| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ODF1 |
| Uniprot No | Q14990 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-250aa |
| 氨基酸序列 | MAALSCLLDS VRRDIKKVDR ELRQLRCIDE FSTRCLCDLY MHPYCCCDLH PYPYCLCYSK RSRSCGLCDL YPCCLCDYKL YCLRPSLRSL ERKAIRAIED EKRELAKLRR TTNRILASSC CSSNILGSVN VCGFEPDQVK VRVKDGKVCV SAERENRYDC LGSKKYSYMN ICKEFSLPPC VDEKDVTYSY GLGSCVKIES PCYPCTSPCS PCSPCSPCNP CSPCNPCSPY DPCNPCYPCG SRFSCRKMIL |
| 预测分子量 | 28,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于ODF1(Outer Dense Fiber Protein 1)重组蛋白研究的模拟参考文献示例(实际文献需通过学术数据库查询)：
1. **文献名称**: *Molecular Cloning and Characterization of ODF1 in Mammalian Sperm*
**作者**: Smith A, et al.
**摘要**: 报道了ODF1基因的克隆及其重组蛋白的表达，发现其在哺乳动物精子尾部结构形成中起关键作用，可能与精子运动功能相关。
2. **文献名称**: *ODF1 Interaction with Sperm-specific Proteins during Spermiogenesis*
**作者**: Tanaka K, et al.
**摘要**: 通过重组蛋白实验，揭示了ODF1与SPAG5等精子特异性蛋白的互作机制，提出其在精子形成过程中参与细胞骨架组装。
3. **文献名称**: *Recombinant ODF1 as a Potential Biomarker in Male Infertility*
**作者**: Chen L, et al.
**摘要**: 利用重组ODF1蛋白开发检测方法，发现男性不育患者精液中ODF1表达异常，提示其可作为诊断标志物。
4. **文献名称**: *Structural Analysis of ODF1 Recombinant Protein via Cryo-EM*
**作者**: Müller R, et al.
**摘要**: 采用冷冻电镜解析重组ODF1蛋白的三维结构，阐明其纤维状组装模式及可能的二硫键网络。
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注：以上为模拟示例，实际文献需通过PubMed、Google Scholar等平台检索。建议使用关键词 "ODF1 recombinant protein" 或 "ODF1 gene expression" 查找最新研究。
**Background of ODF1 Recombinant Protein**
ODF1 (Outer Dense Fiber Protein 1) is a structural component critical for sperm tail development and function. It is a major protein of the outer dense fibers (ODFs), cytoskeletal structures that reinforce the sperm flagellum, ensuring mechanical stability and motility. ODFs are unique to spermatozoa and play a vital role in maintaining the structural integrity of the flagellum during the intense mechanical stresses of sperm propulsion.
The ODF1 gene encodes a protein rich in cysteine and proline residues, contributing to its high stability through disulfide bonds. Studies suggest ODF1 interacts with other sperm components, such as microtubules and mitochondrial sheath proteins, to coordinate flagellar movement. Mutations or dysregulation of ODF1 have been linked to male infertility, highlighting its essential role in spermatogenesis and sperm function.
Recombinant ODF1 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cell lines) to enable functional studies. This engineered protein retains key structural domains, allowing researchers to investigate its biochemical properties, protein-protein interactions, and role in sperm development. Recombinant ODF1 is particularly valuable for studying infertility mechanisms, developing diagnostic markers, or exploring contraceptive strategies targeting sperm motility.
Recent research also explores ODF1's potential involvement in cellular processes beyond spermatogenesis, such as cancer progression, though its primary significance remains in reproductive biology. The availability of recombinant ODF1 facilitates antibody production, structural analysis, and *in vitro* assays, advancing our understanding of sperm biology and related pathologies.
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