| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPLP1 |
| Uniprot No | P05386 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-114aa |
| 氨基酸序列 | ASVSELACIYSALILHDDEVTVTEDKINALIKAAGVNVEPFWPGLFAKALANVNIGSLICNVGAGGPAPAAGAAPAGGPAPSTAAAPAEEKKVEAKKEESEESDDDMGFGLFD |
| 预测分子量 | 27.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPLP1重组蛋白的3篇示例参考文献(内容为模拟，请根据实际文献调整)：
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1. **文献名称**：*Structural characterization of recombinant human RPLP1 and its role in ribosome assembly*
**作者**：Smith J, et al.
**摘要**：本研究通过大肠杆菌表达系统纯化重组人源RPLP1蛋白，并利用X射线晶体学解析其三维结构，揭示了其与核糖体其他亚基的相互作用位点，为理解核糖体组装机制提供依据。
2. **文献名称**：*RPLP1 overexpression modulates translation in cancer cells via mTOR signaling*
**作者**：Chen L, et al.
**摘要**：通过重组RPLP1蛋白在乳腺癌细胞中的过表达实验，发现其通过激活mTOR通路增强核糖体翻译效率，提示RPLP1在肿瘤进展中的潜在调控作用。
3. **文献名称**：*Development of a high-yield RPLP1 recombinant protein production system in Pichia pastoris*
**作者**：García-Ruiz E, et al.
**摘要**：优化毕赤酵母表达系统实现RPLP1的高效分泌表达，纯化蛋白具有天然活性，为大规模制备用于抗体开发或功能研究奠定基础。
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**提示**：以上为示例文献，实际文献需通过PubMed、Google Scholar等平台检索关键词“RPLP1 recombinant protein”“RPLP1 structural analysis”等获取，并注意选择近5年高影响力期刊研究。
**Background of RPLP1 Recombinant Protein**
Ribosomal Protein Lateral Stalk Subunit P1 (RPLP1) is a conserved acidic phosphoprotein component of the 60S ribosomal subunit, playing a critical role in translation elongation by stabilizing ribosomal structure and facilitating interactions with elongation factors. As part of the ribosomal P-complex, RPLP1 forms a heterodimer with RPLP2. anchoring the GTPase-associated center to regulate protein synthesis. It is ubiquitously expressed across tissues, often serving as a housekeeping gene in molecular studies due to its stable expression.
The recombinant RPLP1 protein is engineered using prokaryotic or eukaryotic expression systems, enabling controlled production for functional and structural studies. Its recombinant form typically includes affinity tags (e.g., His-tag) for purification and detection. Researchers utilize this protein to investigate ribosome assembly, translational regulation, and interactions with other ribosomal components or cellular factors.
Beyond basic research, RPLP1 has clinical relevance. Overexpression of RPLP1 has been linked to cancer progression (e.g., breast, colorectal cancers), where it may enhance ribosome biogenesis to support tumor growth. Additionally, RPLP1 is implicated in autoimmune diseases, acting as an autoantigen targeted by antibodies in conditions like systemic lupus erythematosus. Recombinant RPLP1 aids in developing diagnostic tools or therapeutic strategies by serving as an antigen or target for drug screening.
Studies also explore its role in viral infections, as some pathogens exploit ribosomal proteins for replication. Overall, RPLP1 recombinant protein is a vital tool for dissecting ribosome biology and its connections to disease mechanisms.
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