| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GJA1 |
| Uniprot No | P17302 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 233-382aa |
| 氨基酸序列 | FKGVKDRVKGKSDPYHATSGALSPAKDCGSQKYAYFNGCSSPTAPLSPMSPPGYKLVTGDRNNSSCRNYNKQASEQNWANYSAEQNRMGQAGSTISNSHAQPFDFPDDNQNSKKLAAGHELQPLAIVDQRPSSRASSRASSRPRPDDLEI |
| 预测分子量 | 19.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GJA1(Connexin 43)重组蛋白研究的模拟参考文献示例(非真实文献，仅供格式参考)：
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1. **标题**：Expression and purification of recombinant human GJA1 in Escherichia coli for structural studies
**作者**：Zhang et al.
**摘要**：研究报道了通过大肠杆菌表达系统成功表达并纯化重组GJA1蛋白，优化了可溶性表达条件，并通过尺寸排阻色谱和圆二色光谱验证了蛋白的正确折叠，为后续结构解析奠定基础。
2. **标题**：Functional characterization of GJA1 hemichannels in a mammalian cell line model
**作者**：Smith et al.
**摘要**：利用哺乳动物细胞系(HEK293)重组表达GJA1蛋白，通过膜片钳技术分析其半通道活性，发现特定突变(如R76H)显著影响通道通透性，提示其在遗传性疾病中的潜在机制。
3. **标题**：Cryo-EM structure of the human Cx43 gap junction channel in lipid nanodiscs
**作者**：Johnson et al.
**摘要**：首次通过冷冻电镜解析了重组GJA1蛋白在脂质纳米盘中形成的完整间隙连接通道结构，揭示了其跨膜区及胞内调控域的构象变化，为靶向药物设计提供结构基础。
4. **标题**：Recombinant GJA1 improves cardiac repair in a myocardial infarction model
**作者**：Wang et al.
**摘要**：在小鼠心肌梗死模型中，注射重组GJA1蛋白可增强心肌细胞间通讯，减少纤维化并促进功能恢复，表明其潜在治疗价值。
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实际文献需通过PubMed、Web of Science或Google Scholar等平台检索关键词(如“recombinant GJA1 protein”或“Connexin 43 expression”)获取。
GJA1. also known as connexin 43 (Cx43), is a member of the connexin family of transmembrane proteins that form gap junctions—specialized intercellular channels facilitating direct communication between adjacent cells. These channels enable the exchange of ions, metabolites, and signaling molecules, playing critical roles in maintaining tissue homeostasis, coordinating cellular activities, and regulating developmental processes. GJA1 is widely expressed in various tissues, including the heart, skin, nervous system, and bone, and its dysfunction is linked to cardiovascular anomalies, skin disorders, hearing loss, and cancer.
Recombinant GJA1 protein is engineered through molecular cloning techniques, typically expressed in prokaryotic (e.g., *E. coli*) or eukaryotic systems (e.g., mammalian cells) to produce functional or truncated forms for research and therapeutic applications. The protein’s structure includes four transmembrane domains, two extracellular loops, and intracellular N- and C-termini. Post-translational modifications, such as phosphorylation, regulate its channel gating, trafficking, and degradation.
Recombinant GJA1 is pivotal in studying gap junction biology, disease mechanisms, and drug discovery. It aids in elucidating how mutations (e.g., oculodentodigital dysplasia-linked variants) disrupt intercellular communication. Additionally, it serves as a tool to explore therapeutic strategies, such as enhancing wound healing, mitigating cardiac arrhythmias, or targeting cancer cell proliferation. Innovations in protein engineering and delivery systems continue to expand its potential in regenerative medicine and precision therapies.
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