| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AMPD3 |
| Uniprot No | Q01432 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-767aa |
| 氨基酸序列 | MPRQFPKLNISEVDEQVRLLAEKVFAKVLREEDSKDALSLFTVPEDCPIGQKEAKERELQKELAEQKSVETAKRKKSFKMIRSQSLSLQMPPQQDWKGPPAASPAMSPTTPVVTGATSLPTPAPYAMPEFQRVTISGDYCAGITLEDYEQAAKSLAKALMIREKYARLAYHRFPRITSQYLGHPRADTAPPEEGLPDFHPPPLPQEDPYCLDDAPPNLDYLVHMQGGILFVYDNKKMLEHQEPHSLPYPDLETYTVDMSHILALITDGPTKTYCHRRLNFLESKFSLHEMLNEMSEFKELKSNPHRDFYNVRKVDTHIHAAACMNQKHLLRFIKHTYQTEPDRTVAEKRGRKITLRQVFDGLHMDPYDLTVDSLDVHAGRQTFHRFDKFNSKYNPVGASELRDLYLKTENYLGGEYFARMVKEVARELEESKYQYSEPRLSIYGRSPEEWPNLAYWFIQHKVYSPNMRWIIQVPRIYDIFRSKKLLPNFGKMLENIFLPLFKATINPQDHRELHLFLKYVTGFDSVDDESKHSDHMFSDKSPNPDVWTSEQNPPYSYYLYYMYANIMVLNNLRRERGLSTFLFRPHCGEAGSITHLVSAFLTADNISHGLLLKKSPVLQYLYYLAQIPIAMSPLSNNSLFLEYSKNPLREFLHKGLHVSLSTDDPMQFHYTKEALMEEYAIAAQVWKLSTCDLCEIARNSVLQSGLSHQEKQKFLGQNYYKEGPEGNDIRKTNVAQIRMAFRYETLCNELSFLSDAMKSEEITALTN |
| 预测分子量 | 88,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AMPD3重组蛋白的参考文献及摘要概括：
1. **文献名称**：*"Recombinant expression and functional characterization of human AMPD3: insights into its role in erythrocyte metabolism"*
**作者**：Zhang Y, et al.
**摘要**：研究报道了人源AMPD3基因在大肠杆菌中的重组表达与纯化，证实其催化AMP脱氨的活性，并揭示其在红细胞能量代谢中的潜在调控作用。
2. **文献名称**：*"Production and characterization of a stable AMPD3 recombinant protein for enzymatic deficiency studies"*
**作者**：Martinez R, et al.
**摘要**：通过哺乳动物细胞系统表达AMPD3重组蛋白，优化纯化工艺，验证其酶动力学参数，为AMPD3缺乏症的研究提供可靠工具。
3. **文献名称**：*"Structural and functional analysis of AMPD3 mutations using recombinant protein models"*
**作者**：Kim H, et al.
**摘要**：利用昆虫细胞表达系统获得AMPD3突变体重组蛋白，结合晶体结构解析与功能实验，阐明致病突变对酶活性及稳定性的影响。
4. **文献名称**：*"AMPD3 recombinant protein rescues metabolic dysfunction in CRISPR-engineered cellular models"*
**作者**：Chen L, et al.
**摘要**：在AMPD3敲除细胞系中，外源性添加重组AMPD3蛋白可恢复AMP代谢通路异常，提示其在治疗相关代谢疾病中的潜在应用价值。
(注：以上文献为示例性内容，实际引用时需核对真实数据库及原文信息。)
AMPD3 (adenosine monophosphate deaminase 3) is a key enzyme in purine metabolism, catalyzing the conversion of adenosine monophosphate (AMP) to inosine monophosphate (IMP). This reaction plays a critical role in maintaining cellular energy balance and nucleotide pool regulation. The AMPD3 isoform is predominantly expressed in erythrocytes, lymphoid tissues, and specific epithelial cells, distinguishing it from other AMPD family members (AMPD1. AMPD2) in terms of tissue distribution and regulatory mechanisms. Its activity influences diverse physiological processes, including ATP homeostasis, erythrocyte oxygen transport efficiency, and immune cell function.
Recombinant AMPD3 protein is engineered through molecular cloning, typically expressed in bacterial (e.g., *E. coli*) or eukaryotic systems (e.g., mammalian cell lines) to ensure proper folding and post-translational modifications. This engineered protein retains enzymatic activity and is widely used to study AMPD3's biochemical properties, substrate specificity, and interaction with potential inhibitors or modulators. Researchers employ it to investigate AMPD3's role in pathological conditions such as anemia, where erythrocyte AMPD3 deficiency correlates with reduced ATP catabolism, and in cancer metabolism, where altered purine pathways influence tumor progression.
Current studies focus on AMPD3's regulatory mechanisms, particularly its tissue-specific expression patterns and phosphorylation-mediated activity modulation. The recombinant protein serves as a vital tool for structural biology efforts, including X-ray crystallography and cryo-EM, to resolve its 3D architecture and guide drug design. Challenges persist in fully elucidating its pathophysiological significance, particularly in immune regulation and metabolic disorders, driving ongoing research using recombinant AMPD3 to explore therapeutic targets and diagnostic biomarkers.
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