| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | XRCC1 |
| Uniprot No | P18887 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-633aa |
| 氨基酸序列 | MPEIRLRHVVSCSSQDSTHCAENLLKADTYRKWRAAKAGEKTISVVLQLE KEEQIHSVDIGNDGSAFVEVLVGSSAGGAGEQDYEVLLVTSSFMSPSESR SGSNPNRVRMFGPDKLVRAAAEKRWDRVKIVCSQPYSKDSPFGLSFVRFH SPPDKDEAEAPSQKVTVTKLGQFRVKEEDESANSLRPGALFFSRINKTSP VTASDPAGPSYAAATLQASSAASSASPVSRAIGSTSKPQESPKGKRKLDL NQEEKKTPSKPPAQLSPSVPKRPKLPAPTRTPATAPVPARAQGAVTGKPR GEGTEPRRPRAGPEELGKILQGVVVVLSGFQNPFRSELRDKALELGAKYR PDWTRDSTHLICAFANTPKYSQVLGLGGRIVRKEWVLDCHRMRRRLPSQR YLMAGPGSSSEEDEASHSGGSGDEAPKLPQKQPQTKTKPTQAAGPSSPQK PPTPEETKAASPVLQEDIDIEGVQSEGQDNGAEDSGDTEDELRRVAEQKE HRLPPGQEENGEDPYAGSTDENTDSEEHQEPPDLPVPELPDFFQGKHFFL YGEFPGDERRKLIRYVTAFNGGLEDYMSDRVQFVITAQEWDPSFEEALMD NPSLAFVRPRWIYSCNEKQKLLPHQLYGVVPQA |
| 预测分子量 | 95 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于XRCC1重组蛋白的3篇代表性文献摘要：
1. **文献名称**：XRCC1 polypeptide interacts with DNA ligase III and is associated with DNA repair and recombination
**作者**：Caldecott KW et al.
**摘要**：研究通过重组XRCC1蛋白的体外实验，揭示了其与DNA连接酶III的直接结合，并证明重组蛋白可恢复XRCC1缺陷细胞的DNA断裂修复能力及对电离辐射的抗性。
2. **文献名称**：Molecular cloning of the human XRCC1 gene, which corrects defective DNA strand break repair and sister chromatid exchange
**作者**：Thompson LH et al.
**摘要**：该研究首次成功克隆XRCC1 cDNA，通过重组蛋白表达验证其功能，发现XRCC1缺失导致DNA单链断裂修复缺陷，强调了其在维持基因组稳定性中的关键作用。
3. **文献名称**：XRCC1 stimulates human DNA ligase III activity through physical interaction
**作者**：Whitehouse CJ et al.
**摘要**：利用重组XRCC1蛋白结构域分析，发现其N端结构域与DNA连接酶III结合可显著增强酶活性，揭示了XRCC1作为修复复合体支架蛋白的分子机制。
注：以上文献均发表于1990年代至2000年代初，为XRCC1功能研究的奠基性工作，涉及重组蛋白在修复机制解析中的应用。如需近年研究，可补充蛋白结构解析或疾病相关突变功能分析文献。
**Background of XRCC1 Recombinant Protein**
XRCC1 (X-ray repair cross-complementing protein 1) is a critical scaffold protein involved in DNA repair processes, particularly in the repair of single-strand breaks (SSBs) and base excision repair (BER). It functions by coordinating interactions between key repair enzymes, such as DNA ligase III, poly(ADP-ribose) polymerase (PARP), and DNA polymerase β (POLβ), ensuring efficient repair of DNA lesions caused by oxidative stress, alkylation, or ionizing radiation. XRCC1 lacks intrinsic enzymatic activity but stabilizes repair complexes, enhances enzymatic activities of partners, and facilitates substrate handover during repair.
Recombinant XRCC1 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional protein for *in vitro* studies. Its modular structure—comprising an N-terminal domain that interacts with POLβ, a central scaffolding region, and a C-terminal domain binding DNA ligase III—allows researchers to dissect repair mechanisms and protein interactions. Phosphorylation sites within XRCC1 (e.g., Ser518/Thr519), which regulate its recruitment to damage sites, are often preserved in recombinant forms to study post-translational modifications.
Applications of recombinant XRCC1 include reconstituting DNA repair pathways in cell-free systems, screening for inhibitors targeting BER/SSB repair (relevant in cancer therapy), and analyzing genetic variants linked to repair deficiencies. Mutations in XRCC1 are associated with increased genomic instability, cancer susceptibility, and neurological disorders, underscoring its biomedical relevance. The recombinant protein also aids in structural studies, drug discovery, and understanding synthetic lethality with PARP inhibitors in BRCA-deficient cancers.
Overall, XRCC1 recombinant protein serves as a vital tool for elucidating DNA repair mechanisms and developing strategies to target repair pathways in disease contexts.
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