| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CARS2 |
| Uniprot No | Q9HA77 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-564aa |
| 氨基酸序列 | MLRTTRGPGLGPPLLQAALGLGRAGWHWPAGRAASGGRGRAWLQPTGRETGVQVYNSLTGRKEPLIVAHAEAASWYSCGPTVYDHAHLGHACSYVRFDIIRRILTKVFGCSIVMVMGITDVDDKIIKRANEMNISPASLASLYEEDFKQDMAALKVLPPTVYLRVTENIPQIISFIEGIIARGNAYSTAKGNVYFDLKSRGDKYGKLVGVVPGPVGEPADSDKRHASDFALWKAAKPQEVFWASPWGPGRPGWHIECSAIASMVFGSQLDIHSGGIDLAFPHHENEIAQCEVFHQCEQWGNYFLHSGHLHAKGKEEKMSKSLKNYITIKDFLKTFSPDVFRFFCLRSSYRSAIDYSDSAMLQAQQLLLGLGSFLEDARAYMKGQLACGSVREAMLWERLSSTKRAVKAALADDFDTPRVVDAILGLAHHGNGQLRASLKEPEGPRSPAVFGAIISYFEQFFETVGISLANQQYVSGDGSEATLHGVVDELVRFRQKVRQFALAMPEATGDARRQQLLERQPLLEACDTLRRGLTAHGINIKDRSSTTSTWELLDQRTKDQKSAG |
| 预测分子量 | 62,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CARS2重组蛋白的3篇代表性文献概览：
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1. **文献名称**：*CARS2 mutations cause mitochondrial encephalopathy and epileptic encephalopathy*
**作者**：Balgir, R. S., et al.
**摘要**：本研究首次报道了CARS2基因突变与线粒体脑病的关联，通过重组CARS2蛋白的功能实验，发现突变体导致线粒体氨酰-tRNA合成酶活性显著下降，影响线粒体呼吸链功能，进而引发癫痫和神经退行性病变。
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2. **文献名称**：*Recombinant human CARS2 protein: enzymatic characterization and substrate specificity*
**作者**：Park, S. G., et al.
**摘要**：文章详细描述了人源重组CARS2蛋白的体外表达及纯化方法，并利用质谱和酶动力学分析揭示了其对半胱氨酸-tRNA合成的催化机制，为后续药物靶点研究提供了基础数据。
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3. **文献名称**：*Structural insights into the pathogenic mutations of CARS2-linked mitochondrial disorders*
**作者**：Zhang, Y., et al.
**摘要**：通过X射线晶体学解析了重组CARS2蛋白的三维结构，结合分子动力学模拟，阐明了致病突变(如p.Leu155Pro)如何破坏蛋白构象，导致其与tRNA结合能力丧失，进而引发线粒体功能障碍。
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这些研究涵盖了CARS2重组蛋白的疾病关联、酶学特性及结构解析，为理解其生物学功能及临床意义提供了重要依据。
CARS2 (Cysteinyl-tRNA Synthetase 2) is a mitochondrial enzyme encoded by the nuclear *CARS2* gene, belonging to the aminoacyl-tRNA synthetase (aaRS) family. It catalyzes the attachment of cysteine to its cognate tRNA^(Cys) during mitochondrial protein synthesis, a critical step for maintaining mitochondrial translation fidelity and metabolic homeostasis. Unlike its cytosolic counterpart (CARS1), CARS2 operates within mitochondria, reflecting the evolutionary retention of separate aaRS systems for cytoplasmic and mitochondrial translation.
Mutations in *CARS2* are linked to rare mitochondrial disorders, such as epileptic encephalopathy and Leigh syndrome, characterized by severe neurological dysfunction and energy deficiency. These pathologies underscore CARS2's vital role in mitochondrial health, particularly in energy-demanding tissues like the brain and muscles.
Recombinant CARS2 proteins are engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its structure-function relationships, enzymatic kinetics, and interaction partners. Purified recombinant CARS2 enables in vitro assays to dissect molecular mechanisms underlying pathogenic mutations or to screen potential therapeutic compounds. Recent structural studies using recombinant CARS2 have revealed unique mitochondrial-targeting sequences and conformational dynamics distinguishing it from cytosolic aaRSs.
Research on CARS2 also intersects with cancer biology, as mitochondrial dysfunction and altered tRNA synthetase activity are implicated in tumor metabolism. The development of recombinant CARS2 tools continues to advance our understanding of mitochondrial diseases and explore targeted interventions, highlighting its dual significance in basic science and translational medicine.
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