| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HSPF4 |
| Uniprot No | P31689 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-394aa |
| 氨基酸序列 | MVKETTYYDVLGVKPNATQEELKKAYRKLALKYHPDKNPNEGEKFKQISQAYEVLSDAKKRELYDKGGEQAIKEGGAGGGFGSPMDIFDMFFGGGGRMQRERRGKNVVHQLSVTLEDLYNGATRKLALQKNVICDKCEGRGGKKGAVECCPNCRGTGMQIRIHQIGPGMVQQIQSVCMECQGHGERISPKDRCKSCNGRKIVREKKILEVHIDKGMKDGQKITFHGEGDQEPGLEPGDIIIVLDQKDHAVFTRRGEDLFMCMDIQLVEALCGFQKPISTLDNRTIVITSHPGQIVKHGDIKCVLNEGMPIYRRPYEKGRLIIEFKVNFPENGFLSPDKLSLLEKLLPERKEVEETDEMDQVELVDFDPNQERRRHYNGEAYEDDEHHPRGGVQC |
| 预测分子量 | 52.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HSPF4重组蛋白的参考文献示例(注：HSPF4可能为虚构或非标准命名，以下内容为模拟文献，建议核实术语准确性)：
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1. **文献名称**：*Expression and Purification of Recombinant HSPF4 in E. coli for Functional Studies*
**作者**：Smith, J., et al.
**摘要**：本研究报道了HSPF4基因在大肠杆菌中的高效表达及纯化方法，通过亲和层析和尺寸排阻色谱获得高纯度蛋白，并验证其体外分子伴侣活性。
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2. **文献名称**：*HSPF4 Modulates Cellular Stress Response via Interaction with Client Proteins*
**作者**：Zhang, L., et al.
**摘要**：通过基因敲除和过表达实验，揭示了HSPF4在热应激条件下通过稳定错误折叠蛋白维持细胞稳态的机制，重组HSPF4可增强细胞抗氧化能力。
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3. **文献名称**：*Structural Characterization of Recombinant HSPF4 Using Cryo-EM*
**作者**：Johnson, R., et al.
**摘要**：利用冷冻电镜解析了HSPF4重组蛋白的三维结构，发现其独特的N端结构域在底物识别中的作用，为靶向药物设计提供依据。
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4. **文献名称**：*HSPF4 as a Potential Biomarker in Neurodegenerative Diseases: Insights from Recombinant Protein Assays*
**作者**：Wang, Y., et al.
**摘要**：通过重组HSPF4与α-突触核蛋白的体外结合实验，提出HSPF4可能通过抑制蛋白聚集延缓帕金森病进展的假说。
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**提示**：若HSPF4为笔误或非标准术语，建议参考相近蛋白(如HSPA4、HSPB4等)或进一步确认命名。实际文献需通过学术数据库(如PubMed)检索。
Heat shock protein family F member 4 (HSPF4), also known as HSPA4 or APG-2. belongs to the heat shock protein 110 (HSP110) family, a subgroup of molecular chaperones within the HSP70 superfamily. It plays a critical role in maintaining cellular proteostasis by assisting in protein folding, preventing aggregation of misfolded proteins, and facilitating stress adaptation. HSPF4 is ubiquitously expressed in eukaryotic cells and localizes primarily in the cytoplasm and nucleus. Unlike canonical HSP70s, HSPF4 exhibits ATPase activity and acts as a nucleotide exchange factor (NEF) to regulate HSP70-mediated protein refolding cycles.
Recombinant HSPF4 is produced using biotechnological platforms, such as E. coli or mammalian expression systems, to enable functional studies and therapeutic exploration. Its production involves cloning the HSPA4 gene into expression vectors, followed by purification via affinity chromatography. Recombinant HSPF4 retains chaperone activity, making it valuable for investigating protein-protein interactions, stress response pathways, and mechanisms underlying diseases like cancer, neurodegenerative disorders, and autoimmune conditions. Notably, it has been implicated in modulating immune responses by enhancing antigen presentation and regulating T-cell activation, suggesting potential applications in cancer immunotherapy. Additionally, its role in mitigating proteotoxic stress has spurred interest in developing HSPF4-based therapies for conditions linked to protein misfolding. Research continues to explore its dual roles as a cytoprotective agent and a context-dependent modulator of apoptosis, highlighting its therapeutic versatility in precision medicine.
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