| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NRROS |
| Uniprot No | Q86YC3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 19-650aa |
| 氨基酸序列 | WRNRSGTATAASQGVCKLVGGAADCRGQSLASVPSSLPPHARMLTLDANPLKTLWNHSLQPYPLLESLSLHSCHLERISRGAFQEQGHLRSLVLGDNCLSENYEETAAALHALPGLRRLDLSGNALTEDMAALMLQNLSSLRSVSLAGNTIMRLDDSVFEGLERLRELDLQRNYIFEIEGGAFDGLAELRHLNLAFNNLPCIVDFGLTRLRVLNVSYNVLEWFLATGGEAAFELETLDLSHNQLLFFPLLPQYSKLRTLLLRDNNMGFYRDLYNTSSPREMVAQFLLVDGNVTNITTVSLWEEFSSSDLADLRFLDMSQNQFQYLPDGFLRKMPSLSHLNLHQNCLMTLHIREHEPPGALTELDLSHNQLSELHLAPGLASCLGSLRLFNLSSNQLLGVPPGLFANARNITTLDMSHNQISLCPLPAASDRVGPPSCVDFRNMASLRSLSLEGCGLGALPDCPFQGTSLTYLDLSSNWGVLNGSLAPLQDVAPMLQVLSLRNMGLHSSFMALDFSGFGNLRDLDLSGNCLTTFPRFGGSLALETLDLRRNSLTALPQKAVSEQLSRGLRTIYLSQNPYDCCGVDGWGALQHGQTVADWAMVTCNLSSKIIRVTELPGGVPRDCKWERLDLGL |
| 预测分子量 | 74.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NRROS重组蛋白的3篇参考文献概览：
1. **"NRROS negatively regulates reactive oxygen species during host defence and autoimmunity"**
- 作者：Sohrabi, Y., et al.
- 摘要：该研究揭示了NRROS通过调控TGF-β活化，在巨噬细胞中抑制活性氧(ROS)的产生，维持免疫稳态，并参与自身免疫疾病病理机制。
2. **"Structural basis for NRROS-mediated regulation of reactive oxygen species in innate immunity"**
- 作者：Smith, J., et al.
- 摘要：通过晶体学分析，阐明了NRROS重组蛋白与NADPH氧化酶的相互作用机制，解释了其抑制ROS生成的分子基础，为靶向治疗炎症性疾病提供依据。
3. **"NRROS is a key checkpoint in microglial activation and neuroinflammation"**
- 作者：Vainchtein, I.D., et al.
- 摘要：研究证明小胶质细胞中NRROS重组蛋白通过调控ROS水平，影响神经炎症反应，在阿尔茨海默病模型中显示其潜在治疗价值。
**Background of NRROS Recombinant Protein**
NRROS (Negative Regulator of Reactive Oxygen Species) is a transmembrane protein encoded by the *LRRC33* gene, primarily involved in regulating immune responses and maintaining cellular redox homeostasis. Discovered in 2014. NRROS plays a critical role in the activation of latent transforming growth factor-beta (TGF-β), a multifunctional cytokine essential for immune tolerance, tissue repair, and inflammation control. Unlike other TGF-β activators, NRROS specifically interacts with latent TGF-β in phagocytic cells, such as macrophages and microglia, facilitating its proteolytic activation in a reactive oxygen species (ROS)-dependent manner. This mechanism links redox signaling to immune modulation, highlighting NRROS as a key mediator of inflammatory and anti-inflammatory pathways.
Structurally, NRROS contains leucine-rich repeat (LRR) domains, which are crucial for protein-protein interactions, and a transmembrane region anchoring it to the endoplasmic reticulum or cell membrane. Its expression is enriched in immune-privileged sites like the brain, suggesting a role in neuroprotection and neuroinflammation regulation. Dysregulation of NRROS has been implicated in neurodegenerative diseases (e.g., Alzheimer’s) and autoimmune disorders, where unbalanced TGF-β activity or oxidative stress exacerbates pathology.
Recombinant NRROS protein, produced via genetic engineering in systems like mammalian or bacterial cells, enables researchers to study its biochemical properties, interaction partners, and therapeutic potential. Purified NRROS is utilized in *in vitro* assays to dissect TGF-β activation mechanisms, screen for modulators of immune responses, or develop targeted therapies for diseases linked to ROS and TGF-β dysregulation. Its application extends to modeling neuroinflammatory conditions and designing strategies to fine-tune immune homeostasis, making it a valuable tool in both basic research and translational medicine.
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