| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | AK3 |
| Uniprot No | Q9UIJ7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-227aa |
| 氨基酸序列 | Adenylate kinase 3 alpha-like 1; Adenylate kinase 3; Adenylate kinase 3, formerly; adenylate kinase 6, adenylate kinase 3 like 1; AK 3; AK3; AK3, formerly; AK3L1; AK4; AK4, mouse, homolog of; AK6; AKL3L; AKL3L1; FIX; GTP:AMP phosphotransferase; GTP:AMP phosphotransferase, mitochondrial; KAD3_HUMAN; mitochondrial; OTTHUMP00000021015; OTTHUMP00000021016; RP11 6J24.4 |
| 预测分子量 | 32.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AK3重组蛋白的模拟参考文献示例(注：以下内容为虚构，仅用于演示格式，实际引用请查询真实数据库)：
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1. **文献名称**：*Recombinant Expression and Functional Characterization of Adenylate Kinase 3 in Escherichia coli*
**作者**：Zhang L., Wang Y., et al.
**摘要**：本研究报道了人源AK3基因在大肠杆菌系统中的重组表达与纯化，通过酶动力学分析揭示了AK3在ATP/ADP代谢中的特异性催化活性，并探讨其在线粒体能量稳态中的潜在作用。
2. **文献名称**：*Structural Insights into Mitochondrial AK3 by X-ray Crystallography*
**作者**：Smith J.R., Tanaka M., et al.
**摘要**：利用X射线晶体学解析了重组AK3蛋白的三维结构，揭示了其独特的底物结合域构象，为理解AK3与其他腺苷酸激酶亚型的差异提供了结构基础。
3. **文献名称**：*AK3 Deficiency and Compensatory Mechanisms in Cellular Energy Metabolism*
**作者**：Chen H., Kim S., et al.
**摘要**：通过构建AK3基因敲除细胞模型，结合重组AK3蛋白回补实验，证实AK3缺失导致线粒体ATP转运效率下降，并激活AMPK通路以维持能量平衡。
4. **文献名称**：*High-Yield Production of Recombinant AK3 in Mammalian Expression Systems for Drug Screening*
**作者**：Gupta R., Müller P., et al.
**摘要**：优化哺乳动物细胞表达系统实现AK3的高效分泌表达，并应用于靶向代谢疾病的小分子抑制剂筛选，验证了重组AK3在药物开发中的应用潜力。
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**提示**：实际研究中请通过PubMed、Web of Science或Google Scholar等平台检索真实文献，使用关键词如“AK3 recombinant protein”、“adenylate kinase 3 expression”等。
Adenylate kinase 3 (AK3), also known as AK3 mitochondrial, is a member of the adenylate kinase family that plays a critical role in cellular energy metabolism. Located within the mitochondrial matrix, AK3 facilitates the reversible transfer of phosphate groups between nucleotides, primarily catalyzing the conversion of GTP and AMP to GDP and ADP. This reaction contributes to maintaining nucleotide balance and energy homeostasis, particularly in tissues with high energy demands such as the liver, heart, and skeletal muscle. Unlike other adenylate kinase isoforms, AK3’s substrate specificity for GTP instead of ATP reflects its unique adaptation to mitochondrial metabolic pathways.
The recombinant AK3 protein is produced using genetic engineering techniques, typically through expression in bacterial or eukaryotic systems. Its recombinant form allows researchers to study the enzyme’s structure, kinetics, and interactions in vitro, bypassing challenges associated with isolating it from native tissues. Structural studies have revealed conserved motifs critical for nucleotide binding and catalytic activity, offering insights into its regulatory mechanisms. Dysregulation of AK3 has been implicated in mitochondrial disorders, neurodegenerative diseases, and cancer, highlighting its potential as a therapeutic target or biomarker.
Research on recombinant AK3 has advanced understanding of mitochondrial bioenergetics, nucleotide metabolism, and cellular stress responses. It also serves as a tool for drug screening and mechanistic studies in pathologies linked to energy dysregulation. Despite progress, questions remain about its tissue-specific roles and post-translational modifications. Ongoing studies aim to unravel its broader biological significance beyond energy metabolism, including potential involvement in apoptosis and redox signaling.
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