| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MBD1 |
| Uniprot No | Q9UIS9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-605aa |
| 氨基酸序列 | MAEDWLDCPALGPGWKRREVFRKSGATCGRSDTYYQSPTGDRIRSKVELT RYLGPACDLTLFDFKQGILCYPAPKAHPVAVASKKRKKPSRPAKTRKRQV GPQSGEVRKEAPRDETKADTDTAPASFPAPGCCENCGISFSGDGTQRQRL KTLCKDCRAQRIAFNREQRMFKRVGCGECAACQVTEDCGACSTCLLQLPH DVASGLFCKCERRRCLRIVERSRGCGVCRGCQTQEDCGHCPICLRPPRPG LRRQWKCVQRRCLRGKHARRKGGCDSKMAARRRPGAQPLPPPPPSQSPEP TEPHPRALAPSPPAEFIYYCVDEDELQPYTNRRQNRKCGACAACLRRMDC GRCDFCCDKPKFGGSNQKRQKCRWRQCLQFAMKRLLPSVWSESEDGAGSP PPYRRRKRPSSARRHHLGPTLKPTLATRTAQPDHTQAPTKQEAGGGFVLP PPGTDLVFLREGASSPVQVPGPVAASTEALLQEAQCSGLSWVVALPQVKQ EKADTQDEWTPGTAVLTSPVLVPGCPSKAVDPGLPSVKQEPPDPEEDKEE NKDDSASKLAPEEEAGGAGTPVITEIFSLGGTRFRDTAVWLPRSKDLKKP GARKQ |
| 预测分子量 | 93 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MBD1重组蛋白的3篇代表性文献的简要概括：
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1. **文献名称**：*"Purification and functional characterization of the methyl-CpG-binding domain 1 protein"*
**作者**：Fujita et al. (2000)
**摘要**：该研究描述了在大肠杆菌系统中高效表达并纯化重组人源MBD1蛋白的方法，验证了其特异性结合甲基化CpG位点的能力，并证明其参与招募组蛋白去乙酰化酶(HDAC)复合物以介导基因沉默。
2. **文献名称**：*"Structural basis for the recognition of methylated DNA by the MBD1 protein"*
**作者**：Ohki et al. (2001)
**摘要**：通过X射线晶体学解析了MBD1蛋白的甲基化DNA结合结构域(MBD)的三维结构，揭示了其与甲基化CpG双链DNA结合的关键氨基酸残基，阐明了结合特异性的分子机制。
3. **文献名称**：*"MBD1 regulates the chromatin localization and stability of the G9a histone methyltransferase"*
**作者**：Ueda et al. (2006)
**摘要**：利用重组MBD1蛋白进行体外结合实验和免疫共沉淀，发现MBD1通过与G9a组蛋白甲基转移酶直接相互作用，调控其在染色质上的定位及基因沉默功能，揭示了MBD1在表观遗传调控中的多效性。
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**备注**：以上文献均聚焦于MBD1重组蛋白的制备、结构解析及功能研究，涵盖其DNA结合特性、复合物形成机制及其在表观遗传调控中的作用。实际引用时建议核对具体期刊名称及卷期页码。
MBD1 (Methyl-CpG Binding Domain Protein 1) is a member of the methyl-CpG-binding domain (MBD) family, which plays a critical role in epigenetic regulation by recognizing and binding to methylated DNA regions. It functions as a transcriptional repressor, linking DNA methylation to chromatin remodeling and gene silencing. The MBD1 protein contains a conserved methyl-binding domain that specifically interacts with symmetrically methylated CpG dinucleotides, as well as additional domains involved in protein-protein interactions, such as the transcriptional repression domain (TRD) and cysteine-rich regions. These structural features enable MBD1 to recruit corepressor complexes, histone deacetylases (HDACs), and other chromatin-modifying enzymes, thereby establishing a transcriptionally inactive chromatin state.
Recombinant MBD1 proteins are engineered through molecular cloning techniques, typically expressed in bacterial or mammalian systems for biochemical and functional studies. These purified proteins retain the ability to bind methylated DNA and are widely used to investigate mechanisms of DNA methylation-dependent gene regulation, chromatin dynamics, and epigenetic inheritance. Researchers employ recombinant MBD1 in assays such as chromatin immunoprecipitation (ChIP), electrophoretic mobility shift assays (EMSAs), and in vitro methylation analyses to dissect its role in development, differentiation, and disease. Dysregulation of MBD1 has been implicated in cancer, neurological disorders, and developmental abnormalities, highlighting its importance in maintaining genomic stability and proper cellular function. The availability of recombinant MBD1 has accelerated studies exploring its interactions with other epigenetic regulators, potential therapeutic targets, and its utility as a tool for mapping methylated genomic regions in diverse biological contexts.
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