| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RECQL |
| Uniprot No | P46063 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-649aa |
| 氨基酸序列 | MASVSALTEELDSITSELHAVEIQIQELTERQQELIQKKKVLTKKIKQCLEDSDAGASNEYDSSPAAWNKEDFPWSGKVKDILQNVFKLEKFRPLQLETINVTMAGKEVFLVMPTGGGKSLCYQLPALCSDGFTLVICPLISLMEDQLMVLKQLGISATMLNASSSKEHVKWVHAEMVNKNSELKLIYVTPEKIAKSKMFMSRLEKAYEARRFTRIAVDEVHCCSQWGHDFRPDYKALGILKRQFPNASLIGLTATATNHVLTDAQKILCIEKCFTFTASFNRPNLYYEVRQKPSNTEDFIEDIVKLINGRYKGQSGIIYCFSQKDSEQVTVSLQNLGIHAGAYHANLEPEDKTTVHRKWSANEIQVVVATVAFGMGIDKPDVRFVIHHSMSKSMENYYQESGRAGRDDMKADCILYYGFGDIFRISSMVVMENVGQQKLYEMVSYCQNISKCRRVLMAQHFDEVWNSEACNKMCDNCCKDSAFERKNITEYCRDLIKILKQAEELNEKLTPLKLIDSWMGKGAAKLRVAGVVAPTLPREDLEKIIAHFLIQQYLKEDYSFTAYATISYLKIGPKANLLNNEAHAITMQVTKSTQNSFRAESSQTCHSEQGDKKMEEKNSGNFQKKAANMLQQSGSKNTGAKKRKIDDA |
| 预测分子量 | 73.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RECQL重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*Human RECQL1: Cloning, Expression, and Functional Studies*
**作者**：Mendoza-Maldonado R. et al.
**摘要**：该研究成功克隆并重组表达了人源RECQL1蛋白，验证其ATP依赖的DNA解旋酶活性，发现其能特异性解开DNA双链结构，并在体外修复实验中促进同源重组修复。
2. **文献名称**：*RECQL5 Helicase: Roles in Genomic Stability and Tumor Suppression*
**作者**：Hu Y. et al.
**摘要**：研究揭示了RECQL5通过抑制非编码RNA-DNA杂交结构(R-loop)的形成维持基因组稳定性，重组RECQL5蛋白的体外实验表明其与RNA聚合酶结合，减少DNA损伤和癌症风险。
3. **文献名称**：*Functional Interaction Between RECQL and PARP1 in DNA Repair*
**作者**：Li M. et al.
**摘要**：本文证明重组RECQL蛋白与PARP1协同参与DNA单链断裂修复，RECQL通过解旋酶活性辅助PARP1激活下游修复通路，缺失RECQL导致修复效率显著降低。
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以上文献聚焦RECQL家族蛋白的重组表达、酶学功能及其在DNA修复中的机制，涵盖肿瘤抑制与基因组稳定性研究。
RECQL, a member of the RecQ helicase family, is a conserved DNA helicase critical for maintaining genomic stability. First identified in the mid-1990s, RECQL (also called RECQL1 or RecQ protein-like 1) is one of five human RecQ homologs, alongside BLM, WRN, RECQL4. and RECQL5. These helicases are renowned for their roles in DNA repair, replication, recombination, and telomere maintenance. RECQL specifically unwinds duplex DNA and RNA-DNA hybrids in an ATP-dependent manner, resolving secondary structures that impede replication or repair processes.
Structurally, RECQL contains a conserved helicase domain with ATP-binding and catalytic motifs, flanked by N- and C-terminal regions involved in protein interactions. Unlike BLM or WRN, which are linked to specific genetic disorders (Bloom and Werner syndromes), RECQL deficiencies are not directly tied to monogenic diseases. However, studies associate RECQL dysregulation with cancer susceptibility. For example, RECQL overexpression is observed in colorectal and breast cancers, while its downregulation correlates with increased replication stress and genomic instability.
Recombinant RECQL proteins, produced via bacterial or eukaryotic expression systems, are widely used to study helicase mechanisms. Purified RECQL exhibits ATPase activity and unwinding proficiency on substrates like forked DNA, Holliday junctions, and G-quadruplexes. Its interaction with key partners (e.g., PARP1. PCNA, and RPA) highlights its role in homologous recombination repair and replication fork restart.
Research on recombinant RECQL also explores therapeutic potential. Small-molecule inhibitors targeting RECQL’s ATPase activity are under investigation for cancer treatment, while RECQL supplementation is proposed to mitigate replication defects in aging or chemotherapy-exposed cells. Overall, RECQL remains a pivotal focus in understanding DNA repair pathways and developing strategies against genome instability-related diseases.
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