| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Hers |
| Uniprot No | P06737 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-846aa |
| 氨基酸序列 | AKPLTDQEKRRQISIRGIVGVENVAELKKSFNRHLHFTLVKDRNVATTRDYYFALAHTVRDHLVGRWIRTQQHYYDKCPKRVYYLSLEFYMGRTLQNTMINLGLQNACDEAIYQLGLDIEELEEIEEDAGLGNGGLGRLAACFLDSMATLGLAAYGYGIRYEYGIFNQKIRDGWQVEEADDWLRYGNPWEKSRPEFMLPVHFYGKVEHTNTGTKWIDTQVVLALPYDTPVPGYMNNTVNTMRLWSARAPNDFNLRDFNVGDYIQAVLDRNLAENISRVLYPNDNFFEGKELRLKQEYFVVAATLQDIIRRFKASKFGSTRGAGTVFDAFPDQVAIQLNDTHPALAIPELMRIFVDIEKLPWSKAWELTQKTFAYTNHTVLPEALERWPVDLVEKLLPRHLEIIYEINQKHLDRIVALFPKDVDRLRRMSLIEEEGSKRINMAHLCIVGSHAVNGVAKIHSDIVKTKVFKDFSELEPDKFQNKTNGITPRRWLLLCNPGLAELIAEKIGEDYVKDLSQLTKLHSFLGDDVFLRELAKVKQENKLKFSQFLETEYKVKINPSSMFDVQVKRIHEYKRQLLNCLHVITMYNRIKKDPKKLFVPRTVIIGGKAAPGYHMAKMIIKLITSVADVVNNDPMVGSKLKVIFLENYRVSLAEKVIPATDLSEQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGEENLFIFGMRIDDVAALDKKGYEAKEYYEALPELKLVIDQIDNGFFSPKQPDLFKDIINMLFYHDRFKVFADYEAYVKCQDKVSQLYMNPKAWNTMVLKNIAASGKFSSDRTIKEYAQNIWNVEPSDLKISLSNESNKVNG |
| 预测分子量 | 123.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与HER2(人类表皮生长因子受体2)重组蛋白相关的代表性文献摘要(经简化整理，非真实文献)：
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1. **"Use of chemotherapy plus a monoclonal antibody against HER2 for metastatic breast cancer that overexpresses HER2"**
- **作者**: Slamon, D.J. 等
- **摘要**: 研究验证了重组抗HER2单抗(赫赛汀)联合化疗对HER2阳性乳腺癌的疗效，证明其显著延长患者生存期，推动了靶向重组蛋白药物的临床应用。
2. **"Recombinant HER2 extracellular domain expression in E. coli for antibody production"**
- **作者**: Carter, P. 等
- **摘要**: 报道了通过大肠杆菌重组表达系统高效制备HER2胞外域蛋白，并用于筛选高亲和力抗体，为诊断和药物开发提供基础材料。
3. **"Structural basis for ligand-independent activation of HER2 by kinase domain dimerization"**
- **作者**: Yarden, Y. 等
- **摘要**: 通过重组HER2蛋白的晶体结构分析，揭示了HER2受体不依赖配体的二聚化激活机制，为设计抑制其信号通路的药物提供理论依据。
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注：以上文献标题和内容为示例性质，实际研究中建议通过PubMed或Google Scholar以关键词“HER2 recombinant protein”或“HER2 ectodomain”检索最新权威论文。
**Background of Hers Recombinant Protein**
Hers recombinant protein is a engineered biomolecule designed to mimic specific functional aspects of naturally occurring proteins, tailored for research, therapeutic, or diagnostic applications. The term "Hers" may refer to a protein associated with a particular gene, pathway, or proprietary technology, though its exact context depends on the research or commercial framework in which it is developed. Recombinant proteins like Hers are typically produced using biotechnological methods, where host systems such as *E. coli*, yeast, or mammalian cells are genetically modified to express the target protein. This allows for large-scale, consistent production with high purity.
The development of Hers recombinant protein likely stems from the need to study or modulate specific biological processes. For instance, if Hers is linked to a disease-related target (e.g., a receptor, enzyme, or signaling molecule), it could serve as a tool for drug discovery, structural biology, or cellular pathway analysis. Recombinant proteins are pivotal in therapeutics, such as monoclonal antibodies or enzyme replacement therapies, suggesting potential biomedical relevance for Hers.
Key features of Hers recombinant protein might include modifications like fusion tags (e.g., His-tag, GFP) for purification or tracking, post-translational modifications (e.g., glycosylation) achieved through mammalian expression systems, or optimized stability for *in vitro* or *in vivo* applications. Its characterization would involve assays validating activity, specificity, and interaction partners.
Overall, Hers recombinant protein represents a versatile tool in biotechnology, enabling advancements in basic research, biopharmaceutical development, and precision medicine. Its design and application align with the broader goals of recombinant protein technology: to provide scalable, reproducible, and functional molecules for solving complex biological challenges.
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