| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OSBP2 |
| Uniprot No | Q969R2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-916aa |
| 氨基酸序列 | MGKAAAPSRGGGCGGRSRGLSSLFTVVPCLSCHTAAPGMSASTSGSGPEPKPQPQPVPEPERGPLSEQVSEAVSEAVPRSEPVSETTSEPEPGAGQPSELLQGSRPGSESSSGVGAGPFTKAASEPLSRAVGSATFLRPESGSLPALKPLPLLRPGQAKTPLGVPMSGTGTTSSAPLALLPLDSFEGWLLKWTNYLKGYQRRWFVLGNGLLSYYRNQGEMAHTCRGTINLSTAHIDTEDSCGILLTSGARSYHLKASSEVDRQQWITALELAKAKAVRVMNTHSDDSGDDDEATTPADKSELHHTLKNLSLKLDDLSTCNDLIAKHGAALQRSLTELDGLKIPSESGEKLKVVNERATLFRITSNAMINACRDFLELAEIHSRKWQRALQYEQEQRVHLEETIEQLAKQHNSLERAFHSAPGRPANPSKSFIEGSLLTPKGEDSEEDEDTEYFDAMEDSTSFITVITEAKEDSRKAEGSTGTSSVDWSSADNVLDGASLVPKGSSKVKRRVRIPNKPNYSLNLWSIMKNCIGRELSRIPMPVNFNEPLSMLQRLTEDLEYHHLLDKAVHCTSSVEQMCLVAAFSVSSYSTTVHRIAKPFNPMLGETFELDRLDDMGLRSLCEQVSHHPPSAAHYVFSKHGWSLWQEITISSKFRGKYISIMPLGAIHLEFQASGNHYVWRKSTSTVHNIIVGKLWIDQSGDIEIVNHKTNDRCQLKFLPYSYFSKEAARKVTGVVSDSQGKAHYVLSGSWDEQMECSKVMHSSPSSPSSDGKQKTVYQTLSAKLLWKKYPLPENAENMYYFSELALTLNEHEEGVAPTDSRLRPDQRLMEKGRWDEANTEKQRLEEKQRLSRRRRLEACGPGSSCSSEEEKEADAYTPLWFEKRLDPLTGEMACVYKGGYWEAKEKQDWHMCPNIF |
| 预测分子量 | 101,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于OSBP2重组蛋白的3篇参考文献的简要信息：
1. **文献名称**: "Expression and Functional Characterization of Recombinant OSBP2 in Cholesterol Transport"
**作者**: Takahashi, M. et al.
**摘要**: 该研究成功在哺乳动物细胞中表达了重组人源OSBP2蛋白，并验证其与氧化固醇(如25-羟基胆固醇)的结合能力。实验表明，OSBP2通过PH结构域靶向高尔基体膜，并依赖其FFAT基序与内质网膜蛋白VAPA/B相互作用，调控细胞内胆固醇的分布和运输。
2. **文献名称**: "Structural Insights into OSBP2-Mediated Lipid Transfer by Cryo-EM"
**作者**: Li, J. & Zhou, Y.
**摘要**: 本研究利用冷冻电镜技术解析了重组OSBP2蛋白的三维结构，揭示了其脂质结合口袋的构象变化。作者发现OSBP2通过交替接触高尔基体和内质网膜，以“穿梭机制”转运固醇类脂质，并提出其功能缺陷可能与神经退行性疾病相关。
3. **文献名称**: "OSBP2 Regulates Autophagy via Interaction with LC3 in Recombinant Protein Models"
**作者**: Wang, Q. et al.
**摘要**: 通过在大肠杆菌中表达并纯化OSBP2的重组片段，研究发现OSBP2的ORD结构域可直接结合自噬标记蛋白LC3.并在饥饿条件下促进自噬小体形成。该研究为OSBP2在细胞应激反应中的新功能提供了证据。
注：上述文献为模拟示例，实际研究中请通过学术数据库检索具体论文。OSBP2的研究相对较少，建议结合关键词“OSBP2 recombinant”“oxysterol-binding protein 2 expression”或“OSBP2 lipid transport”进一步筛选。
**Background of OSBP2 Recombinant Protein**
The Oxysterol-Binding Protein 2 (OSBP2), also known as ORP2 (OSBP-related protein 2), belongs to the OSBP/ORP family of lipid-binding proteins involved in intracellular lipid transport, signaling, and membrane dynamics. OSBP2 is encoded by the *OSBPL2* gene in humans and shares structural homology with other family members, featuring an N-terminal pleckstrin homology (PH) domain for membrane targeting and a C-terminal oxysterol-binding (OSBP) domain for lipid recognition. Unlike its well-studied relative OSBP1. which primarily mediates cholesterol and phosphatidylinositol 4-phosphate (PI4P) exchange at membrane contact sites, OSBP2 exhibits distinct lipid preferences and subcellular localization, suggesting specialized roles in lipid homeostasis.
Recombinant OSBP2 protein is engineered using expression systems (e.g., bacterial, mammalian, or insect cells) to produce purified, functional protein for research. Its production often involves tagging (e.g., His-tag, GST) for affinity purification and detection. Studies suggest OSBP2 regulates cholesterol trafficking, particularly in late endosomes/lysosomes, and interacts with vesicular transport machinery, influencing cellular lipid distribution and signaling. Dysregulation of OSBP2 is implicated in metabolic disorders, neurodegenerative diseases, and cancer progression, making it a target for therapeutic exploration.
Research applications include *in vitro* lipid-binding assays, structural studies to map functional domains, and cell-based experiments to elucidate its role in lipid metabolism. Challenges in working with recombinant OSBP2 include maintaining its lipid-binding activity post-purification and addressing its susceptibility to aggregation. Overall, OSBP2 recombinant protein serves as a critical tool for unraveling the molecular mechanisms of lipid-mediated cellular processes and disease pathogenesis.
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