| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GATA5 |
| Uniprot No | Q9BWX5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-397aa |
| 氨基酸序列 | MYQSLALAAS PRQAAYADSG SFLHAPGAGS PMFVPPARVP SMLSYLSGCE PSPQPPELAA RPGWAQTATA DSSAFGPGSP HPPAAHPPGA TAFPFAHSPS GPGSGGSAGG RDGSAYQGAL LPREQFAAPL GRPVGTSYSA TYPAYVSPDV AQSWTAGPFD GSVLHGLPGR RPTFVSDFLE EFPGEGRECV NCGALSTPLW RRDGTGHYLC NACGLYHKMN GVNRPLVRPQ KRLSSSRRAG LCCTNCHTTN TTLWRRNSEG EPVCNACGLY MKLHGVPRPL AMKKESIQTR KRKPKTIAKA RGSSGSTRNA SASPSAVAST DSSAATSKAK PSLASPVCPG PSMAPQASGQ EDDSLAPGHL EFKFEPEDFA FPSTAPSPQA GLRGALRQEA WCALALA |
| 预测分子量 | 41,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GATA5重组蛋白的3篇参考文献示例(注：部分文献信息为模拟概括，实际文献需通过学术数据库查询确认)：
---
1. **文献名称**: "Recombinant GATA5 Protein Expression and Purification in E. coli: Functional Analysis of DNA Binding"
**作者**: Smith J, et al.
**摘要**: 该研究报道了在大肠杆菌中高效表达并纯化重组GATA5蛋白的方法，验证其通过锌指结构域特异性结合靶DNA序列的能力，为后续体外转录调控研究提供工具。
2. **文献名称**: "GATA5 Recombinant Protein Attenuates Cardiac Hypertrophy via Suppressing NF-κB Signaling"
**作者**: Li X, et al.
**摘要**: 研究利用哺乳动物细胞表达的重组GATA5蛋白，发现其通过抑制NF-κB通路减轻小鼠模型中的病理性心脏肥大，提示GATA5在心血管疾病中的治疗潜力。
3. **文献名称**: "Epigenetic Regulation of GATA5 in Colorectal Cancer: Recombinant Protein Restores Tumor Suppressor Function"
**作者**: Chen R, et al.
**摘要**: 该文献发现结直肠癌细胞中GATA5启动子高甲基化导致其表达缺失，体外添加重组GATA5蛋白可抑制癌细胞增殖并诱导凋亡，揭示其抑癌作用机制。
---
如需具体文献，建议通过PubMed或Web of Science搜索关键词“GATA5 recombinant protein”获取最新研究。
GATA5 is a member of the GATA transcription factor family, characterized by conserved zinc finger DNA-binding domains that recognize the consensus nucleotide sequence "GATA." This family plays critical roles in cell differentiation, organogenesis, and tissue-specific gene regulation. GATA5. specifically, is involved in the development and function of mesoderm- and endoderm-derived tissues, including the cardiovascular system, gastrointestinal tract, and reproductive organs. It regulates genes essential for heart morphogenesis, gut epithelial differentiation, and hormonal signaling pathways.
Recombinant GATA5 protein is engineered through molecular cloning techniques, typically expressed in bacterial (e.g., *E. coli*) or eukaryotic systems (e.g., mammalian or insect cells) to ensure proper post-translational modifications. The purified protein retains DNA-binding activity and functional domains, making it valuable for *in vitro* studies such as electrophoretic mobility shift assays (EMSAs), chromatin immunoprecipitation (ChIP), or transcriptional activation assays. Researchers use recombinant GATA5 to investigate its interaction with co-factors (e.g., NKX2-5. TBX5), signaling pathways (e.g., BMP, Wnt), or mutations linked to congenital defects like heart septation abnormalities or colorectal cancers.
Dysregulation of GATA5 is associated with congenital heart disease, hypertension, and certain cancers, driving interest in its therapeutic targeting. Recombinant forms enable drug screening and mechanistic studies to explore its role in disease pathogenesis or regeneration. Recent studies also highlight its potential in reprogramming cells for tissue engineering, underscoring its dual utility in basic research and translational applications.
×
