| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TINAG |
| Uniprot No | Q9UJW2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-476aa |
| 氨基酸序列 | MWTGYKILIF SYLTTEIWME KQYLSQREVD LEAYFTRNHT VLQGTRFKRA IFQGQYCRNF GCCEDRDDGC VTEFYAANAL CYCDKFCDRE NSDCCPDYKS FCREEKEWPP HTQPWYPEGC FKDGQHYEEG SVIKENCNSC TCSGQQWKCS QHVCLVRSEL IEQVNKGDYG WTAQNYSQFW GMTLEDGFKF RLGTLPPSPM LLSMNEMTAS LPATTDLPEF FVASYKWPGW THGPLDQKNC AASWAFSTAS VAADRIAIQS KGRYTANLSP QNLISCCAKN RHGCNSGSID RAWWYLRKRG LVSHACYPLF KDQNATNNGC AMASRSDGRG KRHATKPCPN NVEKSNRIYQ CSPPYRVSSN ETEIMKEIMQ NGPVQAIMQV REDFFHYKTG IYRHVTSTNK ESEKYRKLQT HAVKLTGWGT LRGAQGQKEK FWIAANSWGK SWGENGYFRI LRGVNESDIE KLIIAAWGQL TSSDEP |
| 预测分子量 | 54,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TINAG(Tubulointerstitial Nephritis Antigen)重组蛋白的假设性参考文献示例(非真实文献，仅作格式和内容参考)：
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1. **文献名称**: *Expression and Purification of Recombinant TINAG in E. coli for Autoimmune Nephritis Studies*
**作者**: Smith A, et al.
**摘要**: 本研究报道了在大肠杆菌中高效表达可溶性TINAG重组蛋白的优化方法，通过His标签亲和层析纯化获得高纯度蛋白。该蛋白成功用于检测肾小管间质性肾炎患者血清中的自身抗体，证实其在临床诊断中的应用潜力。
2. **文献名称**: *Structural Characterization of Human TINAG by Recombinant Protein Technology*
**作者**: Zhang L, et al.
**摘要**: 利用哺乳动物表达系统(HEK293细胞)制备重组TINAG蛋白，通过质谱和圆二色谱分析其糖基化修饰与空间结构，揭示了TINAG在肾小管基底膜中的潜在分子相互作用机制。
3. **文献名称**: *TINAG Recombinant Protein Induces Experimental Autoimmune Tubulointerstitial Nephritis in Mice*
**作者**: Tanaka K, et al.
**摘要**: 通过注射重组TINAG蛋白诱导小鼠模型，发现其可触发Th1/Th17免疫反应，导致肾间质炎症和纤维化，为研究自身免疫性肾病提供了实验工具。
4. **文献名称**: *Development of a TINAG-ELISA Kit Using Recombinant Antigen for Renal Disease Screening*
**作者**: Wang Y, et al.
**摘要**: 基于重组TINAG蛋白开发ELISA检测试剂盒，验证了其在慢性肾脏病患者中的敏感性和特异性，表明其可作为非侵入性生物标志物用于疾病监测。
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注：以上文献为模拟示例，实际研究中请通过PubMed或Web of Science等平台检索真实发表的论文。
**Background of TINAG Recombinant Protein**
TINAG (Tubulointerstitial Nephritis Antigen) is a glycoprotein primarily expressed in the kidney, specifically within the tubulointerstitial compartment. Initially identified as an autoantigen linked to tubulointerstitial nephritis (TIN), a condition characterized by inflammation and scarring of renal tubules, TINAG plays a role in maintaining kidney structure and function. It is a 58-60 kDa protein encoded by the *TINAG* gene and shares structural homology with extracellular matrix proteins, featuring multiple epidermal growth factor (EGF)-like domains and a cysteine-rich region, suggesting potential involvement in cell adhesion or signaling.
The recombinant form of TINAG is produced using biotechnological platforms, such as bacterial or mammalian expression systems, to enable detailed study of its biological properties. Recombinant TINAG has been instrumental in elucidating its interactions with immune components, particularly its role in autoimmune responses. Studies indicate that TINAG may act as a target for autoantibodies in certain kidney diseases, contributing to disease progression. Additionally, recombinant TINAG serves as a critical tool in diagnostic assays to detect anti-TINAG antibodies in patient sera, aiding in the diagnosis and monitoring of TIN and related renal disorders.
Research on recombinant TINAG also explores its potential therapeutic applications, including modulating immune responses or serving as a biomarker for early kidney injury. Despite progress, the precise molecular mechanisms of TINAG in renal pathophysiology remain under investigation, highlighting the need for further functional and clinical studies to fully harness its diagnostic and therapeutic potential.
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