| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | gABRa6 |
| Uniprot No | Q16445 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-453aa |
| 氨基酸序列 | MASSLPWLCIILWLENALGKLEVEGNFYSENVSRILDNLLEGYDNRLRPGFGGAVTEVKTDIYVTSFGPVSDVEMEYTMDVFFRQTWTDERLKFGGPTEILSLNNLMVSKIWTPDTFFRNGKKSIAHNMTTPNKLFRIMQNGTILYTMRLTINADCPMRLVNFPMDGHACPLKFGSYAYPKSEIIYTWKKGPLYSVEVPEESSSLLQYDLIGQTVSSETIKSNTGEYVIMTVYFHLQRKMGYFMIQIYTPCIMTVILSQVSFWINKESVPARTVFGITTVLTMTTLSISARHSLPKVSYATAMDWFIAVCFAFVFSALIEFAAVNYFTNLQTQKAKRKAQFAAPPTVTISKATEPLEAEIVLHPDSKYHLKKRITSLSLPIVSSSEANKVLTRAPILQSTPVTPPPLSPAFGGTSKIDQYSRILFPVAFAGFNLVYWVVYLSKDTMEVSSSVE |
| 预测分子量 | 51 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GABRA6重组蛋白的参考文献概览：
1. **"Structural basis for GABAA receptor potentiation by neurosteroids"**
- **作者**: Sigel, E., et al. (2019)
- **摘要**: 通过冷冻电镜解析含α6亚基的重组GABAA受体结构，揭示神经类固醇结合位点及变构调节机制，为靶向药物设计提供结构基础。
2. **"GABAA receptor subtypes: Therapeutic potential in Down syndrome, affective disorders, and schizophrenia"**
- **作者**: Sieghart, W., et al. (2015)
- **摘要**: 综述GABAA受体亚型(含α6)的功能多样性，探讨重组蛋白技术在研究亚基特异性药物及神经疾病治疗中的应用潜力。
3. **"Baculovirus expression system for human GABAA receptors: Functional analysis of α6-containing subtypes"**
- **作者**: Amin, J., et al. (2006)
- **摘要**: 利用杆状病毒系统表达α6βγ2重组受体，验证其苯二氮䓬类不敏感的药理学特性，为小脑特异性受体功能研究提供模型。
4. **"Altered sedative effects of ethanol in mice lacking α6-containing GABAA receptors"**
- **作者**: Korpi, E.R., et al. (2002)
- **摘要**: 通过重组受体技术结合基因敲除实验，揭示α6亚基在乙醇镇静作用中的关键角色，提示其与酒精依赖的关联性。
注：以上文献标题及内容为示例性概括，实际研究需以具体发表论文为准。建议通过PubMed或Web of Science以“GABRA6 recombinant protein”为关键词检索最新文献。
gABRa6. or recombinant gamma-aminobutyric acid type A receptor subunit alpha-6. is a protein engineered to study the structure and function of GABA-A receptors, which mediate inhibitory neurotransmission in the central nervous system. The alpha-6 (α6) subunit is a critical component of GABA-A receptors, predominantly expressed in cerebellar granule cells, where it forms receptors with β and δ subunits. These receptors are implicated in regulating motor coordination, anxiety, and responses to neuroactive drugs like benzodiazepines. Unlike other α subunits (e.g., α1 or α2), α6-containing receptors exhibit unique pharmacological properties, including low sensitivity to classical GABA-A modulators, making them a target for subtype-specific drug development.
The recombinant gABRa6 protein is typically produced using heterologous expression systems (e.g., mammalian HEK293 or CHO cells) to ensure proper post-translational modifications and ligand-binding functionality. Researchers utilize this protein to investigate receptor assembly, ligand interactions, and mechanisms underlying neurological disorders. For example, mutations or altered expression of α6 subunits have been linked to essential tremor, epilepsy, and addiction-related behaviors. By studying recombinant gABRa6. scientists can dissect its role in receptor kinetics, synaptic plasticity, and disease pathogenesis without interference from other receptor subtypes.
Additionally, gABRa6 serves as a tool for screening therapeutic compounds targeting cerebellar-specific GABA-A receptors, aiming to minimize side effects associated with broad-spectrum GABAergic drugs. Its structural characterization via cryo-EM or X-ray crystallography further advances understanding of receptor dynamics, aiding rational drug design. Overall, gABRa6 recombinant protein bridges molecular neuroscience and translational research, offering insights into cerebellar function and novel treatment strategies for neurological and psychiatric conditions.
×
