Recombinant Human DDX3X protein

DDX3X is a member of the DEAD-box RNA helicase family, characterized by its conserved Asp-Glu-Ala-Asp (DEAD) motif. Encoded by the DDX3X gene located on the X chromosome, this protein plays critical roles in RNA metabolism, including splicing, transport, translation, and ribosome assembly. It unwinds RNA secondary structures in an ATP-dependent manner, facilitating interactions between RNA and proteins or other nucleic acids. DDX3X is ubiquitously expressed and implicated in diverse cellular processes, such as cell cycle regulation, stress response, and innate immunity. Dysregulation of DDX3X has been linked to cancers (e.g., medulloblastoma, leukemia), neurodevelopmental disorders, and viral infections, underscoring its biological and clinical significance.

Recombinant DDX3X protein is engineered for research and therapeutic applications. Produced via heterologous expression systems (e.g., E. coli, mammalian cells), it retains enzymatic activity and structural integrity, enabling in vitro studies of its molecular mechanisms. Purification typically involves affinity tags (e.g., His-tag) followed by chromatography. Researchers use recombinant DDX3X to investigate its interactions with RNA, viral proteins (e.g., HIV-1. hepatitis C), or host signaling pathways (e.g., Wnt/β-catenin, NF-κB). It also serves as a tool for screening antiviral or anticancer compounds targeting its helicase activity. Recent studies highlight its potential as a biomarker or therapeutic target, particularly in cancers with DDX3X mutations or viral replication pathways.