| 纯度 | >90%SDS-PAGE. |
| 种属 | Rat |
| 靶点 | CYP2F2 |
| Uniprot No | O35293 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-491aa |
| 氨基酸序列 | MDGVSTAILL LLLAVISLSL TFTSWGKGQL PPGPKPLPIL GNLLQLRSQD LLTSLTKLSK DYGSVFTVYL GPRRVIVLSG YQTVKEALVD KGEEFSGRGS YPIFFNFTKG NGIAFSDGER WKILRRFSVQ ILRNFGMGKR SIEERILEEG SFLLDVLRKT EGKPFDPVFI LSRSVSNIIC SVIFGSRFDY DDERLLTIIH FINDNFQIMS SPWGEMYNIF PSLLDWVPGP HRRVFRNFGG MKDLIARSVR EHQDSLDPNS PRDFIDCFLT KMVQEKQDPL SHFNMDTLLM TTHNLLFGGT ETVGTTLRHA FLILMKYPKV QARVQEEIDC VVGRSRMPTL EDRASMPYTD AVIHEVQRFA DVIPMNLPHR VIRDTPFRGF LIPKGTDVIT LLNTVHYDSD QFKTPQEFNP EHFLDANQSF KKSPAFMPFS AGRRLCLGEP LARMELFIYL TSILQNFTLH PLVEPEDIDL TPLSSGLGNL PRPFQLCMRI R |
| 预测分子量 | 55,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CYP2F2重组蛋白的参考文献及简要摘要：
1. **"Cloning, expression, and characterization of CYP2F2: A lung-selective cytochrome P450"**
*He XY et al. (2001)*
该研究成功在大肠杆菌中重组表达了人CYP2F2蛋白，并验证其在萘代谢中的活性，强调其在肺组织特异性毒性代谢中的作用。
2. **"Metabolic activation of naphthalene by human CYP2F2 in recombinant systems"**
*Dong J et al. (2008)*
通过昆虫细胞表达系统获得活性CYP2F2重组蛋白，证明其能代谢萘生成毒性中间体，揭示了该酶在吸入性毒素致肺损伤中的潜在机制。
3. **"Substrate specificity and kinetic properties of recombinant CYP2F2"**
*Lanza DL et al. (1999)*
利用杆状病毒系统表达CYP2F2.分析其对多环芳烃的代谢偏好性及酶动力学参数，发现其对小分子烃类化合物具有高亲和力。
4. **"Structural insights into CYP2F2-mediated bioactivation of pulmonary toxicants"**
*Yost GS et al. (2005)*
结合分子对接和突变研究，探讨CYP2F2重组蛋白的活性位点结构，阐明其如何特异性激活肺部毒素(如萘)为反应性代谢物。
这些研究覆盖了CYP2F2重组蛋白的表达策略、代谢功能及结构机制，为理解其在肺毒性中的作用提供了基础。
CYP2F2. a member of the cytochrome P450 superfamily, is a membrane-bound monooxygenase enzyme primarily involved in the metabolism of endogenous and exogenous compounds. It is predominantly expressed in the lung and liver tissues of certain species, including mice, and plays a critical role in the bioactivation of toxicants and procarcinogens. Unlike some human P450 isoforms (e.g., CYP2A13), CYP2F2 exhibits species-specific expression patterns, making it a valuable model for studying pulmonary xenobiotic metabolism and toxicity mechanisms. Its enzymatic activity is linked to the metabolic activation of environmental pollutants like naphthalene, styrene, and 3-methylindole, which can cause lung injury through the generation of reactive intermediates.
Recombinant CYP2F2 protein is engineered using heterologous expression systems such as Escherichia coli or insect cell lines (e.g., baculovirus/Sf9), often co-expressed with NADPH-cytochrome P450 reductase to restore catalytic functionality. This recombinant form enables detailed in vitro studies on substrate specificity, enzyme kinetics, and inhibitor interactions without interference from other metabolic pathways present in vivo. Structural and functional analyses of recombinant CYP2F2 have provided insights into its unique substrate-binding pocket architecture, explaining its preference for small hydrophobic molecules. Researchers also utilize this protein to investigate interspecies differences in toxicant metabolism, particularly relevant for extrapolating rodent-based toxicology data to human risk assessment. Additionally, CYP2F2 serves as a tool for identifying potential drug candidates that might undergo bioactivation into toxic metabolites, contributing to safety evaluations during pharmaceutical development. Its study remains crucial for understanding chemical-induced pulmonary damage and optimizing predictive toxicological models.
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