| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NtnG2 |
| Uniprot No | Q96CW9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 18-507aa |
| 氨基酸序列 | DYD ICKSWVTTDE GPTWEFYACQ PKVMRLKDYV KVKVEPSGIT CGDPPERFCS HENPYLCSNE CDASNPDLAH PPRLMFDKEE EGLATYWQSI TWSRYPSPLE ANITLSWNKT VELTDDVVMT FEYGRPTVMV LEKSLDNGRT WQPYQFYAED CMEAFGMSAR RARDMSSSSA HRVLCTEEYS RWAGSKKEKH VRFEVRDRFA IFAGPDLRNM DNLYTRLESA KGLKEFFTLT DLRMRLLRPA LGGTYVQREN LYKYFYAISN IEVIGRCKCN LHANLCSMRE GSLQCECEHN TTGPDCGKCK KNFRTRSWRA GSYLPLPHGS PNACATAGSF GNCECYGHSN RCSYIDFLNV VTCVSCKHNT RGQHCQHCRL GYYRNGSAEL DDENVCIECN CNQIGSVHDR CNETGFCECR EGAAGPKCDD CLPTHYWRQG CYPNVCDDDQ LLCQNGGTCL QNQRCACPRG YTGVRCEQPR CDPADDDGGL DCDRAPG |
| 预测分子量 | 59,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NtnG2重组蛋白研究的示例参考文献(内容为模拟概括，实际文献需根据具体研究补充)：
---
1. **标题**：*Expression and Functional Analysis of Recombinant Netrin-G2 in Neural Development*
**作者**：Smith A, et al.
**摘要**：本研究通过哺乳动物细胞表达系统成功制备了重组NtnG2蛋白，并验证其与突触后膜蛋白的相互作用，揭示了NtnG2在神经元轴突导向中的关键作用。
2. **标题**：*Purification and Structural Characterization of NTNG2 Recombinant Protein*
**作者**：Lee H, Kim J.
**摘要**：利用大肠杆菌原核表达系统纯化NTNG2重组蛋白，通过X射线晶体学解析其三维结构，为研究其与配体结合机制提供了结构基础。
3. **标题**：*Netrin-G2 Recombinant Protein Promotes Synaptic Plasticity in Vitro*
**作者**：Zhang Q, et al.
**摘要**：通过HEK293细胞表达重组Netrin-G2.发现其可增强海马神经元突触可塑性，提示其在神经退行性疾病治疗中的潜在应用价值。
---
**说明**：
- NtnG2(Netrin-G2)属于Netrin蛋白家族，主要参与神经系统发育及突触形成。
- 以上文献示例聚焦重组蛋白的表达、结构解析及功能研究，实际文献需通过PubMed或Google Scholar以关键词“Netrin-G2 recombinant”检索。
**Background of NtnG2 Recombinant Protein**
NtnG2 is a recombinant protein belonging to the N-terminal nucleophile (Ntn) hydrolase superfamily, a class of enzymes characterized by their unique αββα fold and catalytic mechanism involving a nucleophilic residue at the N-terminus. These enzymes are widely distributed across prokaryotes and eukaryotes, playing critical roles in amino acid metabolism, protein degradation, and biosynthesis of bioactive molecules. NtnG2. in particular, is engineered through recombinant DNA technology, where its gene is cloned, expressed, and purified in heterologous systems like *E. coli* or mammalian cell cultures to ensure high yield and purity for functional studies.
The interest in NtnG2 stems from its potential applications in biocatalysis and therapeutic development. As an Ntn hydrolase, it exhibits β-aminopeptidase activity, enabling selective cleavage of peptide bonds, which is valuable for industrial processes such as chiral compound synthesis or peptide drug modification. Structurally, recombinant NtnG2 retains the conserved catalytic triad (typically involving Thr/Ser as the nucleophile) and substrate-binding pockets, but its engineered form often incorporates modifications to enhance stability, solubility, or substrate specificity compared to wild-type counterparts.
Research on NtnG2 also explores its role in disease contexts. For instance, homologs of Ntn hydrolases are implicated in cancer progression or antibiotic resistance, making NtnG2 a candidate for structure-function studies to design inhibitors or biosensors. Additionally, recombinant NtnG2 serves as a tool to dissect enzymatic mechanisms via mutagenesis or crystallography, providing insights into substrate recognition and catalysis.
Overall, NtnG2 exemplifies the intersection of protein engineering and enzymology, offering a versatile platform for both industrial and biomedical innovation. Its study contributes to advancing synthetic biology and targeted therapeutics, underscoring the importance of recombinant technologies in modern biotechnology.
×
