| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZP1 |
| Uniprot No | P60852 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 26-601aa |
| 氨基酸序列 | RWLQPDPGLPGLRHSYDCGIKGMQLLVFPRPGQTLRFKVVDEFGNRFDVNNCSICYHWVT SRPQEPAVFSADYRGCHVLEKDGRFHLRVFMEAVLPNGRVDVAQDATLICPKPDPSRTLD SQLAPPAMFSVSTPQTLSFLPTSGHTSQGSGHAFPSPLDPGHSSVHPTPALPSPGPGPTL ATLAQPHWGTLEHWDVNKRDYIGTHLSQEQCQVASGHLPCIVRRTSKEACQQAGCCYDNT REVPCYYGNTATVQCFRDGYFVLVVSQEMALTHRITLANIHLAYAPTSCSPTQHTEAFVV FYFPLTHCGTTMQVAGDQLIYENWLVSGIHIQKGPQGSITRDSTFQLHVRCVFNASDFLP IQASIFPPPSPAPMTQPGPLRLELRIAKDETFSSYYGEDDYPIVRLLREPVHVEVRLLQR TDPNLVLLLHQCWGAPSANPFQQPQWPILSDGCPFKGDSYRTQMVALDGATPFQSHYQRF TVATFALLDSGSQRALRGLVYLFCSTSACHTSGLETCSTACSTGTTRQRRSSGHRNDTAR PQDIVSSPGPVGFEDSYGQEPTLGPTDSNGNSSLRP |
| 预测分子量 | 70 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ZP1重组蛋白的参考文献示例(注：以下内容为模拟生成，非真实文献)：
1. **文献名称**：*Recombinant Human ZP1 Protein Expression and Characterization in Mammalian Cells*
**作者**：Liang, X., Zhang, Y., et al.
**摘要**：该研究通过哺乳动物细胞表达系统成功表达并纯化了重组人ZP1蛋白，验证了其糖基化修饰及与精子结合的关键结构域，为研究ZP1在受精中的作用提供了工具。
2. **文献名称**：*Immunocontraceptive Potential of Recombinant ZP1 in Mouse Models*
**作者**：Gupta, S.K., Govind, C.K.
**摘要**：研究利用重组ZP1蛋白作为抗原免疫雌性小鼠，发现其能诱导高滴度抗体，显著降低生育率，但部分个体出现卵巢功能异常，提示需优化免疫策略。
3. **文献名称**：*Structural Insights into ZP1 Protein via Cryo-EM Analysis*
**作者**：Hinsdale, T., Boja, E.S.
**摘要**：通过冷冻电镜技术解析了重组ZP1蛋白的三维结构，揭示了其多聚体组装模式及与ZP2/ZP3的相互作用机制，为透明带形成研究提供新视角。
4. **文献名称**：*Comparative Analysis of ZP1 Expression Systems: E. coli vs. Yeast*
**作者**：Chakravarty, S., et al.
**摘要**：对比了大肠杆菌和酵母系统表达的重组ZP1蛋白，发现酵母系统能实现更接近天然结构的糖基化，但大肠杆菌表达产量更高，讨论了不同应用场景下的选择策略。
(提示：如需真实文献，建议通过PubMed、Google Scholar等平台检索关键词“recombinant ZP1 protein”或“ZP1 zona pellucida”。)
**Background of ZP1 Recombinant Protein**
Zona pellucida protein 1 (ZP1) is a critical glycoprotein component of the zona pellucida (ZP), an extracellular matrix surrounding mammalian oocytes. The ZP plays essential roles in oocyte development, fertilization, and early embryo protection. Among the four primary ZP glycoproteins (ZP1–4 in humans), ZP1 contributes to the structural integrity of the ZP matrix by cross-linking filaments formed by ZP2 and ZP3. Its unique cysteine-rich domain facilitates disulfide bond formation, stabilizing the 3D network necessary for sperm binding and species-specific fertilization.
Recombinant ZP1 protein is produced using biotechnological platforms (e.g., mammalian cell lines, *E. coli*, or yeast) to enable detailed functional and structural studies. Unlike native ZP1. which undergoes complex post-translational modifications (e.g., glycosylation) in vivo, recombinant variants allow researchers to dissect domain-specific functions, antigenicity, and interactions with sperm surface receptors. This has been pivotal in understanding infertility mechanisms linked to ZP1 mutations, which disrupt ZP assembly and are associated with conditions like premature ovarian insufficiency.
In reproductive biology, ZP1 recombinant protein serves as a tool for developing contraceptive vaccines, as antibodies targeting ZP1 can block sperm-egg interaction. However, challenges remain in mimicking native glycosylation patterns, which influence immunogenicity and efficacy. Additionally, recombinant ZP1 aids in designing diagnostic assays for anti-zona antibodies, a factor in some infertility cases.
Recent studies also explore ZP1's role in biomaterial engineering, leveraging its self-assembling properties for drug delivery or tissue scaffolding. Despite progress, ethical and technical hurdles persist, particularly in balancing immunocontraceptive applications with preserving ovarian function. Overall, ZP1 recombinant protein remains a vital resource for advancing reproductive medicine and bioengineering.
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