| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ATP2B4 |
| Uniprot No | P23634 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1241aa |
| 氨基酸序列 | MTNPSDRVLPANSMAESREGDFGCTVMELRKLMELRSRDALTQINVHYGGVQNLCSRLKTSPVEGLSGNPADLEKRRQVFGHNVIPPKKPKTFLELVWEALQDVTLIILEIAAIISLVLSFYRPAGEENELCGQVATTPEDENEAQAGWIEGAAILFSVIIVVLVTAFNDWSKEKQFRGLQCRIEQEQKFSIIRNGQLIQLPVAEIVVGDIAQVKYGDLLPADGILIQGNDLKIDESSLTGESDHVKKSLDKDPMLLSGTHVMEGSGRMVVTAVGVNSQTGIILTLLGVNEDDEGEKKKKGKKQGVPENRNKAKTQDGVALEIQPLNSQEGIDNEEKDKKAVKVPKKEKSVLQGKLTRLAVQIGKAGLLMSALTVFILILYFVIDNFVINRRPWLPECTPIYIQYFVKFFIIGITVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQAYIGGIHYRQIPSPDVFLPKVLDLIVNGISINSAYTSKILPPEKEGGLPRQVGNKTECALLGFVTDLKQDYQAVRNEVPEEKLYKVYTFNSVRKSMSTVIRNPNGGFRMYSKGASEIILRKCNRILDRKGEAVPFKNKDRDDMVRTVIEPMACDGLRTICIAYRDFDDTEPSWDNENEILTELTCIAVVGIEDPVRPEVPDAIAKCKQAGITVRMVTGDNINTARAIATKCGILTPGDDFLCLEGKEFNRLIRNEKGEVEQEKLDKIWPKLRVLARSSPTDKHTLVKGIIDSTVGEHRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTESLLKRRPYGRNKPLISRTMMKNILGHAFYQLIVIFILVFAGEKFFDIDSGRKAPLHSPPSQHYTIVFNTFVLMQLFNEINSRKIHGEKNVFSGIYRNIIFCSVVLGTFICQIFIVEFGGKPFSCTSLSLSQWLWCLFIGIGELLWGQFISAIPTRSLKFLKEAGHGTTKEEITKDAEGLDEIDHAEMELRRGQILWFRGLNRIQTQIDVINTFQTGASFKGVLRRQNMGQHLDVKLVPSSSYIKVVKAFHSSLHESIQKPYNQKSIHSFMTHPEFAIEEELPRTPLLDEEEEENPDKASKFGTRVLLLDGEVTPYANTNNNAVDCNQVQLPQSDSSLQSLETSV |
| 预测分子量 | 137 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ATP2B4(PMCA4)重组蛋白的3篇代表性文献示例：
1. **文献名称**：*"Functional characterization of recombinant human PMCA4b expressed in Sf9 insect cells"*
**作者**：Di Leva, F., et al.
**摘要**：本研究通过杆状病毒系统在Sf9昆虫细胞中表达了ATP2B4(PMCA4b)重组蛋白，并验证了其钙离子转运活性。通过生化分析发现，该重组蛋白的酶动力学特性与天然蛋白一致，为后续结构功能研究提供了可靠工具。
2. **文献名称**：*"Structural insights into the plasma membrane Ca²⁺ pump PMCA4 in complex with calmodulin"*
**作者**：Toyoshima, C., et al.
**摘要**：利用冷冻电镜技术解析了PMCA4重组蛋白与钙调蛋白(Calmodulin)复合物的高分辨率结构，揭示了PMCA4钙依赖性自抑制的分子机制，为靶向该蛋白的药物设计提供了结构基础。
3. **文献名称**：*"PMCA4 regulates angiogenesis through nitric oxide signaling in endothelial cells"*
**作者**：Schuh, K., et al.
**摘要**：通过重组PMCA4蛋白的体外实验，证明其通过调控内皮细胞钙稳态影响一氧化氮(NO)合成，进而调节血管生成，提示PMCA4可能成为治疗缺血性疾病的潜在靶点。
4. **文献名称**：*"High-throughput screening of PMCA4 inhibitors using a fluorescent calcium assay"*
**作者**：Strehler, E.E., et al.
**摘要**：开发了基于PMCA4重组蛋白的荧光钙离子检测方法，用于高通量筛选小分子抑制剂，发现了多个可调节PMCA4活性的先导化合物，推动心血管疾病治疗研究。
(注：以上文献为示例，实际引用时需核实具体来源及内容。)
ATP2B4. also known as plasma membrane calcium-transporting ATPase 4 (PMCA4), is a member of the P-type ATPase superfamily responsible for calcium ion transport across cell membranes. This protein plays a critical role in maintaining intracellular calcium homeostasis by actively extruding calcium from the cytoplasm, thereby regulating cellular processes such as signal transduction, muscle contraction, and neuronal activity. ATP2B4 is widely expressed, with notable abundance in erythrocytes, endothelial cells, and cardiac tissue. Its dysfunction has been linked to cardiovascular diseases, including hypertension, due to altered calcium handling in vascular smooth muscle cells.
Recombinant ATP2B4 protein is engineered through heterologous expression systems, such as mammalian or insect cell lines, to ensure proper post-translational modifications and functional activity. Purification typically involves affinity tags (e.g., His-tag) followed by chromatography, yielding a protein suitable for structural and functional studies. Researchers utilize recombinant ATP2B4 to investigate its enzymatic kinetics, calcium-binding properties, and interactions with regulatory partners like calmodulin. Structural analyses (e.g., cryo-EM) have provided insights into its autoinhibitory mechanisms and calcium transport cycle.
In disease contexts, ATP2B4 variants are associated with malaria resistance in African populations, highlighting its role in erythrocyte membrane integrity. Additionally, its involvement in nitric oxide synthase regulation positions it as a therapeutic target for cardiovascular disorders. Recombinant ATP2B4 also serves as an antigen for antibody development and a tool for high-throughput drug screening to identify modulators of calcium signaling pathways. Ongoing research aims to clarify its tissue-specific roles and potential in precision medicine for calcium-related pathologies.
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