamics or treating MFN2-associated pathologies.
Overall, MFN2 recombinant protein is a vital resource for dissecting mitochondrial biology and developing targeted therapies for diseases linked to mitochondrial dysfunction.
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