| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | LPD |
| Uniprot No | P90597 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-477aa |
| 氨基酸序列 | MFRRCAVKLN PYDVVVIGGG PGGYVASIKA AQLGMKTACV EKRGALGGTC LNVGCIPSKA LLHATHVYHD AHANFARYGL MGGEGVTMDS AKMQQQKERA VKGLTGGVEY LFKKNKVTYY KGEGSFETAH SIRVNGLDGK QEMFETKKTI IATGSEPTEL PFLPFDEKVV LSSTGALALP RVPKTMVVIG GGVIGLELGS VWARLGAKVT VVEFAPRCAP TLDEDVTNAL VGALAKNEKM KFMTSTKVVG GTNNGDSVSL EVEGKNGKRE TVTCEALLVS VGRRPFTGGL GLDKINVAKN ERGFVKIGDH FETSIPDVYA IGDVVDKGPM LAHKAEDEGV ACAEILAGKP GHVNYGVIPA VIYTMPEVAS VGKSEEELKK EGVAYKVGKF PFNANSRAKA VSTEDGFVKV LVDKATDRIL GVHIVCTTAG ELIGEACLAM EYGASSEDVG RTCHAHPTMS EALKEACMAL VAKTINF |
| 预测分子量 | 50,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LPD重组蛋白的模拟参考文献示例(内容为虚构，供格式参考)：
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1. **文献名称**：*Structural and Functional Analysis of LPD Recombinant Protein in Bacterial Expression Systems*
**作者**：Zhang, Y. et al.
**摘要**：研究利用大肠杆菌表达系统高效表达LPD重组蛋白，通过X射线晶体学解析其三维结构，并验证其在氧化还原反应中的催化活性，为酶工程应用提供基础。
2. **文献名称**：*LPD Nanoparticles as Vaccine Carriers: Enhanced Immune Response via Recombinant Antigen Delivery*
**作者**：Smith, J.R. & Patel, K.
**摘要**：开发基于LPD(脂质-多聚体-DNA)纳米颗粒的重组蛋白递送系统，证明其可显著增强模型抗原的细胞免疫应答，为新型疫苗设计提供策略。
3. **文献名称**：*Role of Recombinant Lpd Protein in Mitochondrial Metabolism Disorders*
**作者**：Lee, H. et al.
**摘要**：探讨重组Lpd(二氢硫辛酰胺脱氢酶)蛋白对线粒体功能障碍的调控作用，发现其过表达可改善代谢异常，提示潜在治疗代谢疾病的价值。
4. **文献名称**：*Optimization of LPD Recombinant Protein Production in Yeast for Industrial Applications*
**作者**：Gomez, M. et al.
**摘要**：通过酵母表达系统优化LPD重组蛋白的规模化生产，分析不同发酵条件对蛋白产量及稳定性的影响，推动其在生物制造中的应用。
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**备注**：以上文献信息为示例，实际研究中请通过PubMed、Web of Science等平台检索真实文献，关键词可尝试“LPD recombinant protein”、“Lipoprotein nanoparticles”、“Lpd enzyme expression”等。
LPD (L-alanine dehydrogenase) recombinant protein is a biotechnologically engineered antigen derived from *Mycobacterium tuberculosis* (Mtb), the causative agent of tuberculosis (TB). This enzyme plays a critical role in bacterial metabolism, catalyzing the reversible conversion of L-alanine to pyruvate and ammonia, which is essential for energy production and nitrogen assimilation. In Mtb, LPD is also part of the multifunctional antigen complex Ag85B-ESAT-6-Lpd, contributing to its pathogenicity and interaction with host immune systems.
Due to its strong immunogenicity, LPD recombinant protein has been extensively studied for TB diagnostics and vaccine development. It elicits robust T-cell responses in infected individuals, making it a valuable component in interferon-gamma release assays (IGRAs) like the QuantiFERON-TB Gold test, which differentiates latent TB infection from active disease or BCG vaccination. Additionally, LPD’s inclusion in subunit vaccine candidates aims to enhance protective immunity by targeting multiple stages of Mtb’s life cycle.
Produced via recombinant DNA technology in *E. coli* expression systems, the protein is purified using affinity chromatography, often with a His-tag for efficient isolation. Its stability and specificity have also spurred interest in structural studies to optimize antigen design and understand drug resistance mechanisms. Beyond TB, LPD’s conserved enzymatic role in other pathogens highlights its broader potential in microbial research and therapeutic innovation. Ongoing studies focus on improving its diagnostic accuracy and vaccine efficacy, addressing global TB control challenges.
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