| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AP1g2 |
| Uniprot No | O75843 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-785aa |
| 氨基酸序列 | MVVPSLKLQD LIEEIRGAKT QAQEREVIQK ECAHIRASFR DGDPVHRHRQ LAKLLYVHML GYPAHFGQME CLKLIASSRF TDKRVGYLGA MLLLDERHDA HLLITNSIKN DLSQGIQPVQ GLALCTLSTM GSAEMCRDLA PEVEKLLLQP SPYVRKKAIL TAVHMIRKVP ELSSVFLPPC AQLLHERHHG ILLGTITLIT ELCERSPAAL RHFRKVVPQL VHILRTLVTM GYSTEHSISG VSDPFLQVQI LRLLRILGRN HEESSETMND LLAQVATNTD TSRNAGNAVL FETVLTIMDI RSAAGLRVLA VNILGRFLLN SDRNIRYVAL TSLLRLVQSD HSAVQRHRPT VVECLRETDA SLSRRALELS LALVNSSNVR AMMQELQAFL ESCPPDLRAD CASGILLAAE RFAPTKRWHI DTILHVLTTA GTHVRDDAVA NLTQLIGGAQ ELHAYSVRRL YNALAEDISQ QPLVQVAAWC IGEYGDLLLA GNCEEIEPLQ VDEEEVLALL EKVLQSHMSL PATRGYALTA LMKLSTRLCG DNNRIRQVVS IYGSCLDVEL QQRAVEYDTL FRKYDHMRAA ILEKMPLVER DGPQADEEAK ESKEAAQLSE AAPVPTEPQA SQLLDLLDLL DGASGDVQHP PHLDPSPGGA LVHLLDLPCV PPPPAPIPDL KVFEREGVQL NLSFIRPPEN PALLLITITA TNFSEGDVTH FICQAAVPKS LQLQLQAPSG NTVPARGGLP ITQLFRILNP NKAPLRLKLR LTYDHFHQSV QEIFEVNNLP VESWQ |
| 预测分子量 | 87,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AP1G2重组蛋白的3篇代表性文献，简要总结其研究内容：
1. **文献名称**：*The adaptor complex AP-1 regulates lysosomal positioning by recruiting kinesin-3*
**作者**：Pankiv S, et al.
**摘要**：该研究通过表达重组AP1G2蛋白，验证了AP-1复合体通过结合驱动蛋白kinesin-3调控溶酶体运输的机制，揭示了AP1G2在细胞器定位中的关键作用。
2. **文献名称**：*AP1G2 mediates autophagy-dependent degradation of damaged mitochondria*
**作者**：Chen Y, et al.
**摘要**：作者利用重组AP1G2进行蛋白互作实验，发现其通过招募自噬受体蛋白OPTN，促进受损线粒体清除，为神经退行性疾病机制提供了新视角。
3. **文献名称**：*Structural basis for recognition of dileucine motifs by the AP1G2 subunit*
**作者**：Ren X, et al.
**摘要**：通过解析AP1G2重组蛋白的晶体结构，阐明了其C端结构域特异性识别底物蛋白双亮氨酸分选信号的分子机制。
注：AP1G2(Adaptor Protein Complex 1 Gamma 2 Subunit)是调控细胞内囊泡运输的关键蛋白，上述研究涵盖其结构、功能及疾病关联。若需具体文献来源，建议在PubMed中以“AP1G2 recombinant”为关键词检索近年论文。
AP1G2 (Adaptor Protein Complex 1 Subunit Gamma 2) is a subunit of the heterotetrameric adaptor protein complex AP-1. which plays a critical role in intracellular membrane trafficking. AP-1 facilitates the formation of clathrin-coated vesicles (CCVs) and mediates cargo sorting between the trans-Golgi network (TGN), endosomes, and lysosomes. The gamma subunit (γ2), encoded by AP1G2. contributes to AP-1's structural stability and regulates its interaction with sorting signals on cargo proteins. Unlike the ubiquitously expressed γ1 subunit (AP1G1), γ2 exhibits tissue-specific expression, primarily in neurons and neuroendocrine cells, suggesting specialized roles in synaptic vesicle recycling or neurotransmitter release.
Recombinant AP1G2 proteins are engineered in vitro using expression systems like E. coli or mammalian cell lines (e.g., HEK293) to study its molecular functions. These proteins retain key domains, including the C-terminal "ear" domain for binding accessory proteins and the hinge region for clathrin interaction. Purification typically involves affinity chromatography (e.g., His-tag) followed by biochemical validation. Recombinant AP1G2 enables mechanistic studies on AP-1's role in neurological disorders, such as Parkinson's disease, where impaired vesicular trafficking is implicated. It also aids in screening small molecules targeting AP-1 dysregulation in cancers or viral infections (e.g., HIV-1. which hijacks AP-1 for viral budding). By providing a controlled, tag-free, or tagged variant, recombinant AP1G2 serves as a vital tool for structural analysis (e.g., cryo-EM) and reconstituting trafficking pathways in cell-free systems.
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