| 纯度 | >90%SDS-PAGE. |
| 种属 | mouse |
| 靶点 | cyp51 |
| Uniprot No | Q8K0C4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-503aa |
| 氨基酸序列 | MVLLGLLQSGGWVLGQAMEQVTGGNLLSTLLIACAFTLSLVYLFRLAVGHMVQLPAGAKSPPHIYSPIPFLGHAIAFGKSPIEFLENAYEKYGPVFSFTMVGKTFTYLLGSDAAALLFNSKNEDLNAEEVYGRLTTPVFGKGVAYDVPNAIFLEQKKIIKSGLNIAHFKQYVPIIEKEAKEYFQSWGESGERNVFEALSELIILTASHCLHGKEIRSQLNEKVAQLYADLDGGFTHAAWLLPAWLPLPSFRRRDRAHREIKNIFYKAIQKRRLSKEPAEDILQTLLDSTYKDGRPLTDEEISGMLIGLLLAGQHTSSTTSAWMGFFLAKDKPLQEKCYLEQKAVCGEDLPPLTYDQLKDLNLLDRCIKETLRLRPPIMTMMRMAKTPQTVAGYTIPPGHQVCVSPTVNQRLKDSWAERLDFNPDRYLQDNPASGEKFAYVPFGAGRHRCVGENFAYVQIKTIWSTMLRLYEFDLINGYFPTVNYTTMIHTPENPVIRYKRRSK |
| 预测分子量 | 56,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于CYP51重组蛋白的典型研究文献摘要(基于公开知识库信息，非实时检索结果)：
1. **文献名称**：*"Crystal structure of CYP51 from Mycobacterium tuberculosis in complex with azole inhibitors"*
**作者**：Podust LM 等人
**摘要**：解析结核分枝杆菌CYP51蛋白与多种唑类抑制剂的复合物晶体结构，揭示其底物结合通道特征及抗真菌药物靶向机制，为抗结核药物设计提供结构基础。
2. **文献名称**：*"Functional characterization of recombinant CYP51 from Candida albicans"*
**作者**：Lepesheva GI 等人
**摘要**：通过昆虫细胞表达系统成功重组表达白色念珠菌CYP51蛋白，测定其甾醇14α-去甲基化酶活性，并验证唑类药物对其活性的抑制作用，建立体外酶活检测模型。
3. **文献名称**：*"Optimization of heterologous CYP51A1 expression in E. coli for mechanistic studies"*
**作者**：Fischer RT 等人
**摘要**：优化大肠杆菌表达系统实现人类CYP51A1高效可溶性表达，结合光谱学方法分析酶与甾醇底物的相互作用，阐明催化反应中的电子传递路径。
注：实际文献需通过PubMed/Web of Science等数据库检索确认准确性。建议补充检索关键词："CYP51 recombinant protein expression/structure/function"。
Cyp51. also known as sterol 14α-demethylase, is a key enzyme in the cytochrome P450 superfamily, playing a central role in sterol biosynthesis across eukaryotes. It catalyzes the removal of the 14α-methyl group from lanosterol or eburicol in fungi (yielding ergosterol) or from lanosterol in animals (yielding cholesterol), a critical step in membrane integrity and cellular function. Due to its essential role in fungal ergosterol synthesis, Cyp51 is a primary target for azole-class antifungal drugs, which inhibit enzyme activity by binding to its heme-coordinated active site.
Recombinant Cyp51 proteins are engineered through heterologous expression systems (e.g., *E. coli*, yeast, or insect cells) to study enzyme structure, function, and drug interactions. These systems enable large-scale production of purified protein for biochemical assays, structural analysis (via X-ray crystallography or cryo-EM), and high-throughput drug screening. Fungal Cyp51 variants associated with azole resistance—often due to mutations or overexpression—are particularly studied to decipher resistance mechanisms and design next-generation inhibitors.
In human health, human Cyp51 mutations are linked to developmental disorders, highlighting its physiological importance. Recombinant forms also aid in exploring host-pathogen interactions and species-specific differences in sterol pathways. Beyond biomedicine, plant Cyp51 homologs are investigated for their roles in phytoalexin biosynthesis and stress responses. Overall, recombinant Cyp51 serves as a vital tool for antifungal development, mechanistic enzymology, and understanding sterol-related diseases.
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