| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HIGD1A |
| Uniprot No | Q9Y241 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-93aa |
| 氨基酸序列 | MSTDTGVSLPSYEEDQGSKLIRKAKEAPFVPVGIAGFAAIVAYGLYKLKS RGNTKMSIHLIHMRVAAQGFVVGAMTVGMGYSMYREFWAKPKP |
| 预测分子量 | 10,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HIGD1A重组蛋白的3篇示例文献(注：以下为虚构示例，实际文献需通过数据库检索)：
1. **文献名称**: "HIGD1A regulates mitochondrial dynamics and apoptosis under hypoxic conditions"
**作者**: Kwon, Y. et al.
**摘要**: 研究揭示HIGD1A重组蛋白通过调控线粒体膜完整性，在缺氧条件下抑制细胞凋亡并维持线粒体功能，可能成为缺血性疾病治疗的靶点。
2. **文献名称**: "Structural and functional characterization of recombinant HIGD1A in cytochrome c oxidase assembly"
**作者**: Lee, S. et al.
**摘要**: 通过体外表达HIGD1A重组蛋白，证实其与细胞色素c氧化酶亚基的互作，阐明其在电子传递链复合体组装中的关键作用。
3. **文献名称**: "Hypoxia-inducible HIGD1A promotes tumor cell survival via ROS scavenging"
**作者**: Smith, J. et al.
**摘要**: 研究发现重组HIGD1A在低氧肿瘤微环境中通过清除活性氧(ROS)抑制氧化应激，增强癌细胞存活能力，提示其促癌机制。
4. **文献名称**: "HIGD1A recombinant protein protects against myocardial ischemia-reperfusion injury"
**作者**: Chen, L. et al.
**摘要**: 动物实验表明，外源性HIGD1A重组蛋白可通过抑制线粒体通透性转换孔(mPTP)开放，减少心肌细胞凋亡，改善缺血再灌注损伤。
建议通过PubMed或Google Scholar以关键词“HIGD1A recombinant protein”检索真实文献。
**Background of HIGD1A Recombinant Protein**
HIGD1A (HIG1 hypoxia inducible domain family member 1A) is a mitochondrial protein encoded by the *HIGD1A* gene, belonging to the HIG1 protein family. It is evolutionarily conserved and primarily localized to the inner mitochondrial membrane, where it plays a critical role in maintaining mitochondrial homeostasis and modulating cellular responses to stress. HIGD1A is implicated in regulating mitochondrial structure, energy metabolism, and apoptosis, particularly under hypoxic conditions. Studies suggest its involvement in hypoxia adaptation by stabilizing respiratory chain complexes and optimizing electron transport efficiency, thereby supporting cell survival during oxygen deprivation.
Recombinant HIGD1A protein is engineered using heterologous expression systems, such as *E. coli* or mammalian cell lines, to produce a purified, functional form of the protein for research. This recombinant tool enables detailed investigation of HIGD1A’s molecular interactions, including its binding partners (e.g., respiratory complex subunits) and its role in stress signaling pathways like the HIF-1α (hypoxia-inducible factor 1-alpha) cascade. Its applications span cancer biology, neurodegenerative diseases, and ischemia-reperfusion injury models, where mitochondrial dysfunction is a key pathological feature.
Recent research highlights HIGD1A’s dual role in apoptosis—promoting survival under mild stress while facilitating cell death under severe conditions—making it a potential therapeutic target. Its recombinant form is also utilized in drug discovery to screen for compounds modulating mitochondrial resilience. Despite progress, mechanisms underlying HIGD1A’s context-dependent functions remain under exploration, underscoring the importance of recombinant protein-based studies to unravel its physiological and pathological significance.
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