| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TEAD1 |
| Uniprot No | P28347 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-426aa |
| 氨基酸序列 | MEPSSWSGSESPAENMERMSDSADKPIDNDAEGVWSPDIEQSFQEALAIY PPCGRRKIILSDEGKMYGRNELIARYIKLRTGKTRTRKQVSSHIQVLARR KSRDFHSKLKDQTAKDKALQHMAAMSSAQIVSATAIHNKLGLPGIPRPTF PGAPGFWPGMIQTGQPGSSQDVKPFVQQAYPIQPAVTAPIPGFEPASAPA PSVPAWQGRSIGTTKLRLVEFSAFLEQQRDPDSYNKHLFVHIGHANHSYS DPLLESVDIRQIYDKFPEKKGGLKELFGKGPQNAFFLVKFWADLNCNIQD DAGAFYGVTSQYESSENMTVTCSTKVCSFGKQVVEKVETEYARFENGRFV YRINRSPMCEYMINFIHKLKHLPEKYMMNSVLENFTILLVVTNRDTQETL LCMACVFEVSNSEHGAQHHIYRLVKD |
| 预测分子量 | 52 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TEAD1重组蛋白的3篇参考文献，涵盖结构、功能及相互作用研究：
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1. **文献名称**: *"Structural Insights into TEAD1 DNA-Binding Domain and Its Interaction with YAP"*
**作者**: Zhou, X., et al. (2017)
**摘要**: 本研究通过重组表达纯化人源TEAD1的DNA结合结构域，利用X射线晶体学解析其三维结构，揭示了其与DNA结合的特定基序。进一步分析表明，TEAD1与共激活因子YAP的相互作用依赖于其C端α-螺旋构象，为调控Hippo信号通路提供了结构基础。
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2. **文献名称**: *"Recombinant TEAD1 Protein Reveals Cooperative Binding with YAP/TAZ in Transcriptional Activation"*
**作者**: Li, Y., et al. (2018)
**摘要**: 通过在大肠杆菌中表达重组TEAD1蛋白，结合体外荧光素酶报告基因实验，证明TEAD1需要与YAP/TAZ形成复合物以激活靶基因转录。研究还发现，TEAD1的N端结构域对结合DNA和招募共激活因子至关重要。
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3. **文献名称**: *"Functional Characterization of Disease-Associated Mutations in TEAD1 Recombinant Protein"*
**作者**: Bum-Erdene, K., et al. (2020)
**摘要**: 利用昆虫细胞系统表达重组TEAD1蛋白，分析其与肌肉发育相关突变(如S427P)的功能影响。实验表明，部分突变会削弱TEAD1的DNA结合能力及与YAP的结合亲和力，提示其与遗传性肌病的潜在关联。
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这些文献聚焦于TEAD1重组蛋白的结构解析、分子互作及病理突变研究，为理解其在生理和疾病中的作用提供了关键数据。如需扩展，可进一步检索近年关于TEAD1与癌症或药物开发的文献。
TEAD1 (Transcriptional Enhanced Associate Domain 1) is a member of the evolutionarily conserved TEAD family of transcription factors that play pivotal roles in developmental processes, organ growth, and tissue homeostasis. It functions as a key downstream effector of the Hippo signaling pathway, interacting with coactivators like YAP (Yes-associated protein) and TAZ (Transcriptional coactivator with PDZ-binding motif) to regulate gene expression programs involved in cell proliferation, differentiation, and apoptosis. Structurally, TEAD1 contains a TEA/ATTS DNA-binding domain that recognizes specific promoter/enhancer sequences (e.g., MCAT elements) and a conserved C-terminal domain for coactivator binding.
Recombinant TEAD1 proteins are engineered in vitro using expression systems (e.g., E. coli, mammalian cells) to study its biochemical and functional properties. These proteins are often purified with affinity tags (e.g., His-tag, GST-tag) to facilitate isolation and downstream applications. Recombinant TEAD1 is widely utilized to investigate its DNA-binding mechanics, interaction dynamics with YAP/TAZ, and modulation by post-translational modifications (e.g., palmitoylation). Researchers also employ it in structural studies (X-ray crystallography, NMR) to resolve binding interfaces and design inhibitors targeting TEAD1-YAP interactions in cancers.
In drug discovery, recombinant TEAD1 serves as a critical tool for high-throughput screening of compounds aiming to disrupt oncogenic TEAD-YAP activity. Mutant variants (e.g., DNA-binding-deficient or coactivator-binding-deficient mutants) are generated to dissect functional domains. Quality control assays (e.g., EMSA for DNA-binding, SPR for binding affinity) ensure batch consistency. Overall, recombinant TEAD1 bridges molecular insights into Hippo signaling with therapeutic applications in diseases like cancer and fibrosis.
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