| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COPb1 |
| Uniprot No | P53618 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-953aa |
| 氨基酸序列 | MTAAENVCYTLINVPMDSEPPSEISLKNDLEKGDVKSKTEALKKVIIMILNGEKLPGLLMTIIRFVLPLQDHTIKKLLLVFWEIVPKTTPDGRLLHEMILVCDAYRKDLQHPNEFIRGSTLRFLCKLKEAELLEPLMPAIRACLEHRHSYVRRNAVLAIYTIYRNFEHLIPDAPELIHDFLVNEKDASCKRNAFMMLIHADQDRALDYLSTCIDQVQTFGDILQLVIVELIYKVCHANPSERARFIRCIYNLLQSSSPAVKYEAAGTLVTLSSAPTAIKAAAQCYIDLIIKESDNNVKLIVLDRLIELKEHPAHERVLQDLVMDILRVLSTPDLEVRKKTLQLALDLVSSRNVEELVIVLKKEVIKTNNVSEHEDTDKYRQLLVRTLHSCSVRFPDMAANVIPVLMEFLSDNNEAAAADVLEFVREAIQRFDNLRMLIVEKMLEVFHAIKSVKIYRGALWILGEYCSTKEDIQSVMTEIRRSLGEIPIVESEIKKEAGELKPEEEITVGPVQKLVTEMGTYATQSALSSSRPTKKEEDRPPLRGFLLDGDFFVAASLATTLTKIALRYVALVQEKKKQNSFVAEAMLLMATILHLGKSSLPKKPITDDDVDRISLCLKVLSECSPLMNDIFNKECRQSLSHMLSAKLEEEKLSQKKESEKRNVTVQPDDPISFMQLTAKNEMNCKEDQFQLSLLAAMGNTQRKEAADPLASKLNKVTQLTGFSDPVYAEAYVHVNQYDIVLDVLVVNQTSDTLQNCTLELATLGDLKLVEKPSPLTLAPHDFANIKANVKVASTENGIIFGNIVYDVSGAASDRNCVVLSDIHIDIMDYIQPATCTDAEFRQMWAEFEWENKVTVNTNMVDLNDYLQHILKSTNMKCLTPEKALSGYCGFMAANLYARSIFGEDALANVSIEKPIHQGPDAAVTGHIRIRAKSQGMALSLGDKINLSQKKTSI |
| 预测分子量 | 107 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **COPB1重组蛋白** 的虚构参考文献示例(仅供参考，实际文献需通过学术平台检索)：
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1. **文献名称**: *"Recombinant COPB1 Protein Facilitates In Vitro Vesicle Formation in COPII-Mediated Transport"*
**作者**: Smith A, et al.
**摘要**: 研究报道了通过大肠杆菌表达系统纯化COPB1重组蛋白，并验证其在COPII复合体中的功能。实验表明，COPB1对内质网到高尔基体的囊泡形成具有关键作用，重组蛋白可恢复囊泡运输缺陷的细胞模型表型。
2. **文献名称**: *"Structural Insights into COPB1 in Membrane Protein Sorting by Cryo-EM"*
**作者**: Zhang Y, et al.
**摘要**: 通过冷冻电镜解析COPB1重组蛋白与COPII复合体的三维结构，揭示了其与Sec23/Sec24亚基的相互作用机制，为理解膜蛋白分选提供了结构基础。
3. **文献名称**: *"COPB1 Mutations Disrupt Cellular Trafficking: Rescue by Recombinant Protein Expression"*
**作者**: Lee H, et al.
**摘要**: 研究分析了COPB1基因突变导致的内质网应激表型，并通过外源性重组COPB1蛋白导入细胞，证明了其在恢复蛋白质分泌通路中的治疗潜力。
4. **文献名称**: *"Functional Characterization of COPB1 in Viral Replication Using Recombinant Assays"*
**作者**: Wang X, et al.
**摘要**: 利用COPB1重组蛋白进行体外结合实验，发现其与特定病毒蛋白相互作用，可能通过干扰宿主细胞运输通路促进病毒颗粒组装。
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如需真实文献，建议通过 **PubMed** 或 **Google Scholar** 检索关键词“COPB1 recombinant protein”或“COPB1 function”。
COPb1 (Coatomer Protein Complex Subunit Beta 1) is a critical component of the COPI (Coat Protein Complex I) complex, which plays a central role in intracellular vesicular trafficking. The COPI complex is primarily involved in retrograde transport of proteins and lipids from the Golgi apparatus to the endoplasmic reticulum (ER), ensuring proper sorting, recycling, and maintenance of organelle integrity. As one of seven subunits (α, β, β', γ, δ, ε, ζ) forming the COPI complex, COPb1 (β-subunit) contributes to the structural stability of the coatomer and facilitates interactions with other trafficking machinery components, such as Arf1 (ADP-ribosylation factor 1), which regulates COPI vesicle formation.
Recombinant COPb1 protein is engineered to study COPI-mediated trafficking mechanisms, protein-protein interactions, and regulatory pathways in eukaryotic cells. Its production typically involves heterologous expression systems (e.g., *E. coli* or mammalian cells) to obtain purified, functional protein for biochemical assays, structural studies, or drug screening. Researchers utilize recombinant COPb1 to investigate diseases linked to COPI dysfunction, including cancers, neurodegenerative disorders (e.g., Alzheimer’s), and genetic conditions affecting secretory pathways. For instance, COPI dysregulation has been implicated in disrupted ER-Golgi homeostasis, leading to misfolded protein accumulation or aberrant signaling.
Additionally, recombinant COPb1 serves as a tool to explore post-translational modifications (e.g., phosphorylation) that modulate COPI assembly/disassembly. Its study enhances understanding of cellular logistics, therapeutic targets, and evolutionary conservation of vesicular transport systems.
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