| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF831 |
| Uniprot No | Q5JPB2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1677aa |
| 氨基酸序列 | MEVPEPTCPAPPARDQPAPTPGPPGAPGGQASPHLTLGPVLLPPEQGLAPPTVFLKALPIPLYHTVPPGGLQPRAPLVTGSLDGGNVPFILSPVLQPEGPGPTQVGKPAAPTLTVNIVGTLPVLSPGLGPTLGSPGKVRNAGKYLCPHCGRDCLKPSVLEKHIRSHTGERPFPCATCGIAFKTQSNLYKHRRTQTHLNNSRLSSESEGAGGGLLEEGDKAGEPPRPEGRGESRCQGMHEGASERPLSPGAHVPLLAKNLDVRTEAAPCPGSAFADREAPWDSAPMASPGLPAASTQPWRKLPEQKSPTAGKPCALQRQQATAAEKPWDAKAPEGRLRKCESTDSGYLSRSDSAEQPHAPCSPLHSLSEHSAESEGEGGPGPGPGVAGAEPGAREAGLELEKKRLEERIAQLISHNQAVVDDAQLDNVRPRKTGLSKQGSIDLPTPYTYKDSFHFDIRALEPGRRRAPGPVRSTWTPPDKSRPLFFHSVPTQLSTTVECVPVTRSNSLPFVEGSRTWLEPREPRDPWSRTQKPLSPRPGPARLGCRSGLSSTDVPSGHPRALVRQAAVEDLPGTPIGDALVPAEDTDAKRTAAREAMAGKGRAGGRKCGQRRLKMFSQEKWQVYGDETFKRIYQKMKASPHGGKKAREVGMGSGAELGFPLQKEAAGSSGTVPTQDRRTPVHEDISAGATPEPWGNPPALEASLVTEPTKHGETVARRGDSDRPRVEEAVSSPALGGRDSPCSGSRSPLVSPNGRLELGWQMPPAPGPLKGGDVEAPRPVWPDPKLEGGARGVGDVQETCLWAQTVLRWPSRGSGEDKLPSERKKLKVEDLHSWKQPEPVSAETPGGPTQPASLSSQKQDADPGEVPGGSKESARQVGEPLESSGASLAAASVALKRVGPRDKATPLHPAAPAPAEHPSLATPPQAPRVLSALADNAFSPKYLLRLPQAETPLPLPIPWGPRHSQDSLCSSGWPEERASFVGSGLGTPLSPSPASGPSPGEADSILEDPSCSRPQDGRKGAQLGGDKGDRMATSRPAARELPISAPGAPREATSSPPTPTCEAHLVQDMEGDSHRIHRLCMGSTLARARLSGDVLNPWVPNWELGEPPGNAPEDPSSGPLVGPDPCSPLQPGSFLTALTRPQGVPPGWPELALSSHSGTSRSHSTRSPHSTQNPFPSLKAEPRLTWCCLSRSVPLPAEQKAKAASVYLAVHFPGSSLRDEGPNGPPGSNGGWTWTSPGEGGPAQMSKFSYPTVPGVMPQHQVSEPEWKKGLPWRAKMSRGNSKQRKLKINPKRYKGNFLQSCVQLRASRLRTPTWVRRRSRHPPALEGLKPCRTPGQTSSEIAGLNLQEEPSCATSESPPCCGKEEKKEGDCRQTLGTLSLGTSSRIVREMDKRTVKDISPSAGEHGDCTTHSTAATSGLSLQSDTCLAVVNDVPLPPGKGLDLGLLETQLLASQDSVSTDPKPYIFSDAQRPSSFGSKGTFPHHDIATSVAAVCISLPVRTDHIAQEIHSAESRDHSQTAGRTLTSSSPDSKVTEEGRAQTLLPGRPSSGQRISDSVPLESTEKTHLEIPASGPSSASSHHKEGRHKTFFPSRGQYGCGEMTVPCPSLGSDGRKRQVSGLITRKDSVVPSKPEQPIEIPEAPSKSLKKRSLEGMRKQTRVEFSDTSSDDEDRLVIEI |
| 预测分子量 | 177,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于ZNF831相关研究的假设性参考文献示例(仅供参考，实际文献需通过学术数据库查询)：
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1. **文献名称**：*ZNF831 functions as a tumor suppressor in breast cancer by modulating cell proliferation*
**作者**：Smith J, et al.
**摘要**：研究通过重组ZNF831蛋白体外实验，发现其通过抑制细胞周期相关基因的转录，降低乳腺癌细胞增殖能力。
2. **文献名称**：*Structural characterization of ZNF831 recombinant protein and its DNA-binding specificity*
**作者**：Wang L, et al.
**摘要**：利用重组ZNF831蛋白解析其锌指结构域的三维构象，并通过电泳迁移实验(EMSA)证明其特异性结合特定DNA序列。
3. **文献名称**：*ZNF831 interacts with HDAC1 to regulate epigenetic silencing in leukemia*
**作者**：Kim S, et al.
**摘要**：通过重组蛋白共沉淀实验，发现ZNF831与组蛋白去乙酰化酶HDAC1形成复合物，参与白血病细胞中抑癌基因的表观遗传调控。
4. **文献名称**：*High-yield expression and purification of recombinant ZNF831 in E. coli*
**作者**：Chen X, et al.
**摘要**：优化大肠杆菌表达系统，实现ZNF831重组蛋白的高效可溶性表达，并验证其体外活性可用于高通量药物筛选。
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**提示**：建议通过PubMed或Google Scholar搜索关键词“ZNF831 recombinant”或“ZNF831 function”获取真实文献。如需具体文献协助，请提供更多研究背景。
ZNF831 (Zinc Finger Protein 831) is a member of the zinc finger protein family, characterized by conserved C2H2-type zinc finger domains that facilitate DNA or RNA binding. These proteins are often involved in transcriptional regulation, chromatin remodeling, and cellular differentiation. While the specific biological role of ZNF831 remains under investigation, it is hypothesized to function as a transcription factor, modulating gene expression in pathways related to development, cell proliferation, or stress responses. Genomic studies have linked ZNF831 to diseases such as cancer, with altered expression observed in breast cancer, colorectal cancer, and gliomas, suggesting potential roles in tumorigenesis or metastasis.
Recombinant ZNF831 protein is engineered in vitro using expression systems like *E. coli* or mammalian cells, enabling researchers to study its structure, interactions, and functional mechanisms. The purified protein typically retains DNA-binding activity, making it valuable for electrophoretic mobility shift assays (EMSAs), protein-protein interaction studies, or screening for regulatory targets via chromatin immunoprecipitation (ChIP). Its production often includes affinity tags (e.g., GST, His-tag) for simplified purification and detection.
Current research focuses on elucidating ZNF831’s role in cellular processes and disease contexts. For example, studies explore its interplay with signaling pathways like Wnt/β-catenin or TP53. as well as its potential as a biomarker or therapeutic target. However, functional redundancy with other zinc finger proteins and incomplete pathway mapping remain challenges. Further work is needed to define its precise molecular networks and clinical relevance, underscoring the importance of recombinant ZNF831 as a tool for mechanistic and translational studies.
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