| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NXPH4 |
| Uniprot No | O95158 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-308aa |
| 氨基酸序列 | QIPESGR PQYLGLRPAA AGAGAPGQQL PEPRSSDGLG VGRAWSWAWP TNHTGALARA GAAGALPAQR TKRKPSIKAA RAKKIFGWGD FYFRVHTLKF SLLVTGKIVD HVNGTFSVYF RHNSSSLGNL SVSIVPPSKR VEFGGVWLPG PVPHPLQSTL ALEGVLPGLG PPLGMAAAAA GPGLGGSLGG ALAGPLGGAL GVPGAKESRA FNCHVEYEKT NRARKHRPCL YDPSQVCFTE HTQSQAAWLC AKPFKVICIF VSFLSFDYKL VQKVCPDYNF QSEHPYFG |
| 预测分子量 | 33 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NXPH4重组蛋白的假设性参考文献示例(具体文献需通过学术数据库核实)：
1. **文献名称**: "Neurexophilin 4 promotes excitatory synapse assembly through binding to α-neurexin"
**作者**: Smith A, et al.
**摘要**: 研究报道了重组NXPH4蛋白通过结合α-neurexin调控兴奋性突触形成，并验证了其在体外神经元共培养系统中的功能。
2. **文献名称**: "Structural characterization of recombinant NXPH4 and its interaction with neuroligin"
**作者**: Brown L, et al.
**摘要**: 通过X射线晶体学解析了NXPH4重组蛋白的三维结构，揭示了其与neuroligin-3的结合界面，暗示其在突触黏附中的作用。
3. **文献名称**: "Functional expression of NXPH4 in mammalian cells and its role in synaptic plasticity"
**作者**: Chen Q, et al.
**摘要**: 利用HEK293细胞系统表达重组NXPH4.发现其过表达增强海马神经元的长时程增强(LTP)，提示其参与突触可塑性调控。
4. **文献名称**: "NXPH4 recombinant protein alleviates synaptic deficits in a mouse model of autism spectrum disorder"
**作者**: Wang Y, et al.
**摘要**: 动物实验表明，外源性重组NXPH4蛋白可改善Shank3敲除小鼠的社交行为异常和突触传递缺陷。
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**注意**：以上为模拟示例，实际文献需通过PubMed、Web of Science等平台检索关键词"NXPH4 recombinant protein"或"neurexophilin 4"获取。建议结合研究领域(如神经科学、蛋白工程)筛选近年高相关度论文。
**Background of NXPH4 Recombinant Protein**
NXPH4 (Neurexophilin 4) is a member of the neurexophilin family, a group of small, secreted glycoproteins that interact with neurexins, transmembrane proteins critical for synaptic organization and neurotransmission. These proteins play roles in establishing and maintaining neural circuits by mediating cell adhesion and signaling at synapses. NXPH4 is encoded by the *NXPH4* gene and is expressed predominantly in the central nervous system, with emerging evidence of its involvement in neurodevelopmental processes.
Recombinant NXPH4 is produced using engineered expression systems (e.g., mammalian, insect, or bacterial cells) to ensure proper post-translational modifications, such as glycosylation, which are essential for its functional interactions. Structurally, it contains conserved domains characteristic of neurexophilins, including a signal peptide for secretion, a variable N-terminal region, and a C-terminal domain that binds to α-neurexins. This interaction is thought to stabilize synaptic connections and modulate neurotransmitter release.
Research on NXPH4 has highlighted its potential role in synaptic plasticity, neural circuit assembly, and behavior. Studies in model organisms suggest that disruptions in NXPH4-neurexin interactions may contribute to neuropsychiatric disorders, such as autism spectrum disorders or schizophrenia, though mechanistic details remain under investigation. Recombinant NXPH4 is widely used in *in vitro* assays to study protein-protein interactions, neuronal development, and synaptic function. It also serves as a tool for generating antibodies, screening therapeutic compounds, or exploring molecular pathways linked to neurological diseases.
Overall, NXPH4 recombinant protein provides a valuable resource for dissecting the molecular basis of synaptic biology and its implications in health and disease.
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