| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ENOX1 |
| Uniprot No | Q8TC92 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-643aa |
| 氨基酸序列 | MVDAGGVENITQLPQELPQMMAAAADGLGSIAIDTTQLNMSVTDPTAWAT AMNNLGMVPVGLPGQQLVSDSICVPGFDPSLNMMTGITPINPMIPGLGLV PPPPPTEVAVVKEIIHCKSCTLFPQNPNLPPPSTRERPPGCKTVFVGGLP ENATEEIIQEVFEQCGDITAIRKSKKNFCHIRFAEEFMVDKAIYLSGYRM RLGSSTDKKDSGRLHVDFAQARDDFYEWECKQRMRAREERHRRKLEEDRL RPPSPPAIMHYSEHEAALLAEKLKDDSKFSEAITVLLSWIERGEVNRRSA NQFYSMVQSANSHVRRLMNEKATHEQEMEEAKENFKNALTGILTQFEQIV AVFNASTRQKAWDHFSKAQRKNIDIWRKHSEELRNAQSEQLMGIRREEEM EMSDDENCDSPTKKMRVDESALAAQAYALKEENDSLRWQLDAYRNEVELL KQEKEQLFRTEENLTKDQQLQFLQQTMQGMQQQLLTIQEELNNKKSELEQ AKEEQSHTQALLKVLQEQLKGTKELVETNGHSHEDSNEINVLTVALVNQD RENNIEKRSQGLKSEKEALLIGIISTFLHVHPFGANIEYLWSYMQQLDSK ISANEIEMLLMRLPRMFKQEFTGVGATLEKRWKLCAFEGIKTT |
| 预测分子量 | 77 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ENOX1重组蛋白的模拟参考文献(仅供参考，建议通过学术数据库验证具体信息)：
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1. **文献名称**: *"Expression and Functional Characterization of Recombinant Human ENOX1 Protein in E. coli"*
**作者**: Zhang Y, et al.
**摘要**: 该研究成功在大肠杆菌中表达并纯化重组ENOX1蛋白，验证其核苷酸酶活性，发现其在调控细胞外ATP/ADP代谢中的关键作用，并证实其与炎症信号通路的关联。
2. **文献名称**: *"Structural Insights into ENOX1 Catalytic Mechanism via Crystallography of Recombinant Protein"*
**作者**: Lee S, et al.
**摘要**: 通过X射线晶体学解析重组ENOX1蛋白的三维结构，揭示了其底物结合位点和催化活性中心的关键氨基酸残基，为设计靶向ENOX1的小分子抑制剂提供结构基础。
3. **文献名称**: *"ENOX1 Recombinant Protein Attenuates Tumor Growth in a Mouse Xenograft Model"*
**作者**: Chen H, et al.
**摘要**: 利用哺乳动物细胞表达系统生产功能性ENOX1重组蛋白，发现其通过降解肿瘤微环境中的ATP抑制癌细胞增殖，在结肠癌小鼠模型中显著减缓肿瘤进展。
4. **文献名称**: *"Development of a High-Throughput Assay for ENOX1 Activity Using Recombinant Protein"*
**作者**: Müller R, et al.
**摘要**: 开发基于重组ENOX1蛋白的高通量筛选平台，用于筛选潜在酶活性调节剂，发现多种天然化合物可显著抑制ENOX1活性，提示其在代谢疾病治疗中的应用潜力。
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**注意**：以上文献为模拟生成，实际研究需通过PubMed、Web of Science等平台检索。建议结合关键词“ENOX1 recombinant protein”或“ENOX1 expression”进一步验证。
ENOX1 (Ecto-NOX disulfide-thiol exchanger 1) is a member of the NADH oxidase family, implicated in cellular redox signaling and growth regulation. Initially identified as a cell surface protein, it exhibits oxidoreductase activity, cycling between dithiol and disulfide states to facilitate electron transfer. ENOX1 is thought to play a role in cell proliferation, differentiation, and apoptosis, potentially linking extracellular redox dynamics to intracellular signaling pathways. Structurally, it contains conserved motifs like the copper-binding domain and transmembrane regions, though its precise catalytic mechanism remains under investigation.
Recombinant ENOX1 protein is engineered for in vitro studies to elucidate its biological functions and interactions. Produced via heterologous expression systems (e.g., bacterial or mammalian cells), it enables biochemical characterization, including enzymatic assays, substrate specificity profiling, and inhibitor screening. Its recombinant form is critical for cancer research, as ENOX1 overexpression has been observed in certain malignancies, suggesting its role as a biomarker or therapeutic target. Additionally, studies explore its involvement in neurodegenerative diseases, where oxidative stress pathways are dysregulated. The protein’s extracellular orientation also makes it a candidate for antibody-based therapies. Despite progress, challenges persist in resolving its full-length structure and tissue-specific regulatory mechanisms, driving ongoing research into its physiological and pathological relevance.
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