| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SMDT1 |
| Uniprot No | Q9H4I9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-107aa |
| 氨基酸序列 | MASGAARWLVLAPVRSGALRSGPSLRKDGDVSAAWSGSGRSLVPSRSVIVTRSGAILPKPVKMSFGLLRVFSIVIPFLYVGTLISKNFAALLEEHDIFVPEDDDDDD |
| 预测分子量 | 11,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SMDT1重组蛋白的模拟参考文献示例(注：以下内容为学术假设性示例，实际文献需通过数据库查询确认)：
1. **文献名称**: "Structural and functional characterization of SMDT1 in mitochondrial calcium uptake"
**作者**: Li X, Wang Y, Cheng J, et al.
**摘要**: 本研究解析了SMDT1重组蛋白的晶体结构，证实其作为线粒体钙转运复合物的关键组分，通过与MICU1互作调控钙离子跨线粒体内膜运输，揭示了其在细胞能量代谢中的作用机制。
2. **文献名称**: "SMDT1 deficiency impairs mitochondrial calcium homeostasis and promotes apoptosis in mammalian cells"
**作者**: Zhang R, Chen L, Liu S, et al.
**摘要**: 通过构建SMDT1敲除细胞模型，发现SMDT1缺失导致线粒体钙摄取能力下降，进而引发内质网应激和细胞凋亡，提示其在维持细胞存活中的必要性。
3. **文献名称**: "Recombinant SMDT1 expression in E. coli and its application in studying MICU1 interaction"
**作者**: Gupta A, Patel K, Singh M.
**摘要**: 报道了SMDT1重组蛋白在大肠杆菌中的高效表达与纯化方法，并利用表面等离子体共振(SPR)验证其与MICU1蛋白的强结合能力，为相关药物筛选提供了工具。
4. **文献名称**: "The role of SMDT1 in neurodegenerative diseases: Insights from recombinant protein-based assays"
**作者**: Müller T, Schmidt B, Fischer P.
**摘要**: 通过体外实验发现，SMDT1重组蛋白异常表达会扰乱神经元线粒体钙稳态，可能与阿尔茨海默病中线粒体功能障碍相关，为疾病机制研究提供新方向。
(注：以上文献信息为示例性质，实际引用需根据真实发表文献补充完整信息。)
SMDT1 (Single-pass Membrane protein with Aspartate-rich Tail 1), also known as Mitochondrial Fission Regulator 1 (MFR1) or FEM1B, is a mitochondrial inner membrane protein involved in regulating mitochondrial dynamics and cellular energy metabolism. Initially identified as a scaffold protein interacting with the mitochondrial fission factor Drp1 (Dynamin-related protein 1), SMDT1 plays a critical role in maintaining mitochondrial morphology, cristae structure, and apoptosis. It is encoded by the *SMDT1* gene located on human chromosome 22q13.2.
Structurally, SMDT1 contains a single transmembrane domain near its N-terminus and a C-terminal aspartate-rich tail, which mediates interactions with other mitochondrial proteins. Studies suggest that SMDT1 regulates the oligomerization of the mitochondrial permeability transition pore (mPTP) by binding to cyclophilin D (CypD) and adenine nucleotide translocase (ANT), influencing calcium homeostasis and mitochondrial membrane potential. This interaction links SMDT1 to processes like programmed cell death and ischemia-reperfusion injury.
Recombinant SMDT1 protein is engineered to study its biochemical functions, structural properties, and interactions with partners like MIEF1 (Mitochondrial Elongation Factor 1) or Drp1. It is typically expressed in prokaryotic (e.g., *E. coli*) or eukaryotic systems (e.g., HEK293 cells) with affinity tags (e.g., His-tag) for purification. Applications include in vitro binding assays, structural analysis (e.g., crystallography), and drug screening targeting mitochondrial dysfunction-related diseases such as neurodegenerative disorders, cardiovascular diseases, and cancer. Recent research also explores its role in mitophagy and metabolic reprogramming, highlighting its therapeutic potential.
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