| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DPP4 |
| Uniprot No | P27487 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 39-766aa |
| 氨基酸序列 | SRKTYTLTDYLK NTYRLKLYSL RWISDHEYLY KQENNILVFN AEYGNSSVFL ENSTFDEFGH SINDYSISPD GQFILLEYNY VKQWRHSYTA SYDIYDLNKR QLITEERIPN NTQWVTWSPV GHKLAYVWNN DIYVKIEPNL PSYRITWTGK EDIIYNGITD WVYEEEVFSA YSALWWSPNG TFLAYAQFND TEVPLIEYSF YSDESLQYPK TVRVPYPKAG AVNPTVKFFV VNTDSLSSVT NATSIQITAP ASMLIGDHYL CDVTWATQER ISLQWLRRIQ NYSVMDICDY DESSGRWNCL VARQHIEMST TGWVGRFRPS EPHFTLDGNS FYKIISNEEG YRHICYFQID KKDCTFITKG TWEVIGIEAL TSDYLYYISN EYKGMPGGRN LYKIQLSDYT KVTCLSCELN PERCQYYSVS FSKEAKYYQL RCSGPGLPLY TLHSSVNDKG LRVLEDNSAL DKMLQNVQMP SKKLDFIILN ETKFWYQMIL PPHFDKSKKY PLLLDVYAGP CSQKADTVFR LNWATYLAST ENIIVASFDG RGSGYQGDKI MHAINRRLGT FEVEDQIEAA RQFSKMGFVD NKRIAIWGWS YGGYVTSMVL GSGSGVFKCG IAVAPVSRWE YYDSVYTERY MGLPTPEDNL DHYRNSTVMS RAENFKQVEY LLIHGTADDN VHFQQSAQIS KALVDVGVDF QAMWYTDEDH GIASSTAHQH IYTHMSHFIK QCFSLP SGRLVPRGSHHHHHH |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DPP4重组蛋白的3篇参考文献概览(信息基于公开研究整理，非真实文献引用)：
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1. **文献名称**：*Expression and Characterization of Recombinant Human DPP4 in HEK293 Cells*
**作者**：Zhang Y. et al.
**摘要**：研究利用HEK293细胞系统高效表达重组人源DPP4蛋白，通过亲和层析纯化获得高纯度蛋白，并验证其酶活性与天然DPP4一致，为药物筛选提供可靠工具。
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2. **文献名称**：*Structural Insights into DPP4 Inhibition by Sitagliptin Using Recombinant Protein Crystallography*
**作者**：Smith J.R., Brown K.L.
**摘要**：通过重组表达DPP4胞外结构域并结合X射线晶体学技术，解析其与抑制剂西格列汀的复合物结构，揭示药物结合的关键位点，指导新型抑制剂设计。
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3. **文献名称**：*Optimization of DPP4 Recombinant Production in E. coli for Antidiabetic Studies*
**作者**：Lee S. et al.
**摘要**：优化大肠杆菌表达系统生产重组DPP4的工艺，解决包涵体复性难题，获得具有生物活性的蛋白，成功应用于体外降糖药物功效评估。
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注：以上为示例性内容，实际文献需通过学术数据库(如PubMed、ScienceDirect)检索确认。
Dipeptidyl peptidase 4 (DPP4), also known as CD26. is a multifunctional transmembrane glycoprotein belonging to the serine protease family. It is widely expressed in tissues, including the liver, kidney, intestines, and immune cells, and exists in both membrane-bound and soluble forms. DPP4 cleaves N-terminal dipeptides from substrates with proline or alanine residues, regulating the activity of peptides like glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP), which are critical for glucose metabolism. This enzymatic function has made DPP4 a key therapeutic target for type 2 diabetes, with inhibitors like sitagliptin enhancing insulin secretion by prolonging incretin activity.
Recombinant DPP4 proteins are engineered using expression systems such as mammalian (e.g., CHO cells), insect, or bacterial cells to produce purified, bioactive forms for research and drug development. These systems enable precise control over post-translational modifications, ensuring structural and functional fidelity. Recombinant DPP4 retains the native enzyme's homodimeric structure and catalytic activity, allowing its use in biochemical assays, inhibitor screening, and structural studies (e.g., X-ray crystallography).
Beyond diabetes, DPP4 interacts with cytokines, chemokines, and cell surface proteins, implicating it in immunomodulation, cancer progression, and viral entry—notably, some coronaviruses exploit DPP4 as a co-receptor. Recombinant DPP4 facilitates studies on these pathways and supports vaccine development, such as investigating its role in MERS-CoV infection. Its dual utility in basic science and translational applications underscores its importance in understanding disease mechanisms and advancing targeted therapies.
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