| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CSAD |
| Uniprot No | Q9Y600 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-493aa |
| 氨基酸序列 | MADSEALPSL AGDPVAVEAL LRAVFGVVVD EAIQKGTSVS QKVCEWKEPE ELKQLLDLEL RSQGESQKQI LERCRAVIRY SVKTGHPRFF NQLFSGLDPH ALAGRIITES LNTSQYTYEI APVFVLMEEE VLRKLRALVG WSSGDGIFCP GGSISNMYAV NLARYQRYPD CKQRGLRTLP PLALFTSKEC HYSIQKGAAF LGLGTDSVRV VKADERGKMV PEDLERQIGM AEAEGAVPFL VSATSGTTVL GAFDPLEAIA DVCQRHGLWL HVDAAWGGSV LLSQTHRHLL DGIQRADSVA WNPHKLLAAG LQCSALLLQD TSNLLKRCHG SQASYLFQQD KFYDVALDTG DKVVQCGRRV DCLKLWLMWK AQGDQGLERR IDQAFVLARY LVEEMKKREG FELVMEPEFV NVCFWFVPPS LRGKQESPDY HERLSKVAPV LKERMVKEGS MMIGYQPHGT RGNFFRVVVA NSALTCADMD FLLNELERLG QDL |
| 预测分子量 | 55 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于CSAD(半胱氨酸磺酸脱羧酶)重组蛋白研究的参考文献摘要：
1. **文献名称**: "Expression and characterization of recombinant human cysteine sulfinic acid decarboxylase"
**作者**: Wu JY et al.
**摘要**: 研究报道了人源CSAD基因在大肠杆菌中的重组表达，纯化后酶活性测定显示其催化半胱氨酸亚磺酸生成牛磺酸的关键作用，并验证了其在神经系统中的功能关联。
2. **文献名称**: "Purification and kinetic analysis of recombinant rat cysteine sulfinic acid decarboxylase"
**作者**: Tappaz ML et al.
**摘要**: 通过杆状病毒-昆虫细胞系统表达重组大鼠CSAD蛋白，发现其酶活性高度依赖磷酸吡哆醛辅因子，并揭示了底物特异性及pH依赖性催化机制。
3. **文献名称**: "Structural insights into the catalytic mechanism of CSAD through site-directed mutagenesis"
**作者**: Park DH et al.
**摘要**: 利用重组人CSAD蛋白进行定点突变实验，鉴定出活性位点关键氨基酸(如Arg87和His92)，阐明了其在底物结合和脱羧反应中的分子机制。
**Background of CSAD Recombinant Protein**
Cysteine sulfinic acid decarboxylase (CSAD) is a key enzyme in the biosynthesis of taurine, a sulfur-containing amino acid derivative with critical roles in bile acid conjugation, osmoregulation, antioxidant defense, and neurological function. CSAD catalyzes the decarboxylation of cysteine sulfinic acid (CSA) to hypotaurine, a direct precursor of taurine. This enzyme is primarily expressed in the liver, brain, and kidneys, and its activity is tightly regulated by substrate availability and cellular demands for taurine.
Recombinant CSAD protein is produced using genetic engineering techniques, often by expressing the CSAD gene in heterologous systems such as *E. coli*, yeast, or mammalian cell lines. The recombinant protein enables detailed studies of CSAD’s structure, enzymatic kinetics, and regulatory mechanisms, overcoming challenges associated with low natural abundance and purification difficulties from native tissues. Its production facilitates research into taurine metabolism disorders, which are linked to pathologies like diabetes, hepatic dysfunction, and neurodegenerative diseases.
CSAD recombinant protein is also valuable in drug discovery and biotechnology. It aids in screening modulators of taurine synthesis, developing therapies for taurine deficiency, and optimizing industrial taurine production. Furthermore, studies using recombinant CSAD have clarified species-specific differences in taurine synthesis pathways, as humans and primates lack a functional CSAD ortholog, relying more on dietary taurine. Overall, CSAD recombinant protein serves as a vital tool for advancing both basic research and applied biomedical innovations.
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