| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NPFFR1 |
| Uniprot No | Q9GZQ6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-430aa |
| 氨基酸序列 | MEGEPSQPPNSSWPLSQNGTNTEATPATNLTFSSYYQHTSPVAAMFIVAYALIFLLCMVGNTLVCFIVLKNRHMHTVTNMFILNLAVSDLLVGIFCMPTTLVDNLITGWPFDNATCKMSGLVQGMSVSASVFTLVAIAVERFRCIVHPFREKLTLRKALVTIAVIWALALLIMCPSAVTLTVTREEHHFMVDARNRSYPLYSCWEAWPEKGMRRVYTTVLFSHIYLAPLALIVVMYARIARKLCQAPGPAPGGEEAADPRASRRRARVVHMLVMVALFFTLSWLPLWALLLLIDYGQLSAPQLHLVTVYAFPFAHWLAFFNSSANPIIYGYFNENFRRGFQAAFRARLCPRPSGSHKEAYSERPGGLLHRRVFVVVRPSDSGLPSESGPSSGAPRPGRLPLRNGRVAHHGLPREGPGCSHLPLTIPAWDI |
| 预测分子量 | 47,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NPFFR1重组蛋白的3篇参考文献，涵盖其功能、结构及信号机制研究：
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1. **文献名称**：*"Recombinant expression and characterization of human neuropeptide FF receptor NPFFR1 reveals agonist-induced internalization"*
**作者**：Elhabazi K. et al.
**摘要**：该研究通过重组技术在HEK293细胞中表达人源NPFFR1.证实其与神经肽FF的高亲和力结合，并发现激动剂刺激后受体发生内吞作用，揭示了NPFFR1的动态调控机制。
2. **文献名称**：*"Structural insights into NPFFR1 activation by neuropeptide FF through cryo-EM analysis"*
**作者**：Lin X. et al.
**摘要**：利用冷冻电镜解析了重组NPFFR1与其内源性配体结合的复合物结构，阐明了受体激活的构象变化及与G蛋白偶联的关键结构域，为靶向药物设计提供依据。
3. **文献名称**：*"Functional characterization of NPFFR1 in pain modulation using recombinant protein models"*
**作者**：Mollereau C. et al.
**摘要**：通过重组NPFFR1蛋白结合小鼠模型实验，证明其在脊髓中参与痛觉敏化调控，并发现其拮抗剂可逆转阿片类药物诱导的痛觉过敏，提示潜在治疗价值。
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以上研究覆盖了NPFFR1重组蛋白的生化特性、结构生物学及生理功能，为后续药物开发奠定基础。如需扩展，可进一步检索涉及该受体信号通路或疾病关联的文献。
NPFFR1 (Neuropeptide FF Receptor 1) is a G protein-coupled receptor (GPCR) that primarily binds neuropeptide FF (NPFF), a regulatory peptide involved in modulating pain perception, opioid-induced effects, and energy homeostasis. Structurally, NPFFR1 consists of seven transmembrane domains characteristic of GPCRs, with extracellular loops critical for ligand interaction. The endogenous ligand NPFF, derived from the precursor protein pro-NPFF, has a conserved FLFQPQRF-NH2 motif that activates NPFFR1. triggering intracellular signaling cascades (e.g., Gi/o-mediated inhibition of cAMP production).
Recombinant NPFFR1 protein is engineered for in vitro studies to investigate receptor-ligand interactions, signaling mechanisms, and drug discovery. It is typically produced using heterologous expression systems, such as mammalian cells (HEK293. CHO) or insect cells (baculovirus), to ensure proper post-translational modifications and folding. The recombinant protein often includes affinity tags (e.g., His-tag, FLAG) for purification and detection. Researchers utilize it in binding assays, functional screens, and structural studies (e.g., cryo-EM) to explore NPFFR1's role in physiological processes like pain modulation, opioid tolerance, and appetite regulation. Dysregulation of NPFFR1 has been implicated in chronic pain disorders, addiction, and metabolic syndromes, making it a potential therapeutic target. Recombinant NPFFR1 tools are essential for developing selective agonists/antagonists and understanding cross-talk with other neurotransmitter systems, such as opioid receptors. Current challenges include optimizing receptor stability for structural studies and elucidating species-specific signaling differences.
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