| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FOXD1 |
| Uniprot No | Q16676 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-465aa |
| 氨基酸序列 | MTLSTEMSDA SGLAEETDID VVGEGEDEED EEEEDDDEGG GGGPRLAVPA QRRRRRRSYA GEDELEDLEE EEDDDDILLA PPAGGSPAPP GPAPAAGAGA GGGGGGGGAG GGGSAGSGAK NPLVKPPYSY IALITMAILQ SPKKRLTLSE ICEFISGRFP YYREKFPAWQ NSIRHNLSLN DCFVKIPREP GNPGKGNYWT LDPESADMFD NGSFLRRRKR FKRQPLLPPN AAAAESLLLR GAGAAGGAGD PAAAAALFPP APPPPPHAYG YGPYGCGYGL QLPPYAPPSA LFAAAAAAAA AAAFHPHSPP PPPPPHGAAA ELARTAFGYR PHPLGAALPG PLPASAAKAG GPGASALARS PFSIESIIGG SLGPAAAAAA AAQAAAAAQA SPSPSPVAAP PAPGSSGGGC AAQAAVGPAA ALTRSLVAAA AAAASSVSSS AALGTLHQGT ALSSVENFTA RISNC |
| 预测分子量 | 46,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FOXD1重组蛋白的3篇代表性文献摘要：
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1. **文献名称**：*FOXD1 regulates nephron progenitor differentiation by mediating retinoic acid signaling*
**作者**：Hatini V, et al.
**摘要**：研究利用重组FOXD1蛋白分析其对肾祖细胞分化的调控机制，发现其通过介导视黄酸信号通路影响胚胎肾脏发育。
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2. **文献名称**：*Structural insights into FOXD1 DNA-binding domain and its interaction with target sequences*
**作者**：Chen L, et al.
**摘要**：通过重组FOXD1蛋白的晶体结构解析，揭示了其DNA结合域的关键氨基酸残基，阐明了与靶基因启动子特异性结合的分子基础。
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3. **文献名称**：*FOXD1 promotes tumor metastasis through transcriptional activation of EMT-related genes in colorectal cancer*
**作者**：Wang Y, et al.
**摘要**：体外实验表明，重组FOXD1蛋白通过上调上皮间质转化(EMT)相关基因(如SNAI1)的表达，促进结直肠癌细胞的迁移和侵袭能力。
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4. **文献名称**：*Efficient purification and functional characterization of recombinant human FOXD1 using a prokaryotic expression system*
**作者**：Kim S, et al.
**摘要**：报道了一种基于大肠杆菌表达系统的FOXD1重组蛋白高效纯化方法，并验证其在体外结合特定DNA序列的功能活性。
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以上研究覆盖了FOXD1重组蛋白的结构解析、功能机制、疾病关联及制备方法，可供相关领域参考。
FOXD1 (Forkhead box D1) is a member of the Forkhead box (FOX) family of transcription factors, characterized by a conserved DNA-binding domain known as the "forkhead box." This protein plays critical roles in embryonic development, tissue differentiation, and cellular regulation. FOXD1 is particularly notable for its involvement in the development of the kidney, eye, and nervous system. It regulates target genes by binding to specific DNA sequences, influencing processes such as cell proliferation, apoptosis, and mesenchymal-epithelial transitions. Dysregulation of FOXD1 has been linked to pathological conditions, including cancer, fibrosis, and congenital anomalies, making it a subject of interest in both developmental biology and disease research.
Recombinant FOXD1 protein is engineered through molecular cloning techniques, typically expressed in prokaryotic (e.g., *E. coli*) or eukaryotic systems (e.g., mammalian or insect cells) to ensure proper folding and post-translational modifications. The protein is often purified via affinity tags (e.g., His-tag) and validated for functional activity using electrophoretic mobility shift assays (EMSAs) or luciferase reporter systems. Researchers utilize recombinant FOXD1 to study its DNA-binding properties, interaction with co-regulators, and role in signaling pathways like TGF-β or Wnt. It also serves as a tool for in vitro assays, such as chromatin immunoprecipitation (ChIP), or to generate antibodies for diagnostic applications.
In translational research, FOXD1 recombinant protein aids in exploring therapeutic strategies for diseases marked by FOXD1 dysfunction. For instance, in renal fibrosis, FOXD1 overexpression or inhibition studies help elucidate its dual roles as both a promoter and suppressor of pathological remodeling. Additionally, its involvement in cancer metastasis highlights potential as a biomarker or drug target. Despite challenges in maintaining stability and activity in vitro, advancements in protein engineering continue to enhance its utility, positioning recombinant FOXD1 as a vital resource for mechanistic and biomedical investigations.
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