| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TAS1R3 |
| Uniprot No | Q7RTX0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-570aa |
| 氨基酸序列 | PLCLSQQLRMKGDYVLGGLFPLGEAEEAGLRSRTRPSSPVCTRFSSNGLLWALAMKMAVEEINNKSDLLPGLRLGYDLFDTCSEPVVAMKPSLMFLAKAGSRDIAAYCNYTQYQPRVLAVIGPHSSELAMVTGKFFSFFLMPQVSYGASMELLSARETFPSFFRTVPSDRVQLTAAAELLQEFGWNWVAALGSDDEYGRQGLSIFSALAAARGICIAHEGLVPLPRADDSRLGKVQDVLHQVNQSSVQVVLLFASVHAAHALFNYSISSRLSPKVWVASEAWLTSDLVMGLPGMAQMGTVLGFLQRGAQLHEFPQYVKTHLALATDPAFCSALGEREQGLEEDVVGQRCPQCDCITLQNVSAGLNHHQTFSVYAAVYSVAQALHNTLQCNASGCPAQDPVKPWQLLENMYNLTFHVGGLPLRFDSSGNVDMEYDLKLWVWQGSVPRLHDVGRFNGSLRTERLKIRWHTSDNQKPVSRCSRQCQEGQVRRVKGFHSCCYDCVDCEAGSYRQNPDDIACTFCGQDEWSPERSTRCFRRRSRFLAWGEPAVL |
| 预测分子量 | 68.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TAS1R3重组蛋白的3篇示例参考文献(文献名称与内容为虚构示例，仅作参考模板)：
1. **文献名称**：*Structural insights into the human sweet receptor TAS1R3 through recombinant expression and crystallography*
**作者**：Smith A, et al.
**摘要**：该研究通过重组表达技术在大肠杆菌中成功获得人源TAS1R3蛋白的胞外结构域，并利用X射线晶体学解析其三维结构，揭示了甜味分子(如蔗糖)结合的关键氨基酸位点。
2. **文献名称**：*Functional characterization of TAS1R3/TAS1R2 heterodimer in umami taste perception using a recombinant cell system*
**作者**：Chen L, et al.
**摘要**：作者在HEK293细胞中重组表达了TAS1R3与TAS1R2的异源二聚体，通过钙离子信号检测证实其在鲜味物质(如谷氨酸钠)识别中的核心作用，并发现特定pH值可增强受体活性。
3. **文献名称**：*Development of a high-throughput screening assay for TAS1R3 ligands based on recombinant protein fluorescence labeling*
**作者**：Wang Y, et al.
**摘要**：研究构建了荧光标记的TAS1R3重组蛋白，开发了一种新型配体筛选平台，成功鉴定出多种新型甜味增强剂，为糖尿病友好型甜味剂的设计提供理论基础。
4. **文献名称**：*Role of TAS1R3 in metabolic regulation: Insights from recombinant knockout mouse models*
**作者**：Kim H, et al.
**摘要**：通过基因重组技术构建TAS1R3敲除小鼠模型，发现该受体缺失会导致甜味感知丧失，并进一步影响胰岛素分泌和能量代谢，提示其在代谢疾病中的潜在调控作用。
(注：以上文献为模拟内容，实际研究中请参考真实发表的学术论文。)
The TAS1R3 protein is a critical component of the human taste receptor system, belonging to the class C G protein-coupled receptor (GPCR) family. It functions as a heterodimer, pairing with either TAS1R1 or TAS1R2 to form distinct sweet and umami taste receptors. TAS1R3-TAS1R1 detects L-amino acids (umami taste), while TAS1R3-TAS1R2 recognizes sweet-tasting molecules, including sugars and artificial sweeteners. This receptor’s ability to interact with diverse ligands makes it central to understanding taste perception and metabolic regulation.
Recombinant TAS1R3 proteins are engineered through heterologous expression systems, such as mammalian (HEK293), insect (Sf9), or yeast cells, to study its structure-function relationships. Producing functional TAS1R3 poses challenges due to its complex transmembrane topology and requirement for proper post-translational modifications (e.g., glycosylation) and dimerization. To address this, researchers often co-express TAS1R3 with its partner subunits and G-proteins, supplemented with synthetic liposomes or nanodiscs to stabilize the receptor in vitro.
Studies using recombinant TAS1R3 have revealed ligand-binding domains, activation mechanisms, and species-specific taste preferences. For example, human TAS1R3 exhibits broader sweetener recognition compared to rodents. These insights drive applications in food science (designing healthier sweeteners) and pharmacology (targeting metabolic disorders linked to taste pathways). Additionally, TAS1R3 is implicated in extraoral roles, including gut nutrient sensing and glucose homeostasis, expanding its biomedical relevance.
Despite progress, structural resolution remains limited due to conformational flexibility. Recent cryo-EM advances and mutagenesis studies are overcoming these barriers, enabling drug discovery for obesity and diabetes. Recombinant TAS1R3 thus serves as a vital tool for decoding taste biology and developing therapeutic interventions.
×
