| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DYNLRB1 |
| Uniprot No | Q9NP97 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-96aa |
| 氨基酸序列 | MAEVEETLKR LQSQKGVQGI IVVNTEGIPI KSTMDNPTTT QYASLMHSFI LKARSTVRDI DPQNDLTFLR IRSKKNEIMV APDKDYFLIVIQNPTELEHH HHHH |
| 预测分子量 | 12 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DYNLRB1重组蛋白的模拟参考文献示例(实际文献需通过学术数据库验证)：
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1. **文献名称**: *Crystal structure of human dynein light chain DYNLRB1 and its interaction with the intermediate chain*
**作者**: Li, X., et al.
**摘要**: 解析了DYNLRB1重组蛋白的晶体结构，揭示了其与动力蛋白中间链(DYNCI)的结合界面，为动力蛋白复合物的组装机制提供了结构基础。
2. **文献名称**: *DYNLRB1 regulates mitotic spindle orientation through binding to NuMA in mammalian cells*
**作者**: Wang, Y., et al.
**摘要**: 通过重组DYNLRB1蛋白的体外结合实验，证明其与核有丝分裂器蛋白(NuMA)互作，调控细胞分裂过程中纺锤体的定向，敲低DYNLRB1导致有丝分裂缺陷。
3. **文献名称**: *Recombinant DYNLRB1 expression and functional analysis in neuronal cargo transport*
**作者**: Tanaka, K., et al.
**摘要**: 在大肠杆菌中成功表达并纯化重组DYNLRB1蛋白，发现其缺失会损害神经元轴突运输中动力蛋白驱动的逆向囊泡运输，提示其在神经退行性疾病中的潜在作用。
4. **文献名称**: *DYNLRB1 overexpression correlates with poor prognosis in colorectal cancer*
**作者**: Chen, L., et al.
**摘要**: 利用重组DYNLRB1蛋白制备抗体，分析结直肠癌组织样本，发现DYNLRB1高表达与肿瘤转移和患者生存率降低相关，可能作为预后标志物。
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建议通过 **PubMed/Google Scholar** 搜索关键词“DYNLRB1 recombinant”或“Dynein light chain RB1”获取真实文献。
DYNLRB1 (dynein light chain roadblock-type 1) is a key component of the cytoplasmic dynein motor complex, a molecular machine responsible for minus-end-directed microtubule-based intracellular transport. As a light chain subunit, it plays a regulatory role in dynein-mediated cargo trafficking, organelle positioning, and mitotic processes. The recombinant DYNLRB1 protein, typically produced in E. coli or mammalian expression systems, retains the conserved structural features of the native protein, including a helical-rich domain that facilitates interactions with dynein intermediate chains and cargo adaptors.
Studies highlight its involvement in Golgi apparatus organization, endosome-lysosome trafficking, and spindle orientation during cell division. DYNLRB1 binds specifically to components like BICD2 (Bicaudal D homolog 2), linking dynein to diverse cellular cargoes. Dysregulation of DYNLRB1 has been associated with neurodegenerative diseases and cancer progression, where altered intracellular transport contributes to pathological states.
Recombinant DYNLRB1 serves as a critical tool for investigating dynein regulation mechanisms through in vitro binding assays, structural studies (e.g., X-ray crystallography), and functional rescue experiments in knockout models. Its post-translational modifications, particularly phosphorylation at conserved residues, are actively studied for their impact on dynein activity during cell cycle transitions. Recent research also explores its potential as a biomarker in diseases characterized by vesicular transport defects, making it a protein of both basic and translational interest.
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